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  • Cell Culture

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    alajevar
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    This document provides information on reagents and materials used for cell isolation and culture, including Hank's balanced salt solution, trypsin, collagenase, and components of the culture medium such as DMEM F12, equine serum, chick embryo extract, insulin, L-glutamine, penicillin, and streptomycin. It also lists reagents for sample preparation and culture, such as cell density, bovine fibrinogen, Matrigel, and thrombin. Finally, it details the custom mold housing and components made of Delrin, neodymium magnets, Mylar, and silicone rods.

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    Cell culture material list

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    This document provides information on reagents and materials used for cell isolation and culture, including Hank's balanced salt solution, trypsin, collagenase, and components of the culture medium such as DMEM F12, equine serum, chick embryo extract, insulin, L-glutamine, penicillin, and streptomycin. It also lists reagents for sample preparation and culture, such as cell density, bovine fibrinogen, Matrigel, and thrombin. Finally, it details the custom mold housing and components made of Delrin, neodymium magnets, Mylar, and silicone rods.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as docx, pdf, or txt
    5 views1 page

    Cell Culture

    Uploaded by

    alajevar
    This document provides information on reagents and materials used for cell isolation and culture, including Hank's balanced salt solution, trypsin, collagenase, and components of the culture medium such as DMEM F12, equine serum, chick embryo extract, insulin, L-glutamine, penicillin, and streptomycin. It also lists reagents for sample preparation and culture, such as cell density, bovine fibrinogen, Matrigel, and thrombin. Finally, it details the custom mold housing and components made of Delrin, neodymium magnets, Mylar, and silicone rods.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as docx, pdf, or txt
    of 1

    Cell Isolation Vendor

    Hanks balanced salt solution


    (calcium and magnesium free)

    50ug/mL trypsin
    Collagenase (1500 units)
    Culture Medium Dulbeccos modified Eagles
    medium (DMEM) F12
    Thermo Fisher Scientific
    10% Equine serum Thermo Fisher Scientific
    2% chick embryo extract Gemini Bioproducts
    50ug/mL human insulin Sigma-Aldrich
    2mM L-glutamine Thermo Fisher Scientific
    20U/mL penicillin MP Biomedicals
    50ug/mL streptomycin MP Biomedicals
    63ug/mL tranexamic acid Sigma-Aldrich
    33ug/mL Aprotinin Sigma-Aldrich
    Sample Preparation and
    Culture

    4.1x10
    6
    cells/mL
    5mg/mL bovine fibrinogen Sigma-Aldrich
    100uL/mL Matrigel BD Biosciences
    15uL thrombin Sigma-Aldrich
    Custom Mold Mold Housing (Delrin) Mcmaster-carr
    2 neodymium magnets (inside
    housing)
    Applied Magnets
    Frame(cut from polyester sheet) Mylar Fralock

    Silicone Rods (VST-50)

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