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    003-Lecture 6A DNA Fingerprinting

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    rabdu100
    This document is a lecture on DNA fingerprinting given by Professor Rosie Redfield of the University of British Columbia. The lecture outlines that DNA fingerprinting identifies individuals based on their unique genotypes at regions called VNTR loci, which have high levels of variation between people. DNA is a stable molecule that is left behind at crime scenes and can be reliably amplified through PCR, even from small or complex samples. Each person's DNA is unique based on millions of differences, making DNA fingerprinting a powerful forensic tool.

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    003-Lecture 6A DNA Fingerprinting

    Uploaded by

    rabdu100
    13 views11 pages
    This document is a lecture on DNA fingerprinting given by Professor Rosie Redfield of the University of British Columbia. The lecture outlines that DNA fingerprinting identifies individuals based on their unique genotypes at regions called VNTR loci, which have high levels of variation between people. DNA is a stable molecule that is left behind at crime scenes and can be reliably amplified through PCR, even from small or complex samples. Each person's DNA is unique based on millions of differences, making DNA fingerprinting a powerful forensic tool.

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    DNA Fingerprinting for Everyday.

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    003-Lecture 6A=DNA fingerprinting.pptx

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    This document is a lecture on DNA fingerprinting given by Professor Rosie Redfield of the University of British Columbia. The lecture outlines that DNA fingerprinting identifies individuals based on their unique genotypes at regions called VNTR loci, which have high levels of variation between people. DNA is a stable molecule that is left behind at crime scenes and can be reliably amplified through PCR, even from small or complex samples. Each person's DNA is unique based on millions of differences, making DNA fingerprinting a powerful forensic tool.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    13 views11 pages

    003-Lecture 6A DNA Fingerprinting

    Uploaded by

    rabdu100
    This document is a lecture on DNA fingerprinting given by Professor Rosie Redfield of the University of British Columbia. The lecture outlines that DNA fingerprinting identifies individuals based on their unique genotypes at regions called VNTR loci, which have high levels of variation between people. DNA is a stable molecule that is left behind at crime scenes and can be reliably amplified through PCR, even from small or complex samples. Each person's DNA is unique based on millions of differences, making DNA fingerprinting a powerful forensic tool.

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    © All Rights Reserved
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    of 11

    Useful

    Gene*cs
    Professor Rosie Redeld
    The University of
    Bri*sh Columbia

    This video is licensed under a Crea1ve Commons


    A4ribu1on-Share Alike 3.0 Unported license.
    The UBC crest is UBC

    ?? Lecture 6A:
    DNA ngerprin*ng
    (This lecture and the next one expand on the
    Gene*cs in the News video that was provided
    as an appe*zer for the course.)

    Outline:
    Why DNA makes good evidence
    VNTR loci have many alleles
    New combina1ons in each new person

    Learning Objec*ves:
    Explain why DNA provides valuable evidence
    2

    Why DNA makes good evidence


    We all leave DNA behind, wherever we go...
    We shed about 40,000 skin cells each minute, and each one
    contains enough of our DNA to iden1fy us.

    DNA is a very stable molecule

    Why DNA makes good evidence


    The Polymerase Chain Reac1on (*PCR) can amplify DNA reliably.
    From:
    complex or dirty mixtures
    1ny amounts

    *Heres a good short video explaining PCR:


    h4p://www.youtube.com/watch?v=2KoLnIwoZKU
    4

    Why DNA makes good evidence


    Our DNAs dier at several million places.
    Each person is unique.

    Fingerprin*ng iden*es people by their genotypes at a set


    of loci called VNTR markers
    1

    10

    11

    12

    13

    Each VNTR marker has many dierent alleles


    1

    10

    11

    12

    13

    Father

    Child

    Mother
    8

    Father

    Child
    Child 2

    Mother
    9

    What weve done


    Why DNA is good evidence:
    present at scene
    stable
    easy to test
    unique to each person
    VNTR loci are highly polymorphic
    Each child gets a dierent set

    10

    Coming up...
    Lecture 6B: Why VNTR alleles
    are prac*cal gene*c markers
    7" 9"

    7" 8"

    4" 7"

    5" 8"

    9"11"

    11

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