Biochemistry and medicine enjoy a mutually cooperative relationship.

Biochemical studies have illuminated many aspects of health and disease, and the
study of the many various aspects of health and disease has opened up a new
areas of biochemistry. These close relationships emphasize that life, as we know
it, depends on biochemical reactions and processes (Harpers, 30 th ed., 2015). The
range is very wide, and therefore we need preliminary chemical studies and
certainly more than the detailed knowledge of anatomy. For in many cases, it is
impossible to make diagnosis or providerational treatment without recourse to the
laboratory. Laboratory procedures that includes, the Extraction, Fractionation and
Identification of brain lipids.
The lipids are heterogeneous group of compounds, including fats, oils,
steroids, waxes, and related compounds that are related more by their physical
than by their chemical properties. They have the common property of being (1)
relatively insoluble in water and (2) soluble in non polar solvents such as ether
and chloroform (Harpers, 30th ed., 2015). We can use this solubility difference to
extract and separate lipid constituents from the many polar compounds that are
contained in biological tissues. Quantitative isolation of lipids from tissues in their
native state and free of non-lipid contaminants must be accomplished before
analysis is attempted. Although this first step can be tedious, time-consuming and
relatively uninteresting, any slackness will almost certainly result in the loss of
specific components and in the production of artefacts (W.W. Christie, Advances in
Lipid Methodology 1993). Lipids can be classified by a number of schemes, but
looking at them according to their biological function is most useful. Fatty acids
serves asmetabollic fuel, phospholipids are precursors of second messengers for
signal transduction and cholesterols areprecursors of Vitamin D, bile salts and
steroid hormones. Corelating, adult nervous tissue has high lipid content in
comparison to other tissues. Prior to myelination , the lipid content of fetal brain
resembles that of other organs. However, even in the final stage of development
of the brain, changes in composition continues. There is an increase of total lipid
of whole human brain up to 30 years of age, and thereafter there is a decrease in
total lipid. These observation indicate the continually changing lipid level in the
human nervous system throughout life and emphasis on the dynamic state of
brain lipids.