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Stain Primary Stain Decolorizer Counterstain Used in class

Gram Stain Crystal violet 95% EtOH Safranin E. coli


Staph. xylosus,
Bacillus
megaterium
Acid Fast Stain Carbol Fuchsin Acid Alcohol Methylene lue Mycobacterium
smegmatis
S!ore Stain Malachite
Green
"a! #ater Safranin Bacillus
megaterium
Ca!sule Stain Crystal violet $%% co!!er
sulfate
$%% co!!er
sulfate
Enterobacter
aerogenes
Media Substrate pH
indicator
Selective or
Differential
Gram
rxn
Other inredients
Mueller&
Hinton
'one 'one 'either ()& 'one
MSA Mannitol *henol red oth ( +,5% 'aCl
lood 'one 'one -ifferential ()& 5% shee!.s blood
MacCon/ey 0actose 'eutral red oth & Crystal violet1 bile
salts
EM 0actose 'one oth & Eosin1 methylene
blue
CC'A 'one 'one oth ( Colistin1 nalidi2ic
acid
Chocolate 'one 'one 'either ()& Coa3ulated blood
Sabouraud.s 'one 'one 'either ()& Acidic !H for fun3i
3ro4th
Snyder 3lucose rom cresol
3reen
()&

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