2) The negtative question form makes a request more polite

Won't you come tomorrow at 8 O'Clock?

3) emphasizes the speakers request and makes it more polite.

Go ahead please, and eat as much as you like

4) In very informal speech, kudasai may drop(this form of request is often used by
female speakers)

go on say it (please)

dont come back yet (please)

5) , the imperative form of kureru, can also be used in place of kudasai in informal
male speech.[Vte/Vneg nai de] kure is the least polite request form.

can you come together (for me?)

(=can you let me go with you?)