This experiment uses variegated leaves from Coleus plants that were exposed to light or kept in darkness to test for the presence of starch. The leaves were boiled in water and alcohol to remove pigments, then placed in iodine solution. Iodine turns blue-black in the presence of starch. The light leaf turned entirely blue-black, indicating starch was present everywhere chlorophyll had been. The dark leaf did not change color, showing an absence of starch, as the leaf could not perform photosynthesis without light to produce glucose. This provides indirect evidence that photosynthesis produces starch.
This experiment uses variegated leaves from Coleus plants that were exposed to light or kept in darkness to test for the presence of starch. The leaves were boiled in water and alcohol to remove pigments, then placed in iodine solution. Iodine turns blue-black in the presence of starch. The light leaf turned entirely blue-black, indicating starch was present everywhere chlorophyll had been. The dark leaf did not change color, showing an absence of starch, as the leaf could not perform photosynthesis without light to produce glucose. This provides indirect evidence that photosynthesis produces starch.
This experiment uses variegated leaves from Coleus plants that were exposed to light or kept in darkness to test for the presence of starch. The leaves were boiled in water and alcohol to remove pigments, then placed in iodine solution. Iodine turns blue-black in the presence of starch. The light leaf turned entirely blue-black, indicating starch was present everywhere chlorophyll had been. The dark leaf did not change color, showing an absence of starch, as the leaf could not perform photosynthesis without light to produce glucose. This provides indirect evidence that photosynthesis produces starch.
This experiment uses variegated leaves from Coleus plants that were exposed to light or kept in darkness to test for the presence of starch. The leaves were boiled in water and alcohol to remove pigments, then placed in iodine solution. Iodine turns blue-black in the presence of starch. The light leaf turned entirely blue-black, indicating starch was present everywhere chlorophyll had been. The dark leaf did not change color, showing an absence of starch, as the leaf could not perform photosynthesis without light to produce glucose. This provides indirect evidence that photosynthesis produces starch.
of Photosynthesis 1A PH Group 3 PROCEDURE Obtain two variegated leaves of Coleus (mayana): a light leaf from light-exposed plant and dark leaf from another plant exposed to darkness for 3-4 days. To identify the two, cut off the petiole for the dark leaf. Sketch the variegation pattern of the leaves in column A of question sheet. Then, place both in boiling water for three minutes. Get the leaves and spread them in a Petri dish containing a small amount of water. Sketch any change in the variegation pattern in column B.
Transfer both leaves in boiling 95% alcohol for five minutes. Spread in the Petri dish with water, and sketch any change in column C. Add four drops of iodine solution to the plate, agitate gently, and observe the color reaction. Sketch in column D. RESULTS
Dark brown Dark brown DISCUSSION Anthocyanins are vacuolar pigments soluble in water. They may appear red, blue, or violet. The violet area of the mayana leaf mixed with the water resulting to no change in color of the water.
After placing the two leaves into the beaker of boiling alcohol, the color of the alcohol changed because it extracted the green pigment. Chlorophyll is a green pigment found in the chloroplast of plants and soluble in alcohol. Iodine solution is used as color indicator to determine the presence of starch. If the color of the leaf turns to blue-back, starch is present. The places where green changed to blue-black indicate that starch was present where the chlorophyll was.
Normally, the light leaf will turn to blue/black since it stored starch, whereas, the dark leaf will not change in color. Starch is a carbohydrate consisting of large number of glucose units. Since the dark leaf was not able to undergo photosynthesis (because of the absence of light), it did not produce glucose.