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  • Structural Staining Techniques: Activity 2

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    Abi Diciembre Briones
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    After treating samples with PAS reagent, parts of the cell with high carbohydrate content like mucins, glycogen, and the basement membrane can be observed. PAS staining highlights these molecules by using periodic acid to oxidize carbon bonds into aldehydes, which then react with Schiff's reagent to produce a magenta color. The key differences between Feulgen's reaction and PAS reaction are that Feulgen's specifically stains chromosomal material and DNA using fast green as a counterstain, whereas PAS stains a broader range of carbohydrates and uses Schiff's reagent with a blue counterstain.

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    Trial Upload Staining Techniques

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    Cell Bio

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    After treating samples with PAS reagent, parts of the cell with high carbohydrate content like mucins, glycogen, and the basement membrane can be observed. PAS staining highlights these molecules by using periodic acid to oxidize carbon bonds into aldehydes, which then react with Schiff's reagent to produce a magenta color. The key differences between Feulgen's reaction and PAS reaction are that Feulgen's specifically stains chromosomal material and DNA using fast green as a counterstain, whereas PAS stains a broader range of carbohydrates and uses Schiff's reagent with a blue counterstain.

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    19 views11 pages

    Structural Staining Techniques: Activity 2

    Uploaded by

    Abi Diciembre Briones
    After treating samples with PAS reagent, parts of the cell with high carbohydrate content like mucins, glycogen, and the basement membrane can be observed. PAS staining highlights these molecules by using periodic acid to oxidize carbon bonds into aldehydes, which then react with Schiff's reagent to produce a magenta color. The key differences between Feulgen's reaction and PAS reaction are that Feulgen's specifically stains chromosomal material and DNA using fast green as a counterstain, whereas PAS stains a broader range of carbohydrates and uses Schiff's reagent with a blue counterstain.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pptx, pdf, or txt
    of 11

    Activity 2

    Structural Staining
    Techniques

    9.

    After the samples were treated


    with PAS reagent, what part of the
    cell can be observed? Explain why.

    PAS

    reagent

    Principle: The PAS stain is a

    histochemical reaction in that the


    periodic acid oxidizes the carbon to
    carbon bond forming aldehydes which
    react to the fuchsin-sulfurous acid which
    form the magenta color.

    PAS

    staining is mainly used for staining


    structures containing a high proportion
    ofcarbohydratemacromolecules
    (glycogen,glycoprotein,proteoglycans)
    , typically found in e.g.connective
    tissues,mucus, theglycocalyx,
    andbasal laminae.

    This

    techniquehighlights molecules
    in the cell with high carbohydrate
    content such as mucins, glycogen,
    and the basement membrane.

    1.

    Differentiate Feulgens reaction


    from PAS reaction.

    Technique

    Feulgens Reaction

    PAS Reaction

    Stain

    Fast Green

    Periodic Acid (HCl)

    Counterstain

    Schiffs Reagent

    Schiffs Reagent

    Molecule

    chromosomal
    materialorDNA

    Glycogen, neutral
    mucosubstances,
    basement membranes,
    collagen fibers, glycolipids
    and phospholipids

    Carbon
    Compound

    dialdehydes

    dialdehydes

    Resulting Color

    Magenta against
    Green

    Magenta against Blue

    11.

    Differentiation of blood cells and


    neuron PAS method.

    Neuron cells

    Neuron cells

    Blood cells

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