Brain Heart Infusion - Blood Agar (BHI-BA)

Purpose: BHI-BA is an enriched, non-selective media for the

cultivation and isolation of a wide variety of bacteria, including
yeast and molds. Horse blood stimulates growth.
Formula: (cat no. DF0418177, ordered from Fisher Scientific)
g/L
Calf Brains, infusion

7.7 g

Beef Heart, infusion

9.8 g

Proteose peptone

10.0 g

Dextrose

2.0 g

Sodium Chloride

5.0 g

Sodium Phosphate

2.5 g

Agar

15.0 g

pH 7.4 +/- 0.2 (do not need to pH, comes from pre-made media
bottle)
Bring to a boil to dissolve completely
Autoclave at 121 C for 15 min
Cool to 50 C in water bath
Aseptically add 10% defibrinated horse blood (Lampire, cat no
50412331; ordered from Fisher Scientific)
o

Special Notes:
1. Add 26 gram of pre-made BHI Agar powder to 450 mls DI
water
2. Let defibrinated horse blood come to room temperature
before adding to media
3. 500 mls of media will pour 20 plates (1 sleeve)