Bacterial

Growth
Rates
Unit 3 Assignment 1

Mari Soto (16658809)

1. Feces contains fecal bacteria and EMB agar grows coliforms (bacteria found in the
intestines of warm-blooded animals) really well! EMB you know something will grow if
its fecal matter. And MacConkey Agar(MCA) or Xylose Lysme Desoxycholate (XLD) If
the culture is not Shigella you will get no growth on the media listed above, whereas you
should get growth in a. If it is E. coli, in particular, the growth you see in a. should be
metallic and green.
2. Take the mixed culture and grow it in a broth so the numbers are higher and are happy.
You will use selective media to separate them with the t-streak method. To then isolate
the Pseudomonas, you use Pseudomonas Isolation Agar (PLA). For the Staph, grow on
Mannitol salts. S. aureus will change the media yellow, the others will not. To then isolate
the individual strains, select individual CFU, one red and one yellow. Once youve
isolated the three individual bacteria, you can inoculate three separate media broths, one
each with one of the bacteria.
3.
Day
Actual
Rounded/Predicte

1
43
45

2
90
90

3
172
180

4
?
360

5
?
720

6
?
144

7
?
288

8
?
576

9
10
?
?
11520 23040

d
0
0
0
By day 6 you will have a full bloom and you can do plate counts after doing serial
dilutions instead of doing microscope direct counts.
4.

9
0
2

9:30
2
2:30

10
4
3

10:30
8
3:30

11
16
4

11:30
32
4:30

12
64
5

12:30
128
5:30

1
256
6

1:30
512
6:30
7:30

1024

2048

4096

8192

16384

32768

65536

13107

26214

52428

8
10485
76

12pm=64cells, 7pm= 20 generations, if you leave it indefinitely it will stop growing and
it will
hit the death phase.

5.
Time
Min
Gen
Bac

230
330
11
2048

3
330
360 390
12
13
4096 8192

4
420
14
16384

430
450
15
32768

5
480
16
65536

530
510
17
131072

6
540
18
262144

630
570
19
524288

As with any culture, if you leave it indefinitely, the bacteria will go into death phase and
this will end up with no viable bacteria. A decrease in nutients combined wih an increase
in wastes will limit growth.
5. Last years dilution count was the 1:100 and it yielded roughly 49cfus which would
suggest there were approximately 4,900 bacteria per mL of sample last year. This year
you would select 1:1000 dilution and the count was roughly 45, which would indicate
approximately 45,000 bacteria per mL of sample this year. The results suggest that there
is a marked increase in bacteria and that research must be done to determine what could
be causing the boom in bacteria.

7
600
20
1048576