DNA

Fingerprinting
Group 6 IV-A BSBT
Arrojo, Jesus Jr. P.
Barrios, Jasmin T.
Cruspero, Carmi Marie C.
Fruelda, Gladyz F.

Definition:
- is method of isolating
and making images of
sequences ofDNA.

Outline:
1. Getting a DNA
Sample
2. Restriction of
DNA
3. Polymerase Chain
Reaction

4. Gel
Electrophoresis
5. Denaturation

6. DNA Hybridization
7. Autoradiography

Getting a DNA Sample

1. Isolate from blood cells, semen, hair root or
cheek cell.

Getting a DNA Sample

2. Sodium Dodecyl Sulfate (SDS) will be
added to the sample.
3. Protease will be added to damage the
proteins.
Pineapple juice Bromelain
Papaya - Papain
4. Ethyl alcohol will be added to separate the
DNA from other organic substances.

Restriction of DNA
Restriction Enzymes

Source:
Arthobacter luteus

Haemophilus aegyptius

Bacillus amyloliquefaciens

Haemophillus influenzae Rd

Escherichia coli strain R

Polymerase Chain Reaction

Denaturation (90-95C)
5
ATGAAATTTATCGCTACC
3
3
5
TACTTTAAATAGCGATGG
ATGAAATTTATCGCTACC
5
3

Polymerase Chain Reaction

Annealing
Primers

(50C - 70C)

5
ATGAAATTTATCGCTACC
3
TAC
ACC

Polymerase Chain Reaction

Extension
(70C and
75C)
Taq polymerase
Thermopillus
aquaticus

5
ATGAAATTTATCGCTACC
3
TAC

Polymerase Chain Reaction

5
ATGAAATTTATCGCTACC
3
3
TACTTTAAATAGCGATGG
5
5 ATGAAATTTATCGC
TACC 3

Gel Electrophoresis
Agarose Gel
Polyacrylamide Gel

Denaturation

DNA Hybridization

Buffer
Solution

Autoradiography

Applications:
Forensics

Applications:
Diagnosis of Inherited
Disease

Applications:
Parentage Testing

Applications:
Tracing the Origin of a
Particular Plant Sample