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    CH4303-Bioseparation Tutorial 3

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    WeimingTan
    Alpha (α) is a dimensionless number used in chromatography to describe how selectively a solute distributes between the stationary and mobile phases. It is defined as the ratio of the time a solute spends in the mobile phase to the time it spends in the stationary phase during the chromatographic process. Alpha values typically range from 0 to 1. An α value close to 0 indicates that the solute spends most of its time in the stationary phase and elutes later. An α value close to 1 means the solute spends very little time interacting with the stationary phase and elutes earlier. Alpha is useful for comparing how strongly different solutes interact with the stationary phase during chromatography.

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    CH4303-Bioseparation Tutorial 3

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    WeimingTan
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    Alpha (α) is a dimensionless number used in chromatography to describe how selectively a solute distributes between the stationary and mobile phases. It is defined as the ratio of the time a solute spends in the mobile phase to the time it spends in the stationary phase during the chromatographic process. Alpha values typically range from 0 to 1. An α value close to 0 indicates that the solute spends most of its time in the stationary phase and elutes later. An α value close to 1 means the solute spends very little time interacting with the stationary phase and elutes earlier. Alpha is useful for comparing how strongly different solutes interact with the stationary phase during chromatography.

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    Tutorial 3

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    Tutorial 3

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    Alpha (α) is a dimensionless number used in chromatography to describe how selectively a solute distributes between the stationary and mobile phases. It is defined as the ratio of the time a solute spends in the mobile phase to the time it spends in the stationary phase during the chromatographic process. Alpha values typically range from 0 to 1. An α value close to 0 indicates that the solute spends most of its time in the stationary phase and elutes later. An α value close to 1 means the solute spends very little time interacting with the stationary phase and elutes earlier. Alpha is useful for comparing how strongly different solutes interact with the stationary phase during chromatography.

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    18 views2 pages

    CH4303-Bioseparation Tutorial 3

    Uploaded by

    WeimingTan
    Alpha (α) is a dimensionless number used in chromatography to describe how selectively a solute distributes between the stationary and mobile phases. It is defined as the ratio of the time a solute spends in the mobile phase to the time it spends in the stationary phase during the chromatographic process. Alpha values typically range from 0 to 1. An α value close to 0 indicates that the solute spends most of its time in the stationary phase and elutes later. An α value close to 1 means the solute spends very little time interacting with the stationary phase and elutes earlier. Alpha is useful for comparing how strongly different solutes interact with the stationary phase during chromatography.

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    CH4303-Bioseparation Tutorial 3

    Question: What is the value of alpha?

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