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    The document describes an experiment to measure the concentration of dissolved oxygen (DO) in water samples over time exposed to light and dark conditions. It is hypothesized that the DO level will increase in samples exposed to light due to photosynthesis, and will decrease in dark samples due to cellular respiration. The materials and procedure for collecting initial and final DO measurements are provided. While the light sample showed an increased DO, contradicting the hypothesis, the dark sample unexpectedly increased as well, possibly due to experimental error. Questions are included about calculating respiration and productivity rates, factors influencing productivity, and developing additional research questions.

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    The document describes an experiment to measure the concentration of dissolved oxygen (DO) in water samples over time exposed to light and dark conditions. It is hypothesized that the DO level will increase in samples exposed to light due to photosynthesis, and will decrease in dark samples due to cellular respiration. The materials and procedure for collecting initial and final DO measurements are provided. While the light sample showed an increased DO, contradicting the hypothesis, the dark sample unexpectedly increased as well, possibly due to experimental error. Questions are included about calculating respiration and productivity rates, factors influencing productivity, and developing additional research questions.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    57 views5 pages

    Primary Productivity Purpose

    Uploaded by

    api-283804229
    The document describes an experiment to measure the concentration of dissolved oxygen (DO) in water samples over time exposed to light and dark conditions. It is hypothesized that the DO level will increase in samples exposed to light due to photosynthesis, and will decrease in dark samples due to cellular respiration. The materials and procedure for collecting initial and final DO measurements are provided. While the light sample showed an increased DO, contradicting the hypothesis, the dark sample unexpectedly increased as well, possibly due to experimental error. Questions are included about calculating respiration and productivity rates, factors influencing productivity, and developing additional research questions.

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    © All Rights Reserved
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    of 5

    PrimaryProductivity

    Purpose
    ToinvestigatetheconcentrationofDOinthewatersampleovertimeandtolearnhowto
    useaDissolvedOxygenProbetomeasuretheDOlevelofawatersample.

    Hypothesis

    Ifthewatersamplecontainingproducersareexposedtolight,thentheconcentrationof

    DOwillincrease.

    Ifthewatersamplecontaininglivingorganismsiskeptinthedark,thentheconcentration

    ofDOwilldecreaseduetocellularrespiration.

    Materials

    Dissolveoxygenprobe
    Beaker
    100mLofDistilledwater
    Watersample
    Lamp
    Foil
    2samplebottles

    Procedure:
    1.)Preparethedissolvedoxygenprobeforusefollowinginstructionsfromyourteacher.Place
    theprobeinabeakerfilledwithabout100mLofdistilledwater.
    2.)Connectthedissolvedoxygenprobetotheinterfaceandopenthedatacollectionprogram.
    Allowtheprobetostayinthewaterfor5minutesastheprobewarmsup.
    3.)CollectDOdata

    a.Placethetipoftheprobeintothewatersamplebeingtested.Submergetheprobetiptoa
    depthof46cm.
    b.Startdatacollection.Gentlystirtheprobeinthewatersample.Note:Itisimportantto
    keepstirringuntilyouhavefinishedcollectingdata
    c.Continuestirringanddatacollectionuntilthereadingshavebeenrelativelystable(stable
    tothenearest0.2mg/L)forabout30seconds,thenstopdatacollection.
    d.Selectthestableregionofyourgraph,thendisplayStatisticsforthatregion.Noteand
    recordthemeanvalueforthatregionastheDOofthewatersample.
    4.)Wraponebottlewiththefoil.
    5.)Placedthemunderthelightfor23hours.ThencollectDOdataagain.

    Results

    DOofthewatersample(unitmg/L)

    initial

    final

    light

    6.60

    7.94

    dark

    7.14

    7.48

    Time:23hours

    Discussion
    Ourhypothesiswasthe
    finalDOofthelightbottlewillincreaseandthefinal

    DOofdark

    bottlewilldecrease.Theresultoflightbottleis6.60mg/LforinitialDOand7.94mg/Lforfinal
    DO.Theresultofdarkbottleis7.14
    mg/LforinitialDOand7.48mg/LforfinalDO.Theresult

    isnotfollowthehypothesis.Theerrormistakeofthedarkbottlemightbewhileweweretesting
    theDOofthelightbottle,wehadalreadytakenoffthefoilonthedarkbottle.Therefore,the
    finalDOofdarkbottlemightincorrect.Wecanimproveourexperimenttobebetterbyrepeating
    theexperimentatless3times,theniftheresultofthedarkDOstillincreases,wemightconclude

    thattherearesomelivingthingsthatcanproduceoxygenduringthenight.Andthismighthelp
    ustodoourownexperimentunderthegiventopic.

    Questions
    1.WhatwastheDOofthewatersampleyoutestedinthePreliminaryActivity?

    Answer
    TheinitialDOofwatersampleinthelightbottlewas6.60mg/Land7.14
    mg/Linthe

    darkbottle.

    2.Onlyrespirationcanoccurinadarkbottle.RespirationrateisthedecreaseinDOover
    time.Supposeadarkbottlesampleofthewateryoutestedabovewastested24hourslater
    andfoundtohaveaDOlevel1.2mg/LlowerthantheinitialDOvalueyouobtained.
    Calculatetherespirationrate(inmg/L/hr.)usingtheformulation

    Answer
    Respirationrateis0.05mg/L.

    3.Supposealightbottlesampleofthewateryoutestedabovewasplacednexttoalight
    source.Supposeitwastested24hourslaterandfoundtohaveaDOlevel0.5mg/Lhigher
    thentheinitialDOvalueyouobtained.Calculategrossproductivity(inmg/L/hr)usingthe
    formula

    Answer

    Grossproductivityis0.071mg/L.

    4.Calculatenetproductivity(inmg/L/hr.)usingtheformula

    Answer
    Netproductivityis0.021mg/L.

    5.Listsomelocalsourcesofpond,lake,orseawaterthatmightbeusefulinthis
    experiment.
    Answer
    Pool
    Waterfalls
    Brackishwater

    6.Listsomefactorsthatmightinfluenceprimaryproductivityinanaquaticenvironment
    .
    Answer
    Human
    Waste
    Factory
    Temperature
    Weather

    7.
    Listatleastoneresearchablequestionforthisexperiment.

    Answer
    WhydidDOinthedarkbottleincrease?
    Isitpossiblethatinthewatersamplehaslivingthingsthatcanproduceoxygenindark?

    Editby:
    (Section104)
    MissNapachatChanakit
    MissThanyamasChanchaiwat
    MissKamonwanEmong
    MissNutnichaPhokai

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