Bahan-Bahan Penelitian:

1. Semen Kerbau
2. Semen sapi Simental, Brahman, Limousin, Bali
3. Albumin Telur
4. Alkohol 70%
5. Pewarna eosin 10%
6. Kafein
7. Pengencer Andromed
8. Antibiotik penisilin streptomisin
9. Spritus
10. Kuning itik telur
11. Air kelapa hijau muda
12. NaCL 0,3%
13. Lubricant
14. Aquadest

Alat-Alat Penelitian
1. Vagina buatan
2. Tabung reaksi
3. Gelas ukur
4. Pipet volume
5. Pipet tetes
6. Sentrifuge
7. Termometer
8. Objek glass
9. Deck glass
10. Mikroskop

11. Spoit
12. Kontainer lengkap
13. Water bath
14. Filling shilling
15. Tabung erlenmeyer
16. Striver
17. Straw printing
18. Saringan
19. Kertas lakmus
20. Lemari es
21. Haemocytometer Improved Neubaueri