NAME

MATRIC

SITI KAHDIJAH BINTI AHMAD

1111314

Assignment 2 (Bionanotechnology),
Based on your understanding about nanopores from the lectures, and also from the extra reading
materials on nanopores, answer the following questions (based on the review paper):
1)What are the types of nanopores that are outlined in the paper?
Staphylococcus aureus toxin, -hemolysin,
2)What are the applications of the nanopores?
biosensing, diagnostics and separation
Measurement of ionic current blockades as ssDNA is driven througha biopore or a solidstate pore.
Measurement of ionic current blockades from individual nucleotides
Measurement of transverse tunneling currents or capacitance as ssDNA is driven through
a solid-state nanopore with embedded probes
sequentially cleaved off the end of a DNA strand and driven through a
biopore.
Measured native order of the nuclei based in polynucleotide
Measured single nucleic and polymer.
3)Discuss the selectivity of the nanopores.
Characteristic nanopore
High confined space

selectivity
single nucleic and polymer.

Perfect processivity

native order of the nuclei based in

polynucleotide

via
High throughput by one of a
variety of mean
Enforce in a narrow pore

4)What are the limitations of the nanopores research?

unravel a coiled nucleic acid because the inside diameter of the -hemolysin pore is
barely as large as the diameter of a single nucleic acid strand.
Recently high cost because price of instruments.
Need a lot for sample preparation because readout requires nucleoitides,
polymer or ligase and potential of very long tread length.

5)What are the future directions in nanopores research?

Nanopore sequence characteristic.

Identification of each successive nucleotide in a DNA strand.
Synthetic solid-state nanopore
To slow down DNA translocation from microsecond per base to millisecond.
To control stochastic motion of DNA molecules which is increased signal noise in sensor