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    This document summarizes preliminary research investigating the physiological mechanisms regulating senescence in sockeye salmon. The researchers found that cortisol signaling declines and molecular chaperone protein (HSP) is upregulated as salmon progress towards senescence. Genes controlling processes like olfaction, reproduction, learning, and immunity were differentially expressed between arriving and senescing salmon. Going forward, the researchers plan to compare gene expression patterns between populations with different senescence rates and take an unbiased approach like next-generation sequencing to further identify differences.

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    Gene expression associated with senescence in sockeye salmon

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    This document summarizes preliminary research investigating the physiological mechanisms regulating senescence in sockeye salmon. The researchers found that cortisol signaling declines and molecular chaperone protein (HSP) is upregulated as salmon progress towards senescence. Genes controlling processes like olfaction, reproduction, learning, and immunity were differentially expressed between arriving and senescing salmon. Going forward, the researchers plan to compare gene expression patterns between populations with different senescence rates and take an unbiased approach like next-generation sequencing to further identify differences.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    32 views17 pages

    Roberts Alaska Sen

    Uploaded by

    sr320
    This document summarizes preliminary research investigating the physiological mechanisms regulating senescence in sockeye salmon. The researchers found that cortisol signaling declines and molecular chaperone protein (HSP) is upregulated as salmon progress towards senescence. Genes controlling processes like olfaction, reproduction, learning, and immunity were differentially expressed between arriving and senescing salmon. Going forward, the researchers plan to compare gene expression patterns between populations with different senescence rates and take an unbiased approach like next-generation sequencing to further identify differences.

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    of 17

    Fishing for mechanisms regulating senescence in

    sockeye salmon
    Steven Roberts
    Tom Quinn
    Leslie Jensen
    Caroline Storer
    photo: Foist
    Salmon Senescence

    • Rapid decline to death

    • Alongside other complex physiological process

    • Rates of senescence vary within population and across populations

    • Arrival date to spawning ground correlates with rate of senescence


    (Hendry et al 2003)

    • Bear predation plays a role in driving rates


    (Carlson et al 2007)
    What physiological process regulate senescence?
    Rationale

    • Better understand how environmental change might effect senescence


    physiology

    • Determine other traits that could be impacted by selective pressures

    • Model system for studying aging in other taxa


    Preliminary Investigations
    Carlson and Quinn 2007

    Preliminary Investigations

    Hansen Creek

    Small tributary of Lake Aleknagik

    Nearly uniform top to bottom: 4m wide & 10cm deep riffles

    Annual spawning population of 3,000 – 20,000 sockeye

    Stream life annually averages below 10 days (usually 7-14)


    Preliminary Investigations

    Hansen Creek
    Approach

    • Sample fish (brain tissue)

    • Select candidate genes

    • Characterize gene expression patterns for select genes


    Preliminary Investigations
    relative gene expression

    Cortisol axis
    Preliminary Investigations
    Preliminary Investigations

    Chaperones
    relative gene expression
    Preliminary Investigations

    HSP70
    relative protein
    expression

    *Liver
    Preliminary Investigations
    relative gene expression

    olfaction repro

    learning
    immune
    aging
    Summary

    • Baseline information on physiological changes between “arriving” and


    senescent salmon

    • Cortisol signaling declines temporally

    • Molecular chaperone protein (HSP) is upregulated at senescence

    • Molecules controlling “other” primary physiological process are differentially


    expressed

    • Receptor (NMDA) associated with aging and memory in other taxa declines in
    senescence
    Future direction

    • Compare expression in populations with different rates of senescence

    • Characterize corresponding protein expression levels

    • Take a non-biased approach to identify differences (NGS, Differential Display)

    • Explore genetic (SNP) and epigenetic (DNA methylation pattern) differences

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