Standard Curve

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Concentration

Blue channel
100
108.077
50
140.272
25
140.926
12.5
162.637
10
205.112
1
211.418
0.1
214.151
0.01
223.007

Concentration

100

Lo
200
150
Color Intensity

f(
R

100
50
0
0

20

Con

Hi
250
200

f(x) =
R =

150
Color Intensity

100
50
0
0

Co

Low Sensetivity
200
150
Color Intensity

f(x) = - 0.5550664348x + 163.9967391304


R = 0.9128996632
Blue channel

100

Linear (Blue channel)

50
0
0

20

40

60

80

100 120

Concentration mg/ml

High Sensetivity
250
200

f(x) = - 3.1393256054x + 218.3089362798


R = 0.8804710243
Blue channel

150
Color Intensity

Linear (Blue channel)

100
50
0
0

10

15

20

25

Concentration mg/ml

30

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