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  • Fructose Uptake 2-Dg Uptake: Figure 1. Effect of CSC +/-Ahr Antagonist On Hexose Uptake

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    Kenan Omurtag
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    The document examines the effect of a compound called CSC on hexose uptake and GLUT expression in spermatocytes. Figure 1A shows that CSC increases 2-DG and fructose uptake in spermatocytes and this is reduced with an AhR antagonist. Figure 1B shows GLUT expression profiles in spermatocytes and that CSC increases expression of GLUT5, GLUT8, GLUT9a and GLUT12 over 24 hours, and this is reduced by an AhR antagonist.

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    ABOG THESIS PART 2

    Original Title

    Figure1&2

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    The document examines the effect of a compound called CSC on hexose uptake and GLUT expression in spermatocytes. Figure 1A shows that CSC increases 2-DG and fructose uptake in spermatocytes and this is reduced with an AhR antagonist. Figure 1B shows GLUT expression profiles in spermatocytes and that CSC increases expression of GLUT5, GLUT8, GLUT9a and GLUT12 over 24 hours, and this is reduced by an AhR antagonist.

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    © All Rights Reserved
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    24 views2 pages

    Fructose Uptake 2-Dg Uptake: Figure 1. Effect of CSC +/-Ahr Antagonist On Hexose Uptake

    Uploaded by

    Kenan Omurtag
    The document examines the effect of a compound called CSC on hexose uptake and GLUT expression in spermatocytes. Figure 1A shows that CSC increases 2-DG and fructose uptake in spermatocytes and this is reduced with an AhR antagonist. Figure 1B shows GLUT expression profiles in spermatocytes and that CSC increases expression of GLUT5, GLUT8, GLUT9a and GLUT12 over 24 hours, and this is reduced by an AhR antagonist.

    Copyright:

    © All Rights Reserved
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    of 2

    Figure 1.

    Effect of CSC +/- AhR Antagonist on hexose uptake


    A)

    B)

    1.4
    1.2
    1
    0.8
    0.6
    0.4
    0.2
    0

    Fructose uptake
    **

    120

    **

    100
    fmol/cell/min

    fmol/cell/min

    2-DG uptake
    80
    60
    40
    20
    0

    Figure 2. GLUT Expression profile of Spermatocytes


    GLUT5
    GLUT8
    GLUT9a
    GLUT12

    50 kDa

    A)

    50 kDa
    75 kDa
    50 kDa

    actin

    40 kDa

    CSC
    (-)
    Antagonist (-)

    18 hr
    (-)

    24 hr (-) 18 hr
    (-) (+) (+)

    24 hr
    (+)

    B)
    GLUT Expression in Spermatocytes
    (40 g/mL CSC)
    2

    NS

    NS

    1.8
    P=0.08

    1.6

    P=0.07

    Fold Change

    1.4
    1.2

    DMSO
    DMSO + Antagonist

    CSC +24 hr

    0.8

    CSC + Antagonist 24 hr

    0.6
    0.4
    0.2
    0

    GLUT5

    GLUT8

    GLUT9A

    GLUT12

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