Double-Beam Spectrophotometer

Introduction

Principle

What is the Spectrophotometry?

Purpose of the instrument.

Types of Spectrophotometer.
Device Description

Mirror.
Light Source.
Monochromator.
Entrance and exit slit.
Rotating Discs.
Device Description

Sample cell & Reference cell in cuvettes.
Photo Detector (PMT).
Computer.
Optical Wedge.


Applications
Determination of Alanine Aminotransferase
(ALT/GPT) in clinical samples.
Determination of cholesterol in clinical
samples.
Determination of glucose in clinical samples.
Determination of urea in clinical samples.
Determination of phosphate in clinical
samples.
End of the presentation


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