What kind of enzyme

synthesizes
the new DNA
RNA polymerase
strand?
DNA Polymerase
1)

2)
3)

Primase

4)

Helicase

5)

Topoisomerase

In what direction is the newly

synthesized DNA produced?
1.

5'-3'

2.

3'-5'

3.
4.

5.

In the direction of the major groove

In the direction of the minor groove
Both 5'-3' and 3'-5' depending on
which strand is being replicated

Nucleotides are always added to the

growing DNA strand at the 3 end, at
which the
DNA
has a free
______ on the 3
1.
Phosphate
group
carbon of its terminal deoxyribose.
2.
Hydroxyl group
3.

Nitrogen base

4.

Methyl group

5.

Carboxyl group

The E. coli chromosome has 4.7x106 bp; a

bi-directional replication fork progresses
at about 1000 nucleotides/sec. Therefore,
the minimum time required to complete
replication is
1)

12 min.

2)

24 min.

3)

39 min

4)

78 min

5)

120 min

What is the sequence (1 to 6) in which

these proteins function during DNA
replication

____ RNA primase

__ DNA ligase
____ DNA polymerase
____ Topoisomerase
____ DNA helicase
___ tethering proteins

Why is an RNA primer necessary for

DNA replication?
A.

B.

C.

D.

E.

The RNA primer is necessary for the activity of

DNA ligase.
The RNA primer creates the 5 and 3 ends of the
strand.
DNA polymerase can only add nucleotides to RNA
molecules.
DNA polymerase can only add nucleotides to an
existing strand
DNA polymerase is low processice enzyme