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Department of Biotechnology
Group 16
ABSTRACT
Aniseed or known as Pimpinella anisum L. (P. anisum) is one of the herbaceous annual plants that possesses galactopoietic effect, which
has the potential of promoting lactation in human and animals. This research aimed to determine the galactogogue effects of aniseed
extraction on milk production. P. anisum seeds were extracted with petroleum ether and ethanol respectively using soxhlet method and water
extraction method. Lactating rats (n=3) of Spraque Dawley with three pups each were treated with the extract in the amount of 500 mg/kg
body weight, while the control rats were given the same amount of distilled water. The rats were administered via intraperitoneal route for two
weeks. The milk production was evaluated by measuring the pups weight during the suckling period. Results were statistically analysed
using SPSS software. The rats treated with ethanolic extract produced higher milk production than aqueous extract compared to the control
group. Thus, both ethanolic extracted and aqueous extracted P. anisum seeds can enhance milk production in rats.
INTRODUCTION
RESULTS
METHODS
ANIMAL
Three groups of 9 lactating Sprague Dawley rats were tested in the
experiment.
EXTRACTION
P. anisum seeds extraction
DISCUSSION
Aqueous Extract
Ethanolic Extract
TREATMENT METHOD
500 mg/kg
control, P. anisum
aqueous and
ethanol extract
Administered to 3
groups
respectively
CONCLUSION
P. anisum aqueous and ethanolic extract increase milk production.
REFERENCES
Mahmood, A.; Omar, M. N.; Ngah, N. Galactagogue effects of Musa x paradisiaca flower extract on lactating rats. Asian Pacific
Journal of Tropical Medicine 2012, 5, 882-886