1

1.

Disc diffusion method

Disc

diffusion method

2.
MHSA
blood

MHA
=

Mueller Hinton agar with 5% sheep

Susceptible

Intermediate

Mueller Hinton agar

Resistant

3.
paper

disc

spread
paper disc paper disc

35 C

(inhibition zone)
Standard
interpretive chart (susceptible, S)

( intermediate, I ) ( resistant , R )

4.
4.1

- Incubator 35 C

- Forcep

4.2

- McFarland standard No. 0.5

- Antimicrobial Agent
5.

- MHA , MHSA

5.1

Sterile Saline McFarland standard No.

0.5

5.2

Sterile swab tube

swab

60 2
plate 3-5

5.3

Forcep 95 % alcohol

forcep

forcep

90
6
inhibition zone
5.4

incubate 35 C 18-24

antimicrobial agent incubate 35 C

24 Vancomycin, Oxacillin
Cefoxitin
5.5

- inhibition zone

2 zone
diameter R (Resistant), I

(Intermediate) S (Susceptible)

zone

( zone

Background)

Trimethoprim/sulfamethoxazole
zone

zone 20%

zone

- Proteus spp. swarming

inhibition zone
swarming

- Beta-hemolytic streptococci

zone zone

5.6

hemolysis

FR-

LAB-012
6.

6.1 (EQA)
6.1.1 3

6.1.2 3
6.2 (IQC) WI-MCB-036

Quality Control Chart :
Antimicrobial susceptible
7.
7.1

(Susceptible, S) inhibition zone

7.2

(Intermediate, I)

inhibition zone

7.3

(Resistant, R) zone

zone

8.

HIV, HBsAg

9.
9.1

-20 C

28 C 1 -6 disk

9.2
9.3

6 plate

inhibition zone

15

1.

. .

, 2544 229-259

2. Clinical and Laboratory Standards Institute. Performance

Standards for Antimicrobial Susceptibility Testing.
: Clinical diagnostic LTD. Part, 2009

3.

DR. M.K.Lalitha. Manual on Antimicrobial

Susceptibility Testing.