1

Grams stain

1.

Grams stain

2.
3.

OF =

Oil immersion Field

(cell wall)

1. Gram positive ()

prosaniline dye Crystal Violet (CV)

Crystal Violet Mordanting agent Gram s Iodine (I)
complex Crystal Violet Iodine

Complex (CVI complex)

(Decolorization) cecolorizer agent Alcohol

Acetone Teichoic acid cross-links
decolorizer pore size

peptidoglycan
CVI complex

2. Gram negative ()

Vibrio sp. Pseudomonas sp.

peptidogly cross-link
pore size
decolorizer agent pore size

CVI complex

Decolorization
counterstain Safranin

4.

4.1 Sputum
4.2 Pus

4.3 CSF
4.4 Body Fluid
4.5 Urine

5.

5.1
5.2

6.

6.1

-
- forcep

6.2
- Gram s stain
Crystal violet
Gram iodine

95 % alcohol (Decolorize)
7.

Safranin O

7.1 smear film

7.2 Fix slide 2

7.3 Crystal violet Smear 1

7.4

7.5 Gram iodine 1

7.6

7.7 95 % alcohol 2
crystal violet
7.8

7.9 Safranin O 30
7.10

7.11

7.12 , ,

FR LAB - 012
8.

8.1
(IQC)

- Gram negative bacilli E. coli smear

slide

- Gram positive cocci S. aureus smear

slide

FR-LAB-029

-
FR-LAB-067

8.2 (EQA)
3
9.

9.1
- Gram positive

- Gram negative

9.2
- Cocci

- Diplococci

- Coccobacilli
- Bacilli

- Bacilli with pleomorphic

- Bacilli with terminal spore

- Bacilli with subterminal spore

- Bacilli with central spore

- Bacilli with subterminal and central spore

- Bacilli with metachromatic granule
- Bacilli with bipolarstaning

9.3

- Bacteria WBC

1+ (rare)

Bacteria

2+ (few)

Bacteria

3+ (Moderate)

Bacteria

4+ (Many)

Bacteria

WBC 0 -1 cells/OF

WBC > 1 - 5 cells/OF

WBC > 5 - 30 cells/OF

WBC >30 cells/OF
10.

HIV, HBsAg

Biosafety Carbinet

11.

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