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    Simmons Citrate Agar: Minimum Q.C. Organisms: E. Coli NCIMB 50034

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    kierkegard
    Simmons Citrate Agar is a culture medium developed in 1926 by Simmons to differentiate enteric bacteria and isolate fungi. Certain bacteria in the Enterobacteriaceae family can use citrate as the sole carbon source and ammonium salts as the sole nitrogen source, increasing the pH and changing the medium color from green to blue. Inoculated bacteria are streaked on the surface and stabbed into the butt of the agar slants. Interpretation involves observing growth and any color change from green to blue, indicating citrate and ammonium salt utilization. Salmonella species, Serratia, Klebsiella, Proteus, and Citrobacter freundii typically grow and change the color to blue.

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    Simmons Citrate Agar: Minimum Q.C. Organisms: E. Coli NCIMB 50034

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    kierkegard
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    Simmons Citrate Agar is a culture medium developed in 1926 by Simmons to differentiate enteric bacteria and isolate fungi. Certain bacteria in the Enterobacteriaceae family can use citrate as the sole carbon source and ammonium salts as the sole nitrogen source, increasing the pH and changing the medium color from green to blue. Inoculated bacteria are streaked on the surface and stabbed into the butt of the agar slants. Interpretation involves observing growth and any color change from green to blue, indicating citrate and ammonium salt utilization. Salmonella species, Serratia, Klebsiella, Proteus, and Citrobacter freundii typically grow and change the color to blue.

    Original Description:

    biologia molecular

    Original Title

    LAB069 Specification

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    Simmons Citrate Agar is a culture medium developed in 1926 by Simmons to differentiate enteric bacteria and isolate fungi. Certain bacteria in the Enterobacteriaceae family can use citrate as the sole carbon source and ammonium salts as the sole nitrogen source, increasing the pH and changing the medium color from green to blue. Inoculated bacteria are streaked on the surface and stabbed into the butt of the agar slants. Interpretation involves observing growth and any color change from green to blue, indicating citrate and ammonium salt utilization. Salmonella species, Serratia, Klebsiella, Proteus, and Citrobacter freundii typically grow and change the color to blue.

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    4 views1 page

    Simmons Citrate Agar: Minimum Q.C. Organisms: E. Coli NCIMB 50034

    Uploaded by

    kierkegard
    Simmons Citrate Agar is a culture medium developed in 1926 by Simmons to differentiate enteric bacteria and isolate fungi. Certain bacteria in the Enterobacteriaceae family can use citrate as the sole carbon source and ammonium salts as the sole nitrogen source, increasing the pH and changing the medium color from green to blue. Inoculated bacteria are streaked on the surface and stabbed into the butt of the agar slants. Interpretation involves observing growth and any color change from green to blue, indicating citrate and ammonium salt utilization. Salmonella species, Serratia, Klebsiella, Proteus, and Citrobacter freundii typically grow and change the color to blue.

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    Simmons Citrate Agar

    LAB 69
    Description
    A medium devised by Simmons in 1926 to help in the differentiation of enteric bacteria and in the isolation of fungi. Certain
    Enterobacteriacae have the ability to utilize citrate as the sole source of carbon and utilize inorganic ammonium salts as the sole source of
    nitrogen resulting in an increase in alkalinity. Bromothymol Blue is used as a pH indicator.
    Formula
    Magnesium sulphate
    Ammonium dihydrogen phosphate
    Dipotassium phosphate
    Sodium citrate
    Sodium chloride
    Bromothymol blue
    Agar No. 2

    g/litre
    0.2
    1.0
    1.0
    2.0
    5.0
    0.08
    15.0

    Method for reconstitution


    Weigh 24 grams of powder, disperse in 1 litre of deionised water. Allow to soak for 10 minutes, swirl to mix then heat to dissolve the agar
    and solids. Dispense into tubes or bottles then sterilise by autoclaving at 121C for 15 minutes. Allow to set as slopes.
    Appearance: Green, opalescent.
    pH: 6.9 0.2
    Minimum Q.C. organisms:

    E. coli NCIMB 50034


    C. freundii NCTC 9750

    Storage of Prepared Medium: Capped containers up to 3 months at 15-20C in the dark.


    Inoculation: Streak on surface and stab into the butt.
    Incubation: 37C aerobically for 24-48 hours, with loose caps to allow gaseous exchange.
    Interpretation: Utilisation of citrate and ammonium salts results in growth and a change in colour of the medium from green to blue.
    Growth Characteristics
    organism
    (most) Salmonella spp
    S. typhi
    S. cholerasuis
    S. gallinarum
    S. paratyphi A
    Serratia spp.
    Shigella spp.
    Yersinia spp.
    Klebsiella spp.
    Proteus mirabilis
    P. vulgaris
    Providencia rettgeri
    P. providenciae
    E. coli
    C. freundii

    growth
    yes
    no
    no
    no
    no
    yes
    no
    no
    yes
    yes
    yes
    no
    no
    no
    yes

    colour of medium
    blue
    green
    green
    green
    green
    blue
    green
    green
    blue
    blue
    blue
    green
    green
    green
    blue

    References
    Simmons, J.S. (1926). A Culture medium for differentiating organisms of typhoid - colon aerogenes groups and for isolation of certain
    fungi. J.Inf. Dis. 39: 209-215.
    Koser, S.A. (1923). Utilisation of the salts of organic Acids by the Colon-aerogenes group. J. Bact. 8: 493-520.
    MacFaddin, J.F. (1983). Biochemical Tests for Identification of Medical Bacteria. Williams and Wilkins.

    MMv1 09/06

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