2009250026

Contents

Introduction
LCM, ICAT, 2D-LC-MS, Tandem Mass
Materials & Procedures
Result
Conclusion

3
Carcinoma( )

Micrographofsmall cell carinomaof the lung, alsosmall cell lung cancer.

FNA specimen.Field stain. The image shows the key features of small cell lung
cancer (SCLC): Nuclear moulding.Salt and pepper chromatin. Scant
cytoplasm.Small cellis so named as the tumour cells are small relative to
othercarcinomas, e.g.adenocarcinoma; small cell has large cells relative to
most lymphomas.Related imagesSquamous cell carcinoma.

4
Hepatocellular Carcinoma( )
2002 21.7%

25 ~ 100

LCM Carcinoma, Non-carcinoma Cell

Lysing Toludine Blue ,
Trypsin
ICAT Labeling Fraction
2D-LC, 1D-LC
MS/MS

6
Laser Capture Microdissection(LCM)

In Vivo

7
(Cleavable)Isotope-Coded Affinity Tag(ICAT)

8
Tandem Mass(MS/MS)

9
Tandem Mass(MS/MS)

CID : collision induce dissociation 1 collision cell

fragmentation .

10
Result

11
Result

12
Result

13
Result

14

Thank you!