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    Van - Soest Procedure: Sample

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    nerissalove
    The Van - Soest procedure is a method used to analyze forage samples and determine their fiber content. It involves sequentially treating a forage sample with neutral detergent solution, acid detergent solution, and a lignin solution. This separates the sample into fractions of cell contents, neutral detergent fiber, acid detergent fiber, lignin, and insoluble ash. The procedure provides a means to determine the concentration of important forage fiber components like cellulose, hemicellulose, and lignin.

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    Van - Soest Procedure: Sample

    Uploaded by

    nerissalove
    186 views2 pages
    The Van - Soest procedure is a method used to analyze forage samples and determine their fiber content. It involves sequentially treating a forage sample with neutral detergent solution, acid detergent solution, and a lignin solution. This separates the sample into fractions of cell contents, neutral detergent fiber, acid detergent fiber, lignin, and insoluble ash. The procedure provides a means to determine the concentration of important forage fiber components like cellulose, hemicellulose, and lignin.

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    lignina

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    Van Soest

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    The Van - Soest procedure is a method used to analyze forage samples and determine their fiber content. It involves sequentially treating a forage sample with neutral detergent solution, acid detergent solution, and a lignin solution. This separates the sample into fractions of cell contents, neutral detergent fiber, acid detergent fiber, lignin, and insoluble ash. The procedure provides a means to determine the concentration of important forage fiber components like cellulose, hemicellulose, and lignin.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as doc, pdf, or txt
    186 views2 pages

    Van - Soest Procedure: Sample

    Uploaded by

    nerissalove
    The Van - Soest procedure is a method used to analyze forage samples and determine their fiber content. It involves sequentially treating a forage sample with neutral detergent solution, acid detergent solution, and a lignin solution. This separates the sample into fractions of cell contents, neutral detergent fiber, acid detergent fiber, lignin, and insoluble ash. The procedure provides a means to determine the concentration of important forage fiber components like cellulose, hemicellulose, and lignin.

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    © All Rights Reserved
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    Van - Soest procedure

    Sample
    Neutral detergent solution

    Soluble

    Insoluble (residue)

    Cell content (CC)


    Protein
    Fat
    Soluble carbohydrate
    Soluble ash

    (NDF) Cell wall


    Cellulose
    Hemicellulose
    Lignin
    Insoluble ash

    (CC % = 100 % - NDF %)

    Sample
    Acid detergent solution
    Soluble

    Insoluble (residue)

    CC

    (ADF)
    Cellulose
    Lignin
    Acid insoluble ash

    Hemicellulose

    (Hemicellulose % = NDF % - ADF %)


    ADF
    2:1 sat. KMnO4 : Lignin buffer solution
    Soluble
    Lignin

    Insoluble (residue)
    Cellulose
    Insoluble ash
    (by ashing, cellulose and insoluble ash will be separated)

    Remark:
    To adjusting to dry basis:
    Analysis % on as it is basis
    -------------------------------- X 100
    Dry matter %

    To adjusting to as it is basis:
    Analysis % on as DM basis
    -------------------------------- X Dry matter %
    100

    (This

    you need)

    References:
    Goering, H. K. and P. J. Van Soest. 1970. Forage fiber analysis (Apparatus, reagent, procedures
    and some applications). Agric. Handbook, No. 379, ARS-USDA, Washington, DC.
    Van Soest, P.J., J.B. Robertson and B.A. Lewis, 1991. Methods for dietary fiber, neutral detergent
    fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74: 3583-3597.

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