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Moisture Analysis

Sample
FM 1
FM 2
FM 3

W0
41.1162
45.0038
42.1751

W1
43.1213
47.0068
44.1615

W2
42.9632
46.8461
43.9970

SBM 1
SBM 2
SBM 3

43.3011
39.9350
39.5393

45.3079
41.9350
41.5386

45.2506
41.8824
41.4786

PKM 1
PKM 2
PKM 3

41.8512
60.2981
40.7214

43.8613
62.2951
42.7436

43.6934
62. 1307
42.5419

Starch 1
Starch 2
Starch 3

41.0403
39.5626
39.4412

43.0836
41.5669
41.4535

42.8831
41.3793
41.2651

Calculate the moisture content using formula;


Moisture content (%) = {(W1-W0) (W2-W0)}

100

(W0)
Dry matter (%) = 100 Moisture (%)
Ash Analysis

Sample
FM 1
FM 2
FM 3

W0
56.6812
50.3404
60.3526

W1
58.6962
52.3430
62.3563

W2
56.9419
50.5985
60.6112

SBM 1
SBM 2
SBM 3

45.8975
43.9733
58.3250

47.8972
45.9800
60.3369

46.0480
44.1240
58.4737

PKM 1
PKM 2
PKM 3

50.7668
52.2313
52.1225

52.7701
54.2313
54.1786

51.1018
52.5706
52.4732

Percentage of ash:
Weight of ash

x 100

Weight of homogenous sample


Lipid Analysis
Sample
PKM 1
PKM 2
PKM 3

W1
2.0000
2.0000
2.0000

W2
28.0698
27.9409
27.9736

W3
28.1265
28.0253
28.0388

SBM 1
SBM 2
SBM 3

2.0000
2.0000
2.0000

26.7207
26.5955
26.5615

26.7320
26.6078
26.5815

FM 1
FM 2
FM 3

2.0000
2.0000
2.0000

26.8167
28.0689
27.9668

27.0229
28.2667
28.1755

Crude Lipid (%) =

W3 W2

X 100

W1
Fibre analysis
Sample

Weight of
Crucible
28.9292
28.8080
29.8832

W2

W3

FM 1
FM 2
FM 3

Weight of
sample
1.0124
1.0085
1.0072

29.7365
29.6212
30.7203

29.7323
29.6165
30.7150

PKM 1
PKM 2
PKM 3

1.0071
1.0045
1.0032

29.8100
29.2485
30.1800

30.8676
30.3022
31.2549

30.6107
30.0548
30.9990

SBM 1
SBM 2
SBM 3

Protein analysis
Sample
FM 1
FM 2
FM 3
SBM 1

SBM 2
SBM 3
PK M 1
PKM 2
PKM 3

Crude protein (%) was estimated using the following formula:


Total nitrogen (%) = (Titration - Blank) X N X 14.007 X 100
Sample weight (mg)
Where, 14.007= the atomic weight of nitrogen
N= the normality of HCL used in titration
Crude protein (%) = Total Nitrogen (%) X 6.25

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