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  • Thermus Aquaticus, A Bacterium That Grows in Hot Pools As A Result It Is Not

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    Polymerase chain reaction (PCR) is a technique used to amplify specific DNA sequences. It involves using primers and DNA polymerase to exponentially copy the target sequence. A thermo-stable DNA polymerase from Thermus aquaticus allows amplification without adding new polymerase each cycle. After many cycles, the amplified DNA can be detected using gel electrophoresis. Real-time PCR enables both detection and quantification of DNA by measuring fluorescence from DNA-binding dyes at each amplification cycle, allowing for quantification of viral loads. PCR can identify Herpes Zoster virus and determine the amount of virus present.

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    PCR

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    Pcr

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    Polymerase chain reaction (PCR) is a technique used to amplify specific DNA sequences. It involves using primers and DNA polymerase to exponentially copy the target sequence. A thermo-stable DNA polymerase from Thermus aquaticus allows amplification without adding new polymerase each cycle. After many cycles, the amplified DNA can be detected using gel electrophoresis. Real-time PCR enables both detection and quantification of DNA by measuring fluorescence from DNA-binding dyes at each amplification cycle, allowing for quantification of viral loads. PCR can identify Herpes Zoster virus and determine the amount of virus present.

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    19 views1 page

    Thermus Aquaticus, A Bacterium That Grows in Hot Pools As A Result It Is Not

    Uploaded by

    smithaanne20016923
    Polymerase chain reaction (PCR) is a technique used to amplify specific DNA sequences. It involves using primers and DNA polymerase to exponentially copy the target sequence. A thermo-stable DNA polymerase from Thermus aquaticus allows amplification without adding new polymerase each cycle. After many cycles, the amplified DNA can be detected using gel electrophoresis. Real-time PCR enables both detection and quantification of DNA by measuring fluorescence from DNA-binding dyes at each amplification cycle, allowing for quantification of viral loads. PCR can identify Herpes Zoster virus and determine the amount of virus present.

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    of 1

    Smitha Abraham

    Case 3.1
    PCR and HZ

    Polymerase Chain Reaction technique used to amplify sections of DNA ,


    generating millions of copies of a particular DNA sequence.
    Used for the detection and diagnosis of diseases, among other things
    by identifying the genes which are different, and therefore unique, one can
    use this information to identify an organism
    PCR entails the use of a pair of primers, each about 20 nucleotides in length,
    that are complementary to a defined sequence on strands of the DNA. These
    primers are extended by a DNA polymerase so that a copy is made of the
    designated sequence
    This leads to exponential amplification. Since it is necessary to raise the
    temperature to separate the two strands of the double strand DNA in each
    round of the amplification process, a major step forward was the discovery of
    a thermo-stable DNA polymerase (Taq polymerase) that was isolated from
    Thermus aquaticus, a bacterium that grows in hot pools; as a result it is not
    necessary to add new polymerase in every round of amplification. After
    several (often about 40) rounds of amplification, the PCR product is analyzed
    on an agarose gel and is abundant enough to be detected with an ethidium
    bromide stain
    The high sensitivity of PCR permits virus detection soon after infection and
    even before the onset of disease. Such early detection may give physicians a
    significant lead in treatment. The amount of virus ("viral load") in a patient
    can also be quantified by PCR-based DNA quantitation techniques
    real-time polymerase chain reaction, also called quantitative real time
    polymerase chain reaction (Q-PCR/qPCR/qrt-PCR) or kinetic polymerase chain
    reaction (KPCR), is a laboratory technique based on the PCR
    Real Time-PCR enables both detection and quantification. A DNA-binding dye
    binds to all double-stranded (ds)DNA in PCR, causing fluorescence of the dye.
    An increase in DNA product during PCR therefore leads to an increase in
    fluorescence intensity and is measured at each cycle, thus allowing DNA
    concentrations to be quantified
    Thus, PCR can be used to identify Herpes Zoster as well as determine the
    viral load

    REFERENCES:
    http://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechPCR.shtml (pcr & real
    time qrt pcr)

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