This document provides the procedure for performing ABO blood grouping at Chitungwiza Central Hospital laboratory in Zimbabwe. It describes the purpose, responsibilities, equipment, principle, and method for determining a patient's blood group. The method involves testing red blood cells or serum against anti-A, anti-B, and anti-AB reagents to observe agglutination, which will identify if the blood is type A, B, AB, or O based on a provided interpretation chart.
This document provides the procedure for performing ABO blood grouping at Chitungwiza Central Hospital laboratory in Zimbabwe. It describes the purpose, responsibilities, equipment, principle, and method for determining a patient's blood group. The method involves testing red blood cells or serum against anti-A, anti-B, and anti-AB reagents to observe agglutination, which will identify if the blood is type A, B, AB, or O based on a provided interpretation chart.
This document provides the procedure for performing ABO blood grouping at Chitungwiza Central Hospital laboratory in Zimbabwe. It describes the purpose, responsibilities, equipment, principle, and method for determining a patient's blood group. The method involves testing red blood cells or serum against anti-A, anti-B, and anti-AB reagents to observe agglutination, which will identify if the blood is type A, B, AB, or O based on a provided interpretation chart.
This document provides the procedure for performing ABO blood grouping at Chitungwiza Central Hospital laboratory in Zimbabwe. It describes the purpose, responsibilities, equipment, principle, and method for determining a patient's blood group. The method involves testing red blood cells or serum against anti-A, anti-B, and anti-AB reagents to observe agglutination, which will identify if the blood is type A, B, AB, or O based on a provided interpretation chart.
Name of Laboratory : Chitungwiza Central Hospital Revision Number : 0
Department : Quality Effective Date : 21/09/2011
Document Number : BB1 Page number : 1 of 2 Title : ABO Grouping Procedure Authorized By :
1. PURPOSE: To find patients blood group.
2. RESPONSIBILITY : Medical Laboratory Scientist/Medical Laboratory Technician.
SPECIMEN : EDTA or clotted blood sample can be used.
3. EQUIPMENT : 75mm glass or disposable test tubes. Pasteur pipettes Blood grouping racks Centrifuge Saline wash bottles
4. PRINCIPLE Based on the principle of agglutination. Red cells with the antigen will agglutinate when tested against the corresponding antibody.
5. METHOD
Cell Grouping 1. Label tubes with laboratory number and test identification (anti A, B and AB). 2. Prepare cell suspension for cells being tested. 3. Place two drops of anti A, anti B, and anti AB reagent in the appropriately labeled tubes. 4. Add to each tube one drop of 2-5% cell suspension (in normal saline, serum or plasma of the red cells to be tested). 5. Centrifuge at 1500rpm for 30seconds and observed for agglutination.
Serum Grouping 1. Label the tubes with test identification (A, B and O). 2. Add two drops of test serum in all tubes in the corresponding column. 3. Add one drop of 2-5% group A cell suspension in tube labeled A. 4. Add one drop of 2-5% group B cell suspension in tube labeled B. 5. Add one drop of 2-5% group O cell suspension in tube labeled O. 6. Centrifuge at 1500rpm for 30seconds and observe for agglutination.
6. RESULTS:
Interpretation
Anti A Anti B Anti AB A B O Group + - + - + - A - + + + - - B - - - + + - O + + + - - - AB