SOP: McCrady's Tables

Introduction
Below is a 5-tube MPN table used to determine the most probable number of positive
organisms per 1 mL of inoculum. This is an abbreviated table as the full table would
be too large. The actual amount of sample inoculum decreases ten-fold with each
succeeding set of tubes.

In the first column are the results of the 10 mL tube, in the second column are the 1
mL tube results and in the third column the results of the 0.1 mL tubes.

McCrady's Tables for 5 Tubes

Number of Positive
First Set of Tubes - 10 Second Set of Tubes - Third Set of Tubes - MPN per 1 mL of
mL 1 mL 0.1 mL Inoculum
2 3 0 0.12

3 0 0 0.08

3 0 1 0.11

3 1 0 0.11

3 1 1 0.14

3 2 0 0.14

3 2 1 0.17

4 0 0 0.13

4 0 1 0.17

4 1 0 0.17

4 1 1 0.21

4 1 2 0.26

4 2 0 0.22

4 2 1 0.26
4 3 0 0.27

4 3 1 0.33

4 4 0 0.34