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  • Characterisation of DNA

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    Lexie Rebosa
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    The document describes several biochemical tests to identify components of nucleic acids including tests for deoxyribose using diphenylamine reagent and sulfuric acid which turns blue, phosphate using nitric acid, sulfuric acid, ammonium molybdate which precipitates yellow, purines using nitric acid and potassium hydroxide which forms a dark red residue, and pyrimidines using bromine water and barium hydroxide which forms a purple precipitate.

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    Characterisation of DNA (colour reactions)

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    The document describes several biochemical tests to identify components of nucleic acids including tests for deoxyribose using diphenylamine reagent and sulfuric acid which turns blue, phosphate using nitric acid, sulfuric acid, ammonium molybdate which precipitates yellow, purines using nitric acid and potassium hydroxide which forms a dark red residue, and pyrimidines using bromine water and barium hydroxide which forms a purple precipitate.

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    © All Rights Reserved
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    9 views1 page

    Characterisation of DNA

    Uploaded by

    Lexie Rebosa
    The document describes several biochemical tests to identify components of nucleic acids including tests for deoxyribose using diphenylamine reagent and sulfuric acid which turns blue, phosphate using nitric acid, sulfuric acid, ammonium molybdate which precipitates yellow, purines using nitric acid and potassium hydroxide which forms a dark red residue, and pyrimidines using bromine water and barium hydroxide which forms a purple precipitate.

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    of 1

    Test

    Reagents

    Positive result

    Notes

    Test for deoxyribose


    (Dische)

    Diphenylamine reagent, conc.


    H2SO4 standard deoxyribose
    solution

    Dark blue solution

    Conc. H2SO4 dehydrates


    deoxyribose
    Diphenylamine

    complexation; creates the


    blue-coloured solution

    Test for phosphate

    Concentrated HNO3,
    concentrated H2SO4,
    standard phosphate solution,
    25% ammonium molybdate

    Clear liquid solution with


    yellow ppt

    Aims to precipitate phosphate


    Ammonium
    phosphomolybdate
    - yellow

    Test for purines (Murexide)

    Concentrated HNO3, 10%


    KOH, standard
    guanine/adenine solution

    Dark red residue with yellow


    solution along the periphery

    Bromine water, Ba(OH)2


    Standard cytosine or uracil
    solution

    Turbid solution with purple


    precipitate

    HNO3 oxidises purine


    Forms dialuric acid
    and alloxan
    Evaporating to dryness
    Condensation
    reaction
    Alloxan alloxanthin
    KOH used to neutralise
    Forms murexide (redpurple in colour)
    Potassium salt
    Turns yellow when
    diluted, red when
    evaporated
    Excess bromine water
    bromination of pyrimidine
    Oxidises in dialuric
    acid
    Ba(OH)2 neutralises acid
    Forms a barium salt
    (purple ppt)

    Test for pyrimidines


    (Wheeler-Johnson)

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