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  • LatihanMetoda Pemisahan

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    Satriani Ni Wayan
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    1. The document discusses analyzing a gel showing electrophoretic separation of DNA size standards and DNA fragments produced by cutting a plasmid with restriction enzymes A, C, and D. 2. The size of the plasmid is 4,000 bp and each restriction enzyme cuts the plasmid once at its restriction site. 3. The first step is to measure the distance migrated by each DNA standard band and plasmid fragment band and correlate it to the size in bp, to generate a standard curve.

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    1. The document discusses analyzing a gel showing electrophoretic separation of DNA size standards and DNA fragments produced by cutting a plasmid with restriction enzymes A, C, and D. 2. The size of the plasmid is 4,000 bp and each restriction enzyme cuts the plasmid once at its restriction site. 3. The first step is to measure the distance migrated by each DNA standard band and plasmid fragment band and correlate it to the size in bp, to generate a standard curve.

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    6 views2 pages

    LatihanMetoda Pemisahan

    Uploaded by

    Satriani Ni Wayan
    1. The document discusses analyzing a gel showing electrophoretic separation of DNA size standards and DNA fragments produced by cutting a plasmid with restriction enzymes A, C, and D. 2. The size of the plasmid is 4,000 bp and each restriction enzyme cuts the plasmid once at its restriction site. 3. The first step is to measure the distance migrated by each DNA standard band and plasmid fragment band and correlate it to the size in bp, to generate a standard curve.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as doc, pdf, or txt
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    Latihan Metoda Pemisahan DNA

    Given:
    1. The picture of the gel showing electrophoretic separation of DNA size
    standards and DNA fragments produced by cutting of the plasmid pX with the
    restriction enzymes A, C, dan D !ig. "#.
    $. %ize of the plasmid pX is & '"( bp base pairs# and each restriction enzyme
    A, C, D#, cuts the pX DNA at one site one time# only.
    '. The restriction site of enzyme A is ta)en as the starting point for restriction
    map of this plasmid.
    &. *ength of the DNA fragments in the bands of the DNA size standard *ane 1,
    !ig. ".#+
    ' (((, $ (((, 1 -((, 1 $((, 1 ('1, .((, /((, 0((, "((, -((, &((, '((, $((, 1(( in
    bp# . 1and of -(( bp fragments has ele2ated width is dar)er# in respect to
    neighbour bands. 1ands of short DNA fragments under -(( bp# may be wea) or
    lost from the gel.
    Lakukan hal dibawah ini!
    1. 3)ur 4ara) tempuh setiap band yang terbentu) pada 5ambar diatas dan
    Tabulasi hasilnya dengan u)uran fragmen DNA yang ditandai dengan ang)a
    6omawi 7 8 97, untu) )eperluan pembuatan )ur2a standard:
    $. ;onstru)si restriction site dari enzyme yang dipa)ai untu) memotong plasmid
    diatas:

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