Biotechnology Worksheet # 2

Name ____________________________

Base your answers to questions 1 through 4 on the information and diagram below
and on your knowledge of biology.
The four wells represented in the diagram were each injected with
fragments that were prepared from DNA samples using identical techniques.

_______1. This laboratory procedure is known as (1) cloning (2) gel electrophoresis
(3) chromatography (4) use of a dichotomous key
2. The arrow represents the direction of the movement of the DNA fragments. What is
responsible for the movement of the DNA in this process?

3.. The four samples of DNA were taken from four different individuals. Explain how this
is evident from the results shown in the diagram.

4.. Identify the substance that was used to treat the DNA to produce the fragments that
were put into the wells.

5. Which DNA band of the bands identified as 1 through 5 is the smallest. Justify your answer.

Biotechnology Worksheet # 2

Name ____________________________

Use the diagram of a technique in molecular biology and your knowledge of biology to answer
questions 6 to 8 that follow.

________6.

The technique being used to assess this information is called

(1.) human genome determination (2.) karyotyping
(3.) transformation (4.) DNA blotting (5.) electrophoresis

________7.

Based on the indicated position of the wells, the longest DNA fragment in
the crime scene sample is at position (1.) can not be determined by
the information given (2.) A (3.) B (4.) C (5.) D

________8. Based on the results of this forensic investigation, it is likely that

the guilty individuals is individual (1.) 1 (2.) 2 (3.) 3 (4.) 4
________9.

The role of agar gel in biotechnology is to (1.) combine segments

of DNA into bacteria (2.) make enough copies of a DNA sequence for analysis
(3.) produce a plastic that does not conduct electricity (4.) cut a section of a
gene from a genetic sequence for analysis (5.) serve as a matrix for separating
DNA fragments by size

10-11. Explain how the processes of amnioscentesis and karyotyping could be used to detect
a fetus with Down syndrome.