TABLE - 2

EFFECT OF BAP AND L-GLUTAMIC ACID ON DIFFERENTIATION OF ERYTHRINA VARIEGATA L.

FROM STEM AND COTYLEDON DERIVED CALLI EXPOSED TO GAMMA RADIATION (5 kR)

Growth regulators and Stem derived callus Cotyledon derived callus

amino acid (mg/l) % frequency of Morphogenetic response % frequency of Morphogenetic response
growth response growth response

2.0 BAP + 1.0 2,4-D + 0.5 L-glutamic acid 68 Greening of callus 66 White friable callus
2.0 BAP + 1.0 2,4-D + 1.0 L-glutamic acid 74 Globular callus 70 Shoots
2.0 BAP + 1.0 2,4-D + 2.0 L-glutamic acid 85 Shoots + roots 88 Rhizogenesis + shoots
2.0 BAP + 1.0 2,4-D + 3.0 L-glutamic acid 80 Multiple shoots 82 Shoots
2.0 BAP + 1.0 2,4-D + 4.0 L-glutamic acid 72 Globular callus 70 Rhizogenesis
2.0 BAP 30 Callus + roots 38 Globular callus
1.0 2,4-D + 0.5 L-glutamic acid 40 Small shoot buds 45 Hand brown callus
1.0 2,4-D + 1.0 L-glutamic acid 42 Compact brown callus 45 Callus + roots
1.0 2,4-D + 2.0 L-glutamic acid 51 Callus with visible shoot buds 50 Compact brown callus
1.0 2,4-D + 3.0 L-glutamic acid 50 Friable callus 52 Browning of callus
1.0 2,4-D + 4.0 L-glutamic acid 56 Greening of callus 59 Very few shoot buds

Data scored at the end of four weeks, 70 day old callus from 10 replicate culture.