DNA polymerase exonuclease activity is

required to remove RNA primers. Which exo

performs this functions?
A.
B.
C.

5 to 3
3 to 5
Either one

A.
B.
C.
D.

Which of the following enzymes

break the phosphodiester
backbone
and
then
remake
it?
Helicase
Ligase
Topoisomerase
DNA polymerase

Which of the following sequences would be

found in a telomerase in a cell with the repeat
sequence TTAGGG?
A.
B.
C.
D.

AATCCC
CCCTAA
AAUCCC
CCCUAA

Which of the following could cause

a transversion?
A.
B.
C.
D.

Ethidium bromide
5-bromouracil
Deamination of A or C
Replication error

Which of the following can be

repaired by mismatch repair
system?
A.
B.
C.
D.

Thymine dimer
A-C base pair
Methylated G
Single base insertion

Which of the following enzymes

has a 3 sticky end?
A.
B.
C.
D.

CT^GCAG
G^GATCC
CC^GG
AGATC^T

Suppose you insert a gene between the BamHI

and SalI sites in pBR322, what will be the drug
resistance of the resulting plasmid?
A.
B.
C.
D.

Tetr, Ampr
Tets, Ampr
Tetr, Amps
Tets, Amps

Could Telomerase be used to make a cDNA

library?

A.
B.

yes
no

When screening for HIV infection by

western blot, what is the source of proteins
loaded on the gel?
A.
B.

The patients blood

Purified HIV proteins

Which primer set below will amplify

segment A?
5
3

A.
B.
C.
D.

primer 1
primer 2

Primer 1 and primer 3

Primer 2 and primer 4
Primer 1 and primer 4
Primer 2 and primer 3

primer 3 3
5

primer 4

What would happen if you used two primers

for DNA sequencing (similar to PCR)?
A.
B.

You would get one sequence of the region

between the primers
You would see two bases at each position