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  • Attachment: 1 Preparation The Extract of Methanol Weight 5.001 Grams of

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    Desiana Anggraeni
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    The document outlines 7 procedures to identify various chemical compounds in an extract of Curcuma zanthorrhiza: (1) preparation of a methanol extract, (2) identification of alkaloids using Culvenor-Fitzgerald, Meyer, Wagner, and Dragendorf reagents, (3) identification of flavonoids using ethanol and magnesium, (4) identification of saponins using hot water, (5) identification of steroids using ethanol and sulfuric acid, (6) identification of triterpenoids using chloroform and sulfuric acid, (7) identification of tannins using iron chloride. Pictures accompany each procedure to illustrate the steps and results.

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    Attachment Curcumic

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    The document outlines 7 procedures to identify various chemical compounds in an extract of Curcuma zanthorrhiza: (1) preparation of a methanol extract, (2) identification of alkaloids using Culvenor-Fitzgerald, Meyer, Wagner, and Dragendorf reagents, (3) identification of flavonoids using ethanol and magnesium, (4) identification of saponins using hot water, (5) identification of steroids using ethanol and sulfuric acid, (6) identification of triterpenoids using chloroform and sulfuric acid, (7) identification of tannins using iron chloride. Pictures accompany each procedure to illustrate the steps and results.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as docx, pdf, or txt
    29 views4 pages

    Attachment: 1 Preparation The Extract of Methanol Weight 5.001 Grams of

    Uploaded by

    Desiana Anggraeni
    The document outlines 7 procedures to identify various chemical compounds in an extract of Curcuma zanthorrhiza: (1) preparation of a methanol extract, (2) identification of alkaloids using Culvenor-Fitzgerald, Meyer, Wagner, and Dragendorf reagents, (3) identification of flavonoids using ethanol and magnesium, (4) identification of saponins using hot water, (5) identification of steroids using ethanol and sulfuric acid, (6) identification of triterpenoids using chloroform and sulfuric acid, (7) identification of tannins using iron chloride. Pictures accompany each procedure to illustrate the steps and results.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as docx, pdf, or txt
    of 4

    ATTACHMENT

    No.
    Procedure
    Picture
    1
    Preparation the extract of methanol curcuma zanthorrhiza
    Before
    Weight 5.001 grams of curcuma
    zanthorrhiza

    Powder of curcuma zanthorrhiza

    Methanol 60-80%

    Extraction

    Heated

    After

    15ml of methanol+5.001 grams of


    curcuma zanthorrhiza

    Sample after heated

    Identification Alkaloids by Culvenor-Fitzgerald method


    1ml of Sample+1ml of
    chloroform+1ml of ammoniac

    1ml of Sample+1ml of
    chloroform+1ml of
    ammoniac+H2SO4 2N

    Filtrate 1+ Meyer reagent


    Filtrate 2 + Wagner reagent
    Filtrate 3+ Dragen Dorf reagent

    3.

    Identification of Flavonoid

    Sample+3ml of ethanol+ 0.1 gram


    of Mg

    Sample+3ml of ethanol+ 0.1 gram


    of Mg + HCl

    4.

    Indenfitication of Saponin
    Sample+hot water

    5.

    Identification of Steroid
    Sample+ethanol+H2SO4
    concentrated

    Sample+ethanol+H2SO4
    concentrated+ acetate acid

    6.

    Identification of Triterpenoid

    Sample+chloroform

    Sample+chloroform+ H2SO4
    concentrated

    7.

    Identification of Tannin
    Sample+FeCl3

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