Parrilla is best known for her roles on television.

She was regular cast member on the season 5

of ABC sitcom, Spin City, from 2000 to 2001. She later starred in the short-lived
dramas Boomtown (2002-2003), Windfall (2006), Swingtown (2008) and Miami Medical(2010). She
also played the role of Sarah Gavin on the season four of Fox series 24 in 2005. In 2011, Parrilla
began starring as The Evil Queen/Regina Mills in the ABC fantasy drama series, Once Upon a Time.