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Laboratory Exercise No. 8

Urine Culture Techniques
Results:
A. Examination and Qualitative Culture of Voided Urine
1. Macroscopic appearance of urine
Specimen
Clean catch
Casual

Color

Turbidity

Light yellow

Clear

Light yellow

Slight turbidity
(flocculation)

Odor

pH
Acidic

Pungent

Acidic

2. Culture results:
BAP
Specimen

Amount of
growth

Types of
growth

EMB or McConkey
Gram stain
and
morphology

Amount of
growth

Types of
growth

Gram stain
and
morphology

Clean catch
Casual
3. Interpret any differences you observed in the amount of growth recovered from the two specimens.
4. Interpret differences in the amount of growth on blood agar and MacConkey plates for each specimen.
5. Interpret differences in the nature of growth obtained on blood agar and MacConkey plates for each
specimen.
6. Interpret any finding of no growth.
B. Quantitative Urine Culture
1. Show picture of NA plate with colonies.
2. Record the number of colonies on the streaked nutrient agar plate. Calculate and record the number of
organisms per milliliter of specimen and indicate whether this result is significant of urinary tract
infection.
3. Diagram your observations of turbidity in each tube of stored urine. What is your interpretation of the
appearance of these tubes?