Gastroenterology 670 INFLUENCE OF HOMOCYSTEINE ON THE PARAMETERS OF OXIDATIVE STRESS IN RAT COLON AND LIVER ESCID 670 Name Stevi¢, Jovana Country Serbia University Belgrade University School Of Medicine Co-Author(s) _ Nataga Stankovic, Dusan Vukicevic, Petar Zlatanovi¢ ABSTRACT TITLE: INFLUENCE OF HOMOCYSTEINE ON THE PARAMETERS OF OXIDATIVE STRESS IN RAT COLON AND LIVER BACKGROUND: Oxidative stress due to the production of intracellular and extracellular reactive oxygen species may be a major player in the pathogenesis of cardiovascular and other diseases. It is now well established that hyperhomocysteinemia is an independent risk factor for coronary artery disease, cere- brovascular disease, and peripheral vascular occlusive disease. Data from the literature suggest that ele- vated values of homocysteine affect the pathogenesis of some diseases of the gastrointestinal system. METHODS: In the experiment, we used male Wistar rats (n=6) aged 20-21 weeks and weighing 160 + 20g, They were raised in a barn Institute of Medical Physiology, under conditions of constant temperature, humidity and photoperiod. We measured the parameters of oxidative stress (catalase, total radical-trapping antioxidant potential - TBARS and total antioxidative status ~ TAS) in the tissue homogenates isolated colon and liver and control groups (absence of Hcy) after 30-minute incubation of intraperitonal homocysteine application (15 ymol/1). RESULTS: Acute effects of homocysteine leads to a reduction of catalase activity to 22.22% in the colon, and 17.77% in the liver, increased by TBARS (46.58% colon, 42.86% liver), and decreased by TAS (15.6 % colon, liver 24.3%). CONCLUSION: Homocysteine lesads to reduced activity of catalase, and increased in TBARS activity and decreased in TAS activity during intraperitonal application of homocysteine of liver tissue and rat colon. Results suggest that oxidative stress could be also involved in the gastrointestinal dysfunction of homocysteine high levels. However, further studies are necessary to confirm and extend findings to the human condition. 198 GASTROENTEROLOGY