OD

reading
from
True OD
spectroph of sample % lysis
otometer.

Tube No

0.159

0.019

0.272

0.293

0.153

2.192

0.455

0.315

4.519

0.14

6.98

6.84

97.994

0.272
2.192
4.519
500 0
97.994

450

400

350

300

Chart Title
150
300
450

f(x) = - 3.217405466x^2 + 86.0526870683x + 126.8317056434

300

250

200

150

100

50

0.5

1.5

2.5

Sheep

Gelatin

erythrocy
Veronal
tes (l)

Diluted
guinea

Distilled
water

Graph T

pig serum (l)

Tube No
(l) made
in step 3

Buffer

(GVB) (l)
1
2
3
4
5

Chart Title

150
150
150
150
150

825
675
525
975
0

150
300
450
0
0

0
0
0
0
975

Percentage ofErythrocytes Lys

0.272
2.192
4.519
100

50

0.272
2.192
4.519

2.5

150
300
450

3.5

4.5

Graph To Show The Percentage Of Erythrocyte Cells Lyse

100
90
80
70
f(x) = 0.0402707 exp( 1.8444846557 x )

60

ge ofErythrocytes Lysed (%)

50
40
30
20
10
0
0

Axis Title

53630757.572

linear = 5399

5
4.5
4

y = -20.888x2 + 192.89x

50

3.5
3

-42575.5
polynomial = -42575.5

437.352

2.5
2
1.5
1

f(x) = 2

3.5
3
2.5
2
1.5
1
0.5
0
100

150

cyte Cells Lysed

4846557 x )

Chart Title
5

.5
4

.5
3

.5
2

.5
1

f(x) = 2.35590643274854E-005x^2 - 0.0004142105x

.5
3

.5
2

.5
1

.5
0
100

150

200

250

300

350

400

450

400

450

500