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3300 Chem. Analyzer Service Manual
3300 Chem. Analyzer Service Manual
3300 Chem. Analyzer Service Manual
3300
Service Manual
Copyright 2004 Awareness Technology Inc. 01/2008 Doc. 3300 Rev. G
TABLE OF CONTENTS
1. INTRODUCTION................................................................................................................................... 1
1.1 APPLICATIONS...................................................................................................................................... 1
1.1.1 Intended Use ................................................................................................................................ 1
1.1.2 Summary of the Instrument .......................................................................................................... 1
1.2 SPECIFICATIONS.................................................................................................................................... 2
1.3 WARNING MARKINGS........................................................................................................................... 5
1.3.1 Safety Symbols ............................................................................................................................. 5
1.3.2 Safety Terms................................................................................................................................. 5
1.4 SAFETY PRECAUTIONS.......................................................................................................................... 5
2. PRINCIPLES OF OPERATION........................................................................................................... 8
2.1 POWER REQUIREMENTS........................................................................................................................ 8
2.2 PARTS AND CONTROLS......................................................................................................................... 9
2.3 GENERAL LAYOUT.............................................................................................................................. 11
2.3.1 The Chassis ................................................................................................................................ 11
2.3.2 Front Panel Electronics ............................................................................................................. 11
2.3.3 Main PCB .................................................................................................................................. 12
2.3.4 Photometer Mechanism.............................................................................................................. 14
2.3.5 Flowcell ..................................................................................................................................... 17
2.3.6 Valve .......................................................................................................................................... 17
2.3.7 Vacuum Pump and Pump Control Circuit ................................................................................. 17
2.3.8 Temperature Control.................................................................................................................. 18
2.4 PARALLEL INTERFACE........................................................................................................................ 18
2.5 AUXILIARY AND OPTIONAL EQUIPMENT............................................................................................. 18
2.5.1 Auxiliary Equipment Interface ................................................................................................... 18
2.5.2 Optional Equipment ................................................................................................................... 18
3. SERVICE PROCEDURES................................................................................................................... 19
3.1 OPENING THE INSTRUMENT ................................................................................................................ 19
3.2 CLEANING THE FLOWCELL ................................................................................................................. 21
3.3 FLOWCELL CONFIGURATION .............................................................................................................. 22
3.4 FLOWCELL ALIGNMENT...................................................................................................................... 24
3.5 FLOWCELL TUBING REPLACEMENT .................................................................................................... 25
3.6 TESTING FOR LEAKS IN THE FLOWCELL ASSEMBLY............................................................................ 32
3.7 FLOWCELL CELL INSERT REPLACEMENT............................................................................................ 34
3.8 MAIN PCB AND/OR PHOTOMETER REPLACEMENT.............................................................................. 35
3.8.1 Photometer Replacement ........................................................................................................... 35
3.8.2 Main PCB Replacement ............................................................................................................. 37
3.9 VALVE TUBING REPLACEMENT .......................................................................................................... 38
3.10 EPROM REPLACEMENT ................................................................................................................... 39
3.11 FILTER CHANGE PROCEDURE ........................................................................................................... 41
3.12 LAMP REPLACEMENT........................................................................................................................ 44
3.13 VACUUM ADJ USTMENT .................................................................................................................... 46
3.14 EXHAUST FILTER REPLACEMENT...................................................................................................... 47
4. TROUBLESHOOTING........................................................................................................................ 48
4.1 KEYPAD PROBLEMS............................................................................................................................ 48
4.1.1 No Keys Function....................................................................................................................... 48
4.1.2 A Row or Column of the Keypad Does Not Function................................................................. 48
4.1.3 The Keyboard and Display Do Not Function............................................................................. 48
4.1.4 Keypad Key Stuck ...................................................................................................................... 48
4.1.5 Password Protection Override................................................................................................... 48
4.2 PHOTOMETER PROBLEMS.................................................................................................................... 49
4.2.1 Lamp Low/Filter Low Message is Displayed............................................................................. 49
4.2.2 Filter Wheel Does Not Rotate .................................................................................................... 49
4.2.3 Filter Wheel Runs at an Uncontrolled Rate............................................................................... 49
4.2.4 Incorrect Control Readings ....................................................................................................... 50
4.2.5 Poor Linearity............................................................................................................................ 50
4.2.6 Erratic Readings ........................................................................................................................ 50
4.2.7 Lamp failure............................................................................................................................... 50
4.2.8 No sampling ............................................................................................................................... 50
5. CALIBRATION ..................................................................................................................................... 51
5.1 CALIBRATION AND ABSORBANCE LINEARITY..................................................................................... 52
5.2 RESTORE CALIBRATION DATA............................................................................................................ 52
5.3 TEMPERATURE CALIBRATION............................................................................................................. 53
5.4 ABSORBANCE CALIBRATION............................................................................................................... 54
5.5 RESTORE FILTER LABELS ................................................................................................................... 55
6. CONTACT INFORMATION ............................................................................................................... 56
APPENDIX 1 SCHEMATICS................................................................................................................ 57
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Spectrophotometer Type ........................... Filter photometer
Optical Configuration ................................. Single beam with continuously rotating
filter wheel
Monochromatic or bichromatic reading
8 filter positions
Usable Spectral Range.............................. 330 to 770 nm
System Procedures ................................... Open and by stored menu
Calculating Modes .................................... Absorbance
Single Standard
Differential samples
Factor Mode
Differential samples
Multi Standard Mode (up to 7
standards)
Multi Standard % Abs (up to 7
standards)
Kinetic Mode (consecutively, or
simultaneously (Batch))
By Factor or by Standard
Fixed Time Kinetic
By Factor or by Standard
Index Mode
Channels ................................................... 120 Open
Source of Radiation ................................... Tungsten Halogen, 10 Watt, with
automatic lamp saver
Selection of Wavelength............................ By filter
Filter Type.................................................. 4-cavity interference, long-life ion
beam-assisted deposition
Wavelength Accuracy................................ +/- 3 nm
Filter Location............................................ After sample (heat absorbing filter
before sample)
Filter Selection........................................... Automatic by software or via keyboard
Wavelengths.............................................. 340, 405, 505, 545, 580, 630 nm
supplied standard
other/additional filters optional
Half Bandwidth........................................... < 10 nm
1/100 Bandwidth........................................ 14 nm at 340 nm
False Radiant Energy Ratio....................... < 0.001 at 340 and 405 nm
Cuvette ................................................... 1 cm square, 12 cm round, flow through
Supplied Type............................................ Flow-through
Material ...................................................... 316 stainless, borosilicate windows
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Figure 2.2-2 Rear view
A
B
D
C
E
I
J
H
F
G
K
L
M
N
O
W
Figure 2.2-1 Overall view
10 Stat Fax
3300 uses a single channel silicon diode detector to read the light
signal from a 6-volt gas-filled lens-end tungsten lamp. The continuously rotating
filter wheel contains four fixed wavelength interference filters, and is located
inside the photometer housing underneath the microwell plate. This arrangement
greatly reduces problems due to ambient light and allows for an open frame
structure of the plate transport system.
The photometer is operated under microprocessor control. The filter wheel is
electronically controlled via a TDA7275A speed control chip to four revolutions
per second. Infrared opto switches provide commutation (filter identification)
using the holes in the edge of the filter wheel. One pair provides a count pulse,
and the other provides a home indication. Figure 2.3.4-1 shows the photometer
mechanism. Figure 2.3.4-2 illustrates the photometer and absorbance
conversion signals (filter count, home, sample and hold, filter peaks and
comparator output waveforms).
The photometer mechanism consists of four elements:
The filter wheel and rotation mechanism: The filter wheel is rotated by
a small DC motor connected to the wheel by a rubber belt. The wheel
speed is electronically controlled to four revolutions per second. As the
wheel turns, the filters mounted in it pass between the light source and
the photo detector, the reading is taken when the filter passes through
the light beam.
Filter wheel position detection system: Optical gates (beam interrupt
switches) are used to control the filter wheel speed and take readings.
Normally the infrared light from the LCD side of the gate is blocked, but
when the small holes in the filter wheel pass by, the light passes through
and the microprocessor receives a signal. There are two sets of holes.
One set consists of several holes, one at each filter. When one of these
holes is detected, the filter is lined up, and a reading is taken. The other
set is only one hole and it is used primarily for synchronizing the filter
count.
Light source: The light source consists of the lamp with its built-in lens,
the diffuser, and the aperture block. The light, originating at the lamp,
passes through the diffuser, then through the sample plate, the aperture
block, and then into the photometer. This area under the spot of light is
known as the read site. The connection to the lamp and filter wheel motor
is the junction PCB with an IDC (insulation displacement connector.)
Optical detection system: This system consists of a photometer PCB
on which is mounted: a light sensitive photodiode and the operation
amplifier. The optical system is connected to the junction PCB using a
14-pin (2 x 7) header cable assembly.
Awareness Technology Inc. Stat Fax
3300:
Pin # Function Direction
1 Strobe To Printer
2 Data0 To Printer
3 Data1 To Printer
4 Data2 To Printer
5 Data3 To Printer
6 Data4 To Printer
7 Data5 To Printer
8 Data6 To Printer
9 Data7 To Printer
11 Busy From Printer
12 Paper Out From Printer
16 Initialize To Printer
19-25 Ground
2.5 Auxiliary and Optional Equipment
2.5.1 Auxiliary Equipment Interface
Parallel Port: The Stat Fax
3300 in
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