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PEWARISAN SIFAT-BioUm
PEWARISAN SIFAT-BioUm
PEWARISAN SIFAT-BioUm
: Double Heliks
1 basa nitrogen
1 gula deoksi ribosa
BASA NITROGEN:
PURIN
ADENIN
GUANIN
nukleosida
nukleotida
PIRIMIDIN
TIMIN
CITOSIN
polinukleotida
Basa Nitrogen
Gula Deoksi Ribosa
Ikatan Phosphodiester
Phosphat
The central dogma. Image from Purves et al., Life: The Science of Biology, 4th Edition, by Sinauer Associates
(http://www.sinauer.com/) and WH Freeman (http://www.whfreeman.com/), used with permission.
1. Replikasi DNA
Semiconservative replication would produce two DNA molecules, each of which
was composed of one-half of the parental DNA along with an entirely new
complementary strand. In other words the new DNA would consist of one new and
one old strand of DNA. The existing strands would serve as complementary
templates for the new strand.
The semiconservative model of DNA structure. Image from Purves et al., Life: The Science of Biology, 4th
Edition, by Sinauer Associates (http://www.sinauer.com/) and WH Freeman (http://www.whfreeman.com/),
used with permission.
The role of enzymes in opening the DNA molecule for replication. The above image is from
http://www.biosci.uga.edu/almanac/bio_103/notes/may_22.html.
Since the DNA strands are antiparallel, and replication proceeds in thje 5'
to 3' direction on EACH strand, one strand will form a continuous copy,
while the other will form a series of short Okazaki fragments.
2. Sintesis Protein
KOMPONEN:
RNA (RNAm dan RNAt)
Ribosom
Kode Genetik
TAHAPAN:
Transkripsi
Translasi
Kode Genetik
(a) Inisiasi
(c) Terminasi
(b) Elongasi
Perkembangan bioteknologi
Rekayasa Genetika
DNA Rekombinan
Teknik Menggabungkan
Molekul DNA
Kloning Gen
Contoh
DNA Vektor
(Plasmid dari E. coli)
DNA
Enzim restriksi
Enzim Ligase
DNA Asing
Plasmid yang telah
terpotong
DNA Rekombinan
(1)
(2)
Warna putih pada koloni diakibatkan adanya kerusakan pada gen Lac
Z yang disisipi oleh gen X (koloni warna putih berarti sel kompeten
membawa DNA rekombinan / DNA plasmid + gen X)
Warna koloni biru berarti sel kompeten yang tumbuh membawa DNA
plasmid saja (tidak disisipi gen X)