Design qPCR for different regions with low-normal-high

coverage

1) Low coverage:
>PDPK1 16dna:chromosomechromosome:GRCh37:16:2616226:2616512:1
TAGCCCTGGTTGTCAGCCTGTAAGGATCATATACATTCATTCGTTCATTTTGTTTTCAGCAAAATTTAG
AGTACGTAATAATGTTGCTGTTTTAAGTATTTCTTCTCAAATGTGAAAATCTGCTTTTACAGGGACCTT
AAACCGGAAAACATTTTGTTAAATGAAGATATGCACATCCAGATCACAGATTTTGGAACAGCAAAAGTC
TTATCCCCAGAGAGCAAACAAGGTGTGTGAGTTTTATTTCTAGCAGAGCCTGGCTCTGTGCTTCAGATG
GAAAGCGACTT
F:GTTGTCAGCCTGTAAGGA
R:TAAAACTCACACACCTTGTT
>HOOK3 8dna:chromosomechromosome:GRCh37:8:42849470:42849671:1
TTATTATTTCTTCTACTAATTTTGGGTTTGGTTTGCTCTTCCTTTTCTAGTTCATTCAGTGGCATCGTT
AGGTTGTTTATTTGAAGGTTTTCTCTTTTTTGATGTAGGTACTTGTGCCCATAAATTTCCCTCTTACTA
CGGCTTTTGCTGTATCCCATAGATTTCGGTATGTTGTGTTTCCATTATCATTTGTTTCAATAAA
F:TCATTCAGTGGCATCGT
R:AACACAACATACCGAAATCTA
>WIF1 12dna:chromosomechromosome:GRCh37:12:65477472:65477672:1
CTGGTTCATTATCTTCCAAAATCAGCTTCCCCCACTTGATTTCTCTTTTCTTTTCTTTCCTTTCCTTTC
CTCTTTTCCTTTTCCCTTTCCCTTTCCTTTCCTTTCCTTTCCTTTCCTTTCCTTTCCTCTCCTCTTTTC
TCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTATTTGCACCTCACTCTTGCCCAGGCTGGGATG
F:AGCTTCCCCCACTTGA
R:CAAGAGTGAGGTGCAAA
>PBX11dna:chromosomechromosome:GRCh37:1:164524735:164524971:1
CCTCCTGAGTAGCTGGGACTACAGGATAGTGCCACCATGCCCGGCTCATTTTTAATTTTT
TTTTTTTTTTTTTTTTTTTTTTACAGAGACAGGGTCTCACTATGTTGCCAGGCTGTTTTC
GAACTCCTGGGCTCAAGCAATCCTCCTATCTTGGCCTCCCAAACTGCTGGGATTACAAGT
GTGAGTCACTGCGCCTGGCCTGAAGATTCTATTTCACCTATGGTACTTCCTCCTCTT
F:GGACTACAGGATAGTGCCA
R:GGCGCAGTGACTCACA
>SSX2 Xdna:chromosomechromosome:GRCh37:X:52727750:52727996:1
GCATTTATCCTTGTGTTACAAACAATCCATTTACACTTTTAGTTTTTTTTTTAATGTACA
ATTAAGTTATTATTGACTATAATCACCCTGTTGTGTGTAATTGTTTTGGGGGTACCATGA
ACTGCACCCATAGAAGTTGACAAACTGAATCGACCAATGTTGTGTGTGTTCTGACTGCTC
CACCGATGAGCTGTTCCGCGTCTCTCTTCCTTTTCTTGGGCCTCCCTATTTCCTGAGACACAGCAAT
F:GTTACAAACAATCCATTTACA
R:AGAGAGACGCGGAACA
>SSX2 Xdna:chromosomechromosome:GRCh37:X:52734092:52734319:1
CTTTCTGCAGCCTAAGAAGTAGCCAAAGCAGGAAAAGGGATGCTCATGTGTCCCCAGACT
TGTCTGTTCCCAGAACTTTCTGTTACCTAGTTTAGTCATAGCCTCATACTTTCTCTTCAT
ATACACATAGAAGATTTTCTCCGAGGCTTTCATCTTTTCCCACTCTTCCTTAGAGAAGTA
TTTGGCAATATCATCGAAGGCCTACAAAAAAAAAAAAAAGGAATTATG
F:GAAAAGGGATGCTCATGT
R:TGTAGGCCTTCGATGATA
>SDHC 1dna:chromosomechromosome:GRCh37:1:161296355:161296573:1
ATATATATATATATATTAGGTCTTTAAGGTTTTATTTTATTTTATTTTAATTTTTAATTT
TTTTTTTTTCTTTTTTTTTGAGACAGAGTCTCGTTCTGTCGACCAGGCTGGAGTGTAGTG
GCGTGATCTGGGCTCACTGCAACCTCCACGTCCTGAGTTCAAGTGATTATCCTGCCTCAG
CCTCCTAAGTAGCCGGAATTACAGGTGTGCACCACCACG
F:GACCAGGCTGGAGTGTA
R:CCTGTAATTCCGGCTACT
>BRCA2 13dna:chromosomechromosome:GRCh37:13:32949376:32949576:1
GCTCCTTATCATTTAAGTTTTATTGTGACATTGCAGTAATTCAGTCACATCTTCAGGCTT

TACTTCTAATTCTAGTTCTCTTGCTATTTTCACCATATATGCAGTTACTTCCTCCACTGA
AGTCTTGAACCCCCCAAAGTCATCCATGAGGGTTGGAATCAACTTCTGGTAAACTTGTTA
ATGTTGATATTTTGACTTCCC
F:TGCAGTAATTCAGTCACATC
R:CAACATTAACAAGTTTACCAGA
>SLC30A4 15dna:chromosomechromosome:GRCh37:15:45807885:45808105:1
ATAGAAATTTCAACTATGTTTTTATTGTGCAGAAGCTTTTTTCGTTACCCTGAATTTCTT
TTCTTTTTTTTTTTTCTTTGAGATAGAGTCTCACTCTGTCACCCAGGCTGGAGAGCAATT
GCACAAACACTTGGCTCACTGCAGCCTCAACTTCTTGTCCTCAAGTGATCCTCCCACCTT
AGCCTCCTAAGTAGCTGGGACCACAGGTAAGCACCACCATG
F:ATGTTTTTATTGTGCAGAAG
R:GAGGATCACTTGAGGACA
>PDPK1 16dna:chromosomechromosome:GRCh37:16:2615428:2615751:1
AGCCAGGGAGGTTGAGGCTGCAGTGAGCTATGATTGTGCCCCTGCACTCCAGTCTGGGCA
ACGGAGTAAACCTGCTCCAAAACGAAACAAGAGATTTTTACACTTCCTTAACTGCACGAT
ATTCAGATTTCGGCCTTAGTTATGCCAAAAATGGAGAACTACTTAAATATATTCGCAAAA
TCGGTTCATTCGATGAGACCTGTACCCGATTTTACACGGCTGAGATTGTGTCTGCTTTAG
AGTACTTGCACGGCAAGGGCATCATTCACAGGTAACCGCGGGGGTGGCTGGGTGGGTTTG
CAGGACGTCAGTTTGATGATCAGG
F:GTCTGGGCAACGGAGTA
R:GCGGTTACCTGTGAATGA
>SSX2 Xdna:chromosomechromosome:GRCh37:X:52736020:52736296:1
CTGAAAAAGTTAGAAACTGACAGGAAAGATTCGAGATGGCGGCCTGCCTCATATACACTC
CTTATTAAAACTAGATAGCAAATGCACCGCGGAGGAGGGGAGGGGTAGGAAGAATGGAAA
AAGAAAATCGGCGTATGCTTACTCTGATTTTGGAAGAATCCAAAGAGAAAATCAGACCGT
GCGTACTCTGAGTATGGAAGAATCGAAAGAGAGAGAAAGTCAGAGCATGCGTACTCTGAA
CTTAAAGTAGCCAATCCCAGGGGATGCTTTAGGCGGG
>PDPK1 16dna:chromosomechromosome:GRCh37:16:2631470:2631752:1
CCGTGCCCAGGTGGGGGGCTCTCGGCGTGCGGGGCCGGGGTGCGTTTGCCGTGCTGCGAG
TCCGGGGCGCTCATCCCTGCGTGTGGAGGCTTCTGGGACCCTGCCTGGGATGACTTTTTT
TTTCTTAAATTTTTACAGAAACGAGTATCTTATATTTCAGAAGATCATTAAGTTGGAATA
TGACTTTCCAGAAAAATTCTTCCCTAAGGCAAGAGACCTCGTGGAGAAACTTTTGGTAAA
TATCGTGAATTTCCTTTTAGAAAGGTGATCCGGGACACCCTGA
>SSX4 Xdna:chromosomechromosome:GRCh37:X:48248652:48248965:1
AATTTAGGCCTGTGTAACTCTCCCAGTCTTGTTGAGAGCATTGAATAAGCTAATGCACGT
GAAGACCCTTTGTAAGCTCTAAAGCACTGTAGAAATGTCACTGATACTGTTTATCTGCGA
CCTTCACATTGTAAAGATCATGCCCAAGAAGCCAGCAGAGGAAGAAAATGGTTTGAAGGA
AGTGCCAGAGGCATCTGGCCCACAAAATGATGGGAAACAGCTGTGCCCCCCGGGAAATCC
AAGTACCTTGGAGAAGATTAACAAGACATCTGGTAAGAGGAAAGAATTCGGGAACAACCC
CTCTGGCTTCCCTG
>SSX4 Xdna:chromosomechromosome:GRCh37:X:48246593:48246873:1
CAACCATGACTAAACATAATTCAGAAGCAAATCTCAAATAACTCCTCAACAATGAGTGGA
CTCACAACCTCTGCTGCAGAATGCCCTCATGCGACAGAAGTCTCTCTAGAGTTTGGAAAT
CTTTACCAACAAAGAAAAATTCTGATGTATTCTCTTTCAGTTGAACGTCCTCAGATGACT
TTCGGCAGCCTCCAGAGAATCTTCCCGAAGGTGAGTGTCTCTCAAATCTAAAGGACCAGA
GAACGTTTGTCCCTGCACGGATGCGAACACTGATAAGAGTG
>CHEK2 22dna:chromosomechromosome:GRCh37:22:29122147:29122364:1
CGCCACTGCACTCCAGCCTGGACGACAAAGCAAGACTCTGTCTGAAAAAAAACAAAAAAC
AATTAACCAGATGTGGTGGTGCATGCCTGTAGTCCAGCTACTCAGGAGGCTGAAGGAGGA
GGATCACTTGAGCAAGATAAGCTGATGCTACAATGGGCTGTGATCCTGCCACTGTACTCC
AACCTGGGCAACAGAGCAAGACCCTGTCTCAAAAAAAT
>PDPK1 16dna:chromosomechromosome:GRCh37:16:2631148:2631429:1
CCCACCCTCACACCCAACTGGTCTGAAAGGATAGAAGCGCATTAGAGGTGAAACTGGGGC
CTTGGAGACAGAGTGGGAAATGGACTGGGCTTGTCGCTTGTATTTTACACATGTCTTTGT
TTGTTTGTTTGTTTCCCCTACCTTAAAGTTCAGACCTTTGGGCTCTTGGATGCATAATAT
ACCAGCTTGTGGCAGGACTCCCACCATTCCGAGCTGGGTAAGGAGACGGCGTTGCCTGGG
CCTTGGAGCCTGTTGGGGTGCACCCCACCGGCCGGCAGTCCC

>SSX4 Xdna:chromosomechromosome:GRCh37:X:48251178:48251430:1
GGGAATGTGAAAAACTGGATAAAGAAGGTCCGGGGCACTTGGGAGGGGAGAGGCATCTCC
TTCTTTTGAGAAAACAGAGCCTAACGCTCTCCAATCTACCCAACCCTCATTTTCCAACTC
TTCCCCATCATAGGACCCAAAAGGGGGAAACATGCCTGGACCCACAGACTGCGTGAGAGA
AAGCAGCTGGTGGTTTATGAAGAGATCAGCGACCCTGAGGAAGATGACGAGTAACTCCGT
AAGTGAACTTTCG
>ZNF331 19dna:chromosomechromosome:GRCh37:19:54026432:54026784:1
GGACATTTAAACTCTACTCTCTTAGCAATTTTCAAATATACTATATTTTTTTTTTATTTT
TTTTTTCTTTTGCCCTTGTCCCCCAGGCTGGAGTGCAATGGCATGATCTCAGCTCACTGG
AACCTCTGCGTCCTGGGTTGCAGCGATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGAATT
ATAGGCACCCACCACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCAT
CATGTTGGCCAGGCTGGTCTCAAACTCCTGATTTCAAGTTATCTGCCCGCCTCAGCCTCC
CAAAGTCCTGGGATTACAGGTGTGAGCCACCATGCCCTGCCTTTGTCCCAATT
>ELL 19dna:chromosomechromosome:GRCh37:19:18582040:18582240:1
CCTAGCATGGTAGTGTACACCAGTAGTCCCAACTACTTGAGAAGCTGAGGTGGGAGGATC
CCTTGAGCCCAAGAGTTCAAAGCTGAAGTAAGCTATGGTTGTGCCACTGCACTCCAGCCT
CGGCAACAGAGAGACTCATCTCTTAAAAAACAAAACAAGGCTGGGCGTGGTGGCTCACCT
GTAATCCCAGCACTTTGGGAG
>SSX2 Xdna:chromosomechromosome:GRCh37:X:52726918:52727153:1
AGTAGAAAATTGTAAGAAGGGAATGATTTGGGGAGCAAGTGACAGATGGGATACCAGTAT
CATAACAGAATAGCACGTCTGCAGGGATGTGGGGGATGAGTGGAAGGTTCTCTTACGGAG
TTACTCGTCATCTTCCTCAGGGTCGCTGATCTCTTCATAAATCACCAGCTGTTTTCTCTC
ACGCAGTCTGTGGGTCCAGGCATGTTCCCCCCTTTTGGGTCCTGTGATGGAGAATA
>SSX2 Xdna:chromosomechromosome:GRCh37:X:52731498:52731793:1
ACATCAGGTGTTGTGATAGACATGGGGAGAAGAAGGCAGTGAGGGCATTTTTGATATTCT
CCCACTCTTACCAGTGTTCGCATCCGTGGAGGGACAAAGGTTCTCTGGTCCTTTAGATCT
GAGAGACACTCACCTTCGGGGAGATTCCCTGGAGCCTGCCGAAAGTCATCTGAGGACGTT
CAACTGAAAGAGAATACATCAGAATTTTTCTTTGTTGGTAAAGGTTTCCAAACTCTAGAG
AGACTTCTGTCGCATCAGGGTATTCTGCAGCAGAGGGTTATGAGTCCACTCATTGT
>SSX2 Xdna:chromosomechromosome:GRCh37:X:52733459:52733692:1
CAAATGCCTGAGGATCTTTCTCAGGTAGCTGAGCTGAAAAGCAGCTGGGCTTGAGGAGAC
CCTTTCCAGCCCCTTCCCATCTACTCACCCTGATTCCCACGGTTAGGGTCATTATCCAAA
TCATTCCCCTGGAAGTCTTCGGCCCGTTTATTACACATGAAAGGTGGGAGGGTGGCCTTG
AAACCTAGAAAGAAGCAAAATGTTTATTCCTTAAGAGACAAGCTTGGGCCTGGT
>SSX2 Xdna:chromosomechromosome:GRCh37:X:52729362:52729674:1
CTGGAGCTGGGCGAGCTCCTCAGCCCAGTCTGGACCCAGGGTTATCTGGGATCCATGCCA
CACACCCAGTCCATATACCTGAACATCGCCAGGGAAGCCAGAGGGATTGTTCCTGAATTG
CTTCCTCTTACCAGATCTCTCGTGAATCTTCTCAGAGGTAGTTGGTTTTCCCGGGGGGCA
CAGCTCTTTCCCATCATTTTGTGGGCCAGATGCTTCTGGCACTTCCTCCGAATCATTTCC
TTCCTCTGCTGGCTTCTTGGGCATGATCTTTATAATGTGAAGATCACAGATAAATAGTAT
CAGTGACATATCT
>TCF4 18dna:chromosomechromosome:GRCh37:18:53298438:53298639:1
ACCCAGCTAATTTCTGTGTTTTTAGTAGAGACAGGATTTCATCATGTTGGCCAGGCTGTC
TTGAACACCTGACCTCAGGTGATCCACCTACCTTGGCCTCCCAAAGTGCTGGGATTACAG
GTGTGAGCCACCATGCCCGTCCAAGAGATAATGTTCTTAGATTGGTGTTTACAAAACATT
TGCTGCAGGGTGGAGAAAAGAT
>FCGR2B 1dna:chromosomechromosome:GRCh37:1:161639867:161640148:1
GGAGTTCTCAGTCCTTTTAAAAGTGCAGCTCATCCAGGCAGTCCCAGGGGATATGGCCAC
GAGGGCTGTGCTGTAGGTGAAGGGCGCTCTGGGCCTGGGCAGTCTCTGGGCCAAAGTGGG
CAGGTGTCAGTTCAGACACTCCACTGTGGGCTGATCTTTGCTTTCTGTCTTACAGCTCCT
GTTGCTGGGACACCTGGTAAGTACTCCACCCACAGCCTTTCCTTCCCTCGGGTCCCCTTT
TCCTCTCCTCCCAGTGCCCTGTCTGGAGGTGCTCTAAATGCA
>SSX2 Xdna:chromosomechromosome:GRCh37:X:52734613:52734875:1
AGATCTCCCCCGAGACCCTGCTCCTTGTCTCCAGTATCTCTGTCCTCCCCTCCTCAGAAA
CCGGGTCACCCCACACTGTCCCCTGGGCCACTACTCTGCTCCCTCCAGGTCACCTCACCT
TTTGGATCTTCTCTGGTATTTGAGCACCAACCGTGGGTCTCCTTGCAAAGGCGTCGTCTC

CGTTCATGGCACCGGGAGCAGTCTGACCTGCAAGAGAAATAGCCTGAGTCTTTCCAGCCA
CAGCAGCTTTGATCCTGTGGAGG
>CHEK2 22dna:chromosomechromosome:GRCh37:22:29117420:29117634:1
GCCTGCCATCATGCCCGGCTAATTTTTGTATTTTTAATAGAGACAGAGTTTCACCACGTT
GACCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCTGCCAGCCTCGGCCTCCCAAAGT
GCTGGGATTAAAGACGTGAGCCACCGCGCCCCGCCGTATTTTTGAAAATCAGCTGAGAGA
GTAGATTTTAAGTATTTTCTCTATTAAAAAAAAAA
>RP11-322M19.1 10dna:chromosome
chromosome:GRCh37:10:88990546:88990859:1
ACATGGGGGAGAACAGGACAGGGACAGATGGCAGGACAGGTGTGGGGAGGACAGGGGCCA
GGTGTTGGGACCAGGTGGGCTTGGGATGAAGGGTGGGCTTATAGACTGAGACTGACTGCA
CTGGTTTACAGCCCAGTTCTCCGATCCCTGGCCCGGCGGAAGCCCACCATGACCCTGGAG
GAGGGACTGTGGCGGGCCATGCGGGAATGGCAGCACACGAGCAACTTTGACCGGATGATC
TTCTACGAGATGGCGGAAAAGTGAGTCTGGGGTCCTGGGAGCGGGGCCCGCGTGGCAGGG
TGAGAGTGAATGAC
>SET 9dna:chromosomechromosome:GRCh37:9:131452105:131452387:1
CTGGGGGCCTGGGTGGCCCGCGCCGCGGCGGCCGCGCTGCGAGGCGGGGGCGCTGCGGCC
TGCTCGGCGCGGGCGGAGGAGACCCCCCCTTCCTCTCCCCCCTCCCTCGCTCTCCTCCCC
CTCCCTCCCTCCCGAGAAGCCTCTCTTTATTGTGCTCCGCCATGATGCCTCGCTCCCATC
AGCCGCCGCCGCCGCCACATGGTGCGGCCGGACGCGGCCCCGCGCCCGACCTCCCGCGCG
GTTCCGCTTCGCGCCCGGCCCGCGGCGGCGCCCCCGGCACCCC
>SOCS1 16dna:chromosomechromosome:GRCh37:16:11349805:11350083:1
TGGGGCCGGCGAGGGTCAGGGGCATCGCGGCCGCGACCCCATTCTGCAGCCCCCGAGGCT
CGCCCGACTCCTGGCTGCCCTGGACTCCCCTCCCTCCTCCCTCCCGCCTCCTCGCCCAGG
GCCCGGCTCACCTGGCGGCGGGGCGCGGGACGCCGCGGGCGGGACGGCGGGGGGCTCCGG
GGCGCTCCGGGGCGGCTCTCGCGCATGCTCCGGGGCCAGGAGCCGTGCAGCTGCCACGGC
CGCAGCTCGCTCTGTTCGGCGCCCGCCCCTGCGCCAGTC
>CCNE1 19dna:chromosomechromosome:GRCh37:19:30302770:30303102:1
GGGGCGGGACGGGCTCTGGGTCCCGCGCGGCCGCTGAGGGGCTGGGAGCCGCGGCGGGGC
GGTGCGAGGGCGGGCCGGGGCCGGTTCCGCGCGCAGGGATTTTAAATGTCCCGCTCTGAG
CCGGGCGCAGGAGCAGCCGGCGCGGCCGCCAGCGCGGTGTAGGGGGCAGGCGCGGATCCC
GCCACCGCCGCGCGCTCGGCCCGCCGACTCCCGGCGCCGCCGCCGCCACTGCCGTCGCCG
CCGCCGCCTGCCGGGACTGGAGCGCGCCGTCCGCCGCGGACAAGACCCTGGTGAGTCTAC
TGCCGCGGGCTGCGGACAGGGACTCCTGGGGCC
>DNMT3A 2dna:chromosomechromosome:GRCh37:2:25564543:25564824:1
CCCGGCCCCACGGGCGCCCGGCGCCGAGTTGCAGGGGTCCGGGAGCGTGCCCCGAGCCGG
GCACTGCCAGCCGCGGCGCCCCCCGCCCGGCTCCCCGGCTCCCCGCCGCCTCCGGGCCGG
CCTTCCGGGCCGCCAGCAGCGGCGCTCATTACCGTATGGCCGGTGGGGTCGGGCCGGCCC
GGCTGCGCGCCCTGGTGCCGCGGCGCCGCGTCCCGGCTCGTCCTCTGCTCTCGCCGCCGC
CGCCGCCCGCGCGCCCTCCCTCCCTCCCGGCCCGCCTGCCTG
>TCF3 19dna:chromosomechromosome:GRCh37:19:1652127:1652406:1
CGCGCGAAGTTGGAGAACAATGACTCCCGGGCCGCGGGGCGCCCCCCGACACCGCCCCCG
CCCCAACTTCCTCCGCGCCCCCCCCCAACAAGCGCGCGCGGGGCGGGCCGGGGTTCCCTG
GGCACAAAGCACGGGGGTACCGGGCGCGCCCCCCGCCCCCCGCCGGGCTCACCTGCTGGG
CGCGGCCGGGCACGCGGCGCGTGGGGGGGGGCGGCGGCATGAAGCGGGGGGGCCCCCCCC
CGGACAAAGGTGCGTCGCGGCCGGGCCCCCGAGGGTCGCG
>LRP5 11dna:chromosomechromosome:GRCh37:11:68079946:68080288:1
TCCGGGGGGCGCCCGCGGGAGGGCGCGGCCGGTGCGGGGGGCGCGGGTGGCGGCGGCGGG
GGGCGGAGACGCGGCGCGGCTTCCGCTCCCGCGCGCCCAGCTCCCTCCTCCCCGTCGTCC
TGGTCCGCGGCGCCCGAGGGGGGAGGCGGAGGCGCCGGGAGCCGCGCGAGGAGCCGCCGC
CGCCGCGCCATGGAGCCCGAGTGAGCGCGGCGCGGGCCCGTCCGGCCGCCGGACAACATG
GAGGCAGCGCCGCCCGGGCCGCCGTGGCCGCTGCTGCTGCTGCTGCTGCTGCTGCTGGCG
CTGTGCGGCTGCCCGGCCCCCGCCGCGGGTAGGTGGGCGCAGG
>FGFR3 4dna:chromosomechromosome:GRCh37:4:1794903:1795230:1
AGGGGGCGTGCCCTGCGCCCCCGCGAGCCGGGCGGGGACCGGGCGGGAGCGGGGCGGGGC
CGGGGCGGGGCGCGGACCGTCCCCCACTGGCTGCGGCGCGCGGGGCAGCCCAGGCTCAGT
GCGCGGTGGCGGCGGCGTCGCGGGCAGCTGGCGCCGCGCGGTCCTGCTCTGCCGGTCGCA

CGGACGCACCGGCGGGCCGCCGGCCGGAGGGACGGGGCGGGAGCTGGGCCCGCGGACAGC
GAGCCGGAGCGGGAGCCGCGCGTAGCGAGCCGGGCTCCGGCGCTCGCCAGGTCCGTGCTT
GGGGCCGGGCAGGCTCGCGGAGGGGTCC
>PPARG 3dna:chromosomechromosome:GRCh37:3:12329273:12329564:1
TCCCAGTCGCGCGCCGCCGCCCCCGCCCCCGCCCCCGCCCCCGCCCCCACCCCCACCCCC
ACCCCCACCCCCAGCCGGCGCCCGCGCCCGCCCCCGCGCCGGGCCCGGCTCGGCCCGACC
CGGCTCCGCCGCGGGCAGGCGGGGCCCAGCGCACTCGGAGCCCGAGCCCGAGCCGCAGCC
GCCGCCTGGGGCGCTTGGGTCGGCCTCGAGGACACCGGAGAGGGGCGCCACGCCGCCGTG
GCCGCAGGTCAGAGTACGGGTGCCCGCGGCGCTCGGGAACCGGCTGCTGGCT
>WHSC1 4dna:chromosomechromosome:GRCh37:4:1873035:1873313:1
CGCGCACTTTTCCCGCGGCTTCTGCGAGGCGGCGGCGGCCCCGGCTGCGGCGGCGGCGGC
GGGCGGGAGGCGCGGGGGCGGGGTCGGCGCCGCGCGCGAGAGCCTCGGCCTGGCCGCGCT
GCGCCCGCCGCCGCCGCCGCCCCCTCCCCGCCTGGGCCCTACCGCCGCACGGCCCCGGCC
CCCTCCCAGCCTGCCGCTCCGGAGAGCCGCCCGCCGAGGATGCGACGCACCGCAGGTCAC
TGGGGCGCCCGCCCAACAGTCGCGGGCCGCCAACCGCCG
>MLLT1 19dna:chromosomechromosome:GRCh37:19:6279649:6279974:1
CCGAGGAGCGGCGGGCGCGCACAAAGCGGGCGGGCGGGCGCGGAGCGGGGAGGCCGGGAC
CGGGGAGGGCCGGGCGGGCCGGCGGGCGGGAGGGGGCCGGGCGGGCTGCGCAGGGGCGGC
GCGGCCGCTCCTCACCTGATTGTCCATGGCTGGCGCCCCGCCCCGCCCCCGGGCCCCGCG
TCGCTCGCCGCCGCCGCCGCCGCCGCCGCCGCTCAACGCCGCCCCGCCGCCCTCATTGTC
TGTCAAGCGCCGCCGCCGCCGCCGCCGCCGCCGATCCCGGCCGCCCGCTCGCCCGCCAGC
CCCGCGGAACCGGAAACCACCGCCAG
>RAD51B 14dna:chromosomechromosome:GRCh37:14:68536189:68536390:1
GTTCCCGTTTTTGAGTCCATGTGTACTGAATATTTAGCCCCCACTTATAAGTGAGAACAT
GCAGAACCAAATACTGTTTTTCTGCATTATATCTTAGGATTATGACCACCAGCTCCATCC
ATGTTCCTGCAAAATGACATGATCTCACTTTTTTAATAGCTGCATAGTATTCCATGGTAT
GTATGTACCACATTTTCTTTGT
>CASC5 15dna:chromosomechromosome:GRCh37:15:40902900:40903100:1
AGTATACCTTGAGTCTTGGGAGTAGAGAGTAGAGTGGATAGTAAGTAAATTTATTTATTT
ATTTATTTTGAGACGGATTTTCACTGTTGTTCCCCAGGCTGCAGTGCAATGGTGCCATCT
CAGCTGCAACTTCTGCCTCCTGGGTTTAAGCGATTCTCCTGCCTCAGCCTCCCAGGTAGC
CGGGATTACAGGCATGCACCA
>BRCA213dna:chromosomechromosome:GRCh37:13:32970042:32970268:1
TTCCCATACAGCATATCTACTGTTTACACCCCACATTTTCTTTTTTTCTTTCTTTCTTTT
TTTTTTTTTTTTTTTTTTTTTTTTAGAGACAGAGTCTTGCTTTGTCACCCCCAGGCTGGA
GTACAATAGCACAATCTCGGATCACTGCAGTGTCTGCCTCCTGGGCTCAAGCGATTCTCG
TGCCTTAGCCTCCCAAGTAGCTGGGACTACAGGTGCGTGCCACCACG
>AKT31dna:chromosomechromosome:GRCh37:1:244013201:244013477:1
CAACCCAGAAGCCCCCGCCTCACCCCACCCCGCCGCCATCCCCCGCCTGAGGGAGGCAGG
GAGGGCGGACCGGGGCCCGGGGCCCGGAGGGAGTGGAGGCCAGGCGGGGAGGGGGCTAGA
GTTGGGGGCGGTGGCTGTTACCTGCAACGGCGGCGGCGGCGGTGGCGGCCCCGCAGCTGC
TCGGGCGGCGGCGGAGGATGGAGCCGGGGGGGGGCGGGGGGAGGAGAGGGGGCGACTCGG
GGGTCCCCCCTCCCTCCTCCTCCCCTCCTGTCCTCCC
>TP5317dna:chromosomechromosome:GRCh37:17:7565011:7565347:1
CACCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGGGAGCCACCGTGCCCGGCCTC
CAGTATTTTGTTTATTTATTTTTTTTGAGACAGAGTCTCACTCTGTTGCACAGGCTGGAG
TGCAGTGGCACAATCTCTGCTCACTGCAACCTCCTCCTCCCTGGTTAAGAGATCCTCCTG
CCTCAGCCCCCTTAGTAGCTGGGATTACAGGCGTGGGCCACCACTGCCAGGCTAATTTTT
GTATTTTTAGTAGAGATGGGATTTCGCTATGTTGGCCAGGCTGTCTTGAACTCCTGACCT
CAGGTGATCCACCTGCCTTGGCCTAAAGAGAGAGAGA
>GNAS20dna:chromosomechromosome:GRCh37:20:57466905:57467279:1
CGCCTGCTGCTGCTGGGTAAGGGCGGGCGGGGGGCGCCGGCCCCGGCCCGGGGGCCCTCG
AAGGGCGCCCCGCAGGCCGCGCGCGCCGAGCCCGCCCCCCGCCCCGGGCGCGCGCTCCCG
AGCCCCCCGCCCGTTCGCGGGCTCTGTCTGTGGGGGGCGAGGCCGGAAGGGGGACCCAGG
GGCGCGGATTCGGCCGGGCGGGGGCTCAAAAACGGGGCGGGGGGCCGTGGCGACGCGGGC
GCGGGTCCCCCTCCCCCGGCCTGCCCGCTCAGTGTCTCTCTCTTGCTCTCGCTCTCGCTC

TCCCCCTCTTTCTCTCTTTCTCTCTTTTTCCGCGAGGCCTACACGACGCCAGGGGTTTGG
GTGCGTGTTGGGGAG
>KRAS12dna:chromosomechromosome:GRCh37:12:25403550:25403917:1
CCGACCCCGCGCCCGCGCCCCCCACCCGCCCCTCCGGGGACCCCTAATTCATTCACTCGC
CGCCGGCCCCGCCCGGCGCCGGCAAAGAGGGTCGGGACCCGGGCAGGGGCCCAGGAGGGG
TGGTCCGCTCCGTACCTCTCTCCCGCACCTGGGAGCCGCTGAGCCTCTGGCCCCGCCGCC
GCCTTCAGTGCCTGCGCCGCGCTCGCTCCCAGTCCGAAATGGCGGGGGCCGGGAGTACTG
GCCGAGCCGCCGCCACCTTCGCCGCCGCCACTGCCGCCGCCGCTGCTGCCTCCGCCGCCG
CGGCCGCCGCCTAGGAAAATCGAGCTCCGAGCACACCGATGAGTTCGGGGCCGGGCGGCC
GCAGAGGG
>BRAF7dna:chromosomechromosome:GRCh37:7:140624234:140624608:1
CCCAGCCCGCGGAGCTGGCCCGAGAAGGTGGCTGAGGGCATCAAGCCCCCACCGCCGCCT
CTTTCCAAAATAAACACCAGCCAGCCGCCGAGCCCGGAGTCGGGAGGGCGGCAGGGTGGC
GCCAGCACTCACCTCCTCCGGAATGGCAGGGTCCGCAGCCGAAGAGGCCGCGGCGCCGGC
GCCGGCGCCGGCCTCGGGCTCCATGTCCCCGTTGAACAGAGCCTGGCCCGGCTCCGCGCC
GCCACCACCGCCACCGCTCAGCGCCGCCATCTTATAACCGAGAGCCGGGGCCCGAGCGGC
CGCTGTCGGGCGGGGAGGGGGAAGGGAGGCGGAGAGCTGGGGGAGGCGGAGGCGGAGGCG
GAGGCGGAGGAGCGG

2) Normal coverage:
>PBX11dna:chromosomechromosome:GRCh37:1:164532328:164532610:1
TAAGTCATGAAAACAACTGAAGCATGCCACAGAGTTAGGGTTGGGTATATAATTTTGTCT
AAATGTTCAAATGTTTTCACCCTGTGCATTATCGGCAGTTTGATCTTGAGAGTCCACCTA
AGCTATCATGTTGTTTCTTTCTTGCAGAAAACATGCTTTAAACTGCCACAGAATGAAGCC
TGCCTTGTTTAATGTGTTGTGTGAAATCAAAGAAAAAACAGGTAGGAATGAGATTCCAAC
ATTTTAGCATTTTCTTTGGCCAATTAAATCACTTAACCAGCAG
>BRCA213dna:chromosomechromosome:GRCh37:13:32903421:32903704:1
TGACCTTTCTAATTACTATACTTAAGTACTTGAATCAATTCATTTTGTTTCAAATGTGTC
ATGTAATCAAATAGTAGATGTGCTTTTTGATGTCTGACAAAAAATAAGTTTTTGCATTCT
AGTGATAATATACAATACACATAAATTTTTATCTTACAGTCAGAAATGAAGAAGCATCTG
AAACTGTATTTCCTCATGATACTACTGCTGTAAGTAAATATGACATTGATTAGACTGTTG
AAATTGCTAACAATTTTGGAATGCCTTGTTAAATTATTTATCTT
>PPARG3dna:chromosomechromosome:GRCh37:3:12353730:12354012:1
CTCAGGGAAAGCAAACATTCAGAATGAAATCCAATAGAGAAGGTAAATTTATTTGGGCAT
GTACATTTTGGCAGCCTAGGCTGTGTACATGTGTACACATTCTGAACATGTGTGTATATT
GAAAATCTTGTCTCTTTTTTATTGTTAAGATTTGAAAGAAGCCAACACTAAACCACAAAT
ATACAACAAGGCCATTTTCTCAAACGAGAGTCAGCCTTTAACGGTAAGTAAAATCAGAAT
TTATACTGCATTTGTATTGAAAAGTATCCCTTTTAAAGAATAT
>KRAS12dna:chromosomechromosome:GRCh37:12:25380057:25380390:1
TTATTTAAAAATTTTTATTAAATATTATATGCATGGCATTAGCAAAGACTCAAAAAATAA
AAACTATAATTACTCCTTAATGTCAGCTTATTATATTCAATTTAAACCCACCTATAATGG
TGAATATCTTCAAATGATTTAGTATTATTTATGGCAAATACACAAAGAAAGCCCTCCCCA
GTCCTCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCGAGAATA
TCCAAGAGACAGGTTTCTCCATCAATTACTACTTGCTTCCTGTAGGAATCCTGAGAAGGG
AGAAACACAGTCTGGATTATTACAGTGCACCTTT

3) High coverage:
>NOTCH46dna:chromosomechromosome:GRCh37:6:32188398:32188711:1
CATCTTCGGAGCAGTCCCAGCCTGCAGGGGGTTGGGGAGGGGACGAGGGCTAAGGCTGGG
AGCCCTATGAGTAGGGGAGGCCAGGGGCCAACTCTCTGGGCCATGGGTGTCATGGATGTG

GCTTAAACAACTCACCTGTCCAGGTTTCTGGGCAGAGGCAGGTGTAGGTGTCCAGCCCAT
CCTGGCAAGTGCCCCCATTCTGACACTGGTGGCTGACACAGTTGTCTGGATTCACCTCAC
AGTCTGGGCCTATGAAACCTGACAGGGTCATGGATCAGCTGTGGGAGGAGGCTCCAACGG
AGACATCCTGCCCT
>PDE4DIP 1dna:chromosomechromosome:GRCh37:1:145038786:145039128:1
CTCCAAAGCTAAATCCCACAGATGGAAGCATATCTGTTATAGAGACACCGCTGGAATAGT
CTCCCTACCCTCCCACCCACTAAAAAACAGAAACGAAGTCTTTGTCTCAATACATGGTCT
CTTATCCTTACCCTTTCCTTGAATGGGGCTTCATTTTAAAGTGTGTCTGGGGGAAGGACT
GGAAGAAAACGCAGTTTCACAGGCCATCTCCAAAATCTGACCTAAAAGCCAACTGTTGCC
TGGCCTCAATAAAGCCTGAGCAGTGTGGTGCCCTGATCTCTTTCCTTCTCCATTCCTCCC
CCAAGTGGGCTCCAACTGGAGGTTGTGGAGATGAGTGCTGCTG