B Y T

TRNG I HC DC H NI

TRN NGC YN CHI

SNG LC CC CY THUC C
TIM NNG IU TR BNH GT
THNG QUA C CH XANTHIN
OXIDASE IN VITRO
KHA LUN TT NGHIP DC S
Ngi hng dn:
1. T.S. Nguyn Thy Dng
2. T.S. Phng Thin Thng
Ni thc hin:
B mn Dc lc

H NI - 2013

LI CM N
hon thnh kho lun ny, ti nhn c s gip rt nhiu ca
thy c, bn b v ngi thn.
Trc tin ti xin by t lng bit n su sc ti TS. Nguyn Thu Dng
v TS. Phng Thin Thng, nhng ngi thy lun quan tm, gip ,
hng dn v ng vin ti trong sut qu trnh hon thnh ti ny.
Xin chn thnh cm n cc thy, c v cc anh ch k thut vin B mn
Dc lc gip v to iu kin cho ti trong sut qu trnh nghin cu v
thc hin ti.
Xin chn thnh cm n cc cn b phng o to, cc b mn, phng ban
khc ca trng i hc Dc H Ni.
Li cui cng, ti xin gi li cm n n gia nh v bn b, nhng ngi
lun lun ng vin, gip v ng gp kin cho ti hon thnh kho lun ny.
H Ni, thng 5, nm 2013.
Sinh vin

Trn Ngc Yn Chi

MC LC
LI CM N
MC LC
DANH MC CC CH VIT TT V K HIU
DANH MC CC BNG
DANH MC CC HNH
T VN .............................................................................................................1
CHNG 1. TNG QUAN .......................................................................................3
1.1. Vi nt v bnh gt & enzym xanthin oxidase .................................................3
1.1.1. Khi nim v c ch bnh sinh bnh gt....................................................3
1.1.2. Mi lin quan gia bnh gt v enzym xanthin oxidase ............................4
1.1.3. Enzym xanthin oxidase ..............................................................................5
1.2. Mt s thuc v dc liu c tc dng c ch XO...........................................7
1.2.1. Cc thuc c ch XO ang c lu hnh.................................................7
1.2.2. Cc nghin cu v pht trin dc liu c tim nng c ch XO ..............9
CHNG 2: I TNG, PHNG TIN V PHNG PHP NGHIN
CU ..........................................................................................................................14
2.1. Nguyn liu nghin cu ..................................................................................14
2.2. Phng tin nghin cu ..................................................................................14
2.2.1. Ho cht, thuc th ..................................................................................14
2.2.2. Dng c v thit b th nghim .................................................................15
2.3. Ni dung nghin cu.......................................................................................15
2.4. Phng php nghin cu ................................................................................16
2.4.1. Phng php thu thp mu v thng tin cc cy thuc a vo sng lc 16
2.4.2. Phng php chit xut dc liu ............................................................16
2.4.3. Phng php nh gi nh hng ca cc mu th ln hot XO .......17
2.4.4. Phng php x l s liu ........................................................................19
3.1. Kt qu ............................................................................................................20
3.1.1. Kt qu thu thp d liu v cc dc liu a vo sng lc ....................20

3.1.2. Kt qu sng lc tc dng c ch XO in vitro ca cc dc liu ............44

3.1.3. Xc nh IC50 ca cc dc liu tim nng c ch XO............................63
3.2. Bn lun ..........................................................................................................63
3.2.1. V thng tin ca cc cy thuc a vo nghin cu ................................63
3.2.2. V kt qu sng lc tc dng c ch XO in vitro trn a Costar 96 ging
............................................................................................................................64
3.2.3. V kh nng c ch XO ca cy thuc tim nng ...................................65
KT LUN ...............................................................................................................67
1. Kt lun ..............................................................................................................67
2. xut ...............................................................................................................67
TI LIU THAM KHO
PH LC

DANH MC CC CH VIT TT V K HIU

XO

Xanthin oxidase

XDH

Xanthin dehydrogenase

Oxh

Oxy ho

CCTT

Cha c thng tin

HMD

iu ho min dich

HA

Huyt p

DANH MC CC BNG

STT

K
hiu

2.1

3.1

Tn bng
B tr th nghim trong tng ging
Thng tin v cc dc liu sng lc

Trang
18
21

nh hng ca cc dch chit ln hot XO in vitro ti

3

3.2

cc nng 100 g/ml, 50 g/ml, 10 g/ml hn hp phn

45

ng
4

3.3

IC50 ca cc dc liu tim nng c ch XO

63

DANH MC CC HNH
STT K hiu
1

1.1

Tn hnh

Trang

Qu trnh chuyn ho purin trong c th

T VN
T lu, cc sn phm t nhin ng vai tr quan trng trong iu tr bnh.
Trong hai thp k qua c 30,2 % cc loi thuc mi c ph duyt c ngun
gc t t nhin [72]. Cng vi s i mi trong cc phng php iu tr bng cc
sn phm t nhin, cy thuc v cc ch phm chit xut t thc vt c quan
tm nhiu hn bi nhng u im so vi ho dc [114]. Cc thuc theo y hc hin
i thng c tc dng nhanh, hiu qu tng i tt. Tuy nhin, khi dng tn dc
iu tr cc bnh mn tnh li bc l nhc im nhiu tc dng khng mong
mun v gi thnh cao khi dng lu di. Gt l bnh ri lon chuyn ho mn tnh.
Ngi b bnh gt phi i mt vi vic s phi s dng thuc trong thi gian di
v c th gp nhiu vn m cc thuc tn dc mang li. Do , nhiu nghin
cu v thuc c ngun gc t dc liu c a ra khc phc cc nhc
im nu trn.
Bnh gt gn lin vi tng acid uric mu v mc tiu chnh trong iu tr l
lm gim lng acid uric trong mu. Acid uric huyt thanh l sn phm cui cng
ca qu trnh chuyn ho purin qua trung gian l xanthin oxidase (XO), mt loi
enzym oxy ho hypoxanthin thnh xanthin v xanthin thnh acid uric. Vic s dng
cc cht c ch enzym XO cn tr hnh thnh acid uric trong c th l mt mc
tiu m cc nghin cu v ang hng ti. Vi truyn thng lu i v y hc c
truyn, vi nhng kinh nghim dn gian v s dng cy c lm thuc t cc dn tc
khc nhau trong nc, Vit Nam c mt ngun dc liu phong ph v cn cha
c khai thc nhiu. T thc t , gp phn sng lc nhm tm kim cc dc
liu c kh nng c ch XO, bc u trong qu trnh xc nh dc liu tim nng
iu tr gt, ti: Sng lc cc cy thuc c tim nng iu tr bnh gt thng
qua c ch xanthin oxidase in vitro c thc hin vi ba mc tiu sau:
1. Thu thp c thng tin ca mt s cy thuc Vit Nam c tim nng khai
thc.

2. Sng lc c cc cy thuc c tim nng iu tr gt thng qua con ng

c ch xanthin oxidase in vitro trn a 96 ging.
3. Xc nh IC50 ca cc cy thuc c tim nng c ch XO.

CHNG 1. TNG QUAN

1.1. Vi nt v bnh gt & enzym xanthin oxidase
1.1.1. Khi nim v c ch bnh sinh bnh gt
1.1.1.1. Khi nim
Gt l bnh ri lon chuyn ho cc nhn purin, c c im chnh l tng
acid uric mu. Tnh trng vim khp trong bnh gt l do s lng ng cc tinh th
monosolium urat trong dch khp hoc m [9].
1.1.1.2. C ch bnh sinh bnh gt
Bnh gt xut hin khi c th tch lu acid uric qu mc (gp vi ln bnh
thng). Nguyn nhn l do sn xut acid uric qu mc hoc gim thi tr acid uric.
Tng acid uric qu mc c th do s bt thng ca mt s h enzym tc
ng vo qu trnh chuyn ho purin nh tng hot tnh phosphoribosyl
pyrophosphat (PRPP) synthetase dn n tng nng PRPP - cht quan trng
trong qu trnh tng hp purin hay thiu ht hypoxanthin-guanin phosphoribosyl
transferase (HGPRT) - enzym chu trch nhim chuyn guanin thnh guanylic acid
v chuyn hypoxanthin thnh inosinic acid.
Hin tng gim thi tr acid uric thn vi hu ht cc bnh nhn gt (8090%) u khng r nguyn nhn (tng acid uric mu t pht). Thng thng, nu
sn xut acid uric cn bng vi thi tr th acid uric khng b tch ly. Tuy nhin,
bnh nhn gt lng thi tr t hn nhiu so vi lng acid uric sinh ra dn n
tng lng tinh th natri urat ha tan trong mu [36].
Khi nng acid uric tng cao s gy lng ng cc tinh th urat ti m to
nn cc ht tophi. Khi cc ht tophi ti sn khp b v s khi pht cn gt cp, s
lng ng vi tinh th cnh khp, mng hot dch, trong m sn v m xng s dn
n bnh xng khp mn tnh do gt. S c mt vi tinh th urat ti m mm, bao
gn to nn ht tophi; vim thn k l do tinh th urat lng ng ti t chc k ca
thn; acid uric niu tng v s toan ho nc tiu dn n si tit niu [9].

1.1.2. Mi lin quan gia bnh gt v enzym xanthin oxidase

Acid uric l sn phm cui cng ca qu trnh chuyn ho cc nucleoprotein
c cha nhn purin trong c th [1]. Cc purin c to ra chuyn ho thnh
hypoxanthin v xanthin, di tc dng ca enzym XO, chng c oxy ho thnh
acid uric. Qu trnh chuyn ho base purin trong c th c miu t trong hnh
1.1. [36].
Ribose 5-phosphat
synthetase
PRPP
Nucleic
acid

Guanin

iu ha
ngc c ch

Glutamin
iu ha
ngc c ch

Inosinic acid

Nucleic
acid

Adenylic acid
HGPRTase

PRPP

Guanylic
acid
HGPR
Tase

PRPP

Hypoxanthin

Xanthin

PRPP

Xanthin oxidase

Acid uric
Hnh1.1. Chuyn ho purin trong c th.

Bnh gt c mi quan h cht ch vi nng acid uric mu. Khi nng

acid uric mu tng cao s lm tng nguy c mc bnh. Bn cnh , enzym XO c
vai tr ch cht trong qu trnh iu ho nng acid uric mu. Do vy, c ch
enzym XO l mt trong cc c ch chnh m cc thuc iu tr gt ang hng ti.
1.1.3. Enzym xanthin oxidase
1.1.3.1. V tr, c im l ho v c im cu trc
-

V tr phn b
Enzym XO c tm thy nhiu loi ng vt c v, chim, cn trng v vi

khun [11]. Trong cc m ng vt c v, XO c phn b rng ri gan, rut,

thn, phi, tim, no, huyt tng, hng cu v cc m khc, trong gan v rut
hot ng XO l ln nht [21]. ngi XO c tm thy trong hu ht cc t bo
ca c th, tuy nhin n c nhiu nht trong cc t bo gan v cc t bo rut [17].
-

c im l ho
XO c khi lng phn t l 275kDa. N b c ch bi cc ion kim loi

nng, ur cng nh rt nhiu purin, pyrimidin v cc hp cht d vng khc nh

purin-6-aldehyd, 2-amino-4-hydroxypteridin-6-aldehyd Enzym c pH ti u l
4,7 [11]. EDTA, histidin c cho vo qu trnh chit xut enzym bo v enzym
trong sut qu trnh phn ng [84].
-

c im cu trc:
XO l mt protein c 2 tiu n v ging nhau. Mi tiu n v gm 4 trung

tm oxi ho kh 1 Molybdopterin (85kDa), 1 FAD (40kDa) v 2 Fe2S2 (20kDa).

Mo-co bao gm 1 dn xut pterin hu c (molydopterin), c cha 1 chui dithiolen
cng 1 nguyn t Mo [21], [95], [109].
Trnh t aminoacid rt tng ng trong enzym ca loi chut v tng
ng khong 90% gia loi chut vi con ngi [21].

1.1.3.2.Mt s phng php xc nh hot enzym

-

Phng php o quang

+

Phng php o quang da trn lng acid uric to thnh t qu trnh

oxy ha xanthin di xc tc ca XO theo phn ng :

Xanthin + H2O + O2

2 O2

T lng acid to thnh c o bc sng 290nm 25oC, pH = 7,5 hoc

8,0 xc nh c hot XO [93].
+

Phng

php

quang

dng

ABTS

(2,2-azino-bis(3-

ethylbenzothiazolin-6-sulphonat) [79]:
Nguyn tc phn ng:
Hypoxanthin + 2 H2O+ 2 O2
Acid uric + O2

2 O2

alantonin + H2O2 + CO2

H2O2 + ABTSred

ABTSOX + 2 H2O

T lng ABTSOX to thnh o quang bc sng 410 nm xc nh c

hot XO.
-

Phng php o p
xc nh hot enzym c th nh gi s kh ca cc cht nhn

electron l oxy, xanh methylen v cytochromC.

Nguyn tc ca phng php da trn o t l oxi tiu th vi c cht
xanthin, oxy c o p 37oC [35].
-

Mt s phng php khc:

o hunh quang ca isoxanthopterin c to thnh: Hot ng ca XDH v

XO c th c phn tch hunh quang bng cch s dng qu trnh oxy ho pterin
thnh isoxanthopterin nh bin php kch thch hot ng enzym bc sng
345nm v pht x ti 390nm. T l oxy ho pterin c xc nh bi s vng mt
v hin din ca cht nhn electron xanh methylen t nh gi hot ng ca
XDH v XO tng ng [21].

Xc nh hot ng enzym bng cch nh lng H2O2 c to thnh trong

qu trnh hydroxyl ho xanthin bi XO. Flavin c kh phn ng vi O2 to thnh
H2O2 [21].
o oxy tiu th: Phn ng xanthin/XO c nghin cu bng cch o thi
gian lin tc ca oxy s dng bng 1 in cc chn lc [21].

1.2. Mt s thuc v dc liu c tc dng c ch XO

1.2.1. Cc thuc c ch XO ang c lu hnh
Allopurinol v febuxostat l hai thuc in hnh c tc dng c ch XO hin
ang c s dng trong iu tr.
1.2.1.1. Allopurinol
Allopurinol l cht u tin c xc nh c kh nng c ch XO v by
gi vn l thuc iu tr tng acid uric mu thng c s dng.
-

Tc dng v c ch tc dng
Tc dng [3], [24]:
Thuc c ch XO, ngn chn s tng hp urat t hypoxanthin v xanthin do

lm gim lng acid uric trong mu.

Khi khng dng allopurinol, purin thi tr qua nc tiu di dng acid uric.
Khi dng thuc, purin trong nc tiu s tn ti 3 dng: acid uric, hypoxanthin v
xanthin. Mi cht c tan ring nn kt qu l lm tng lng thi tr purin qua
nc tiu, gim nng urat v acid uric huyt tng m khng lm ng niu
b tip xc vi mt lng acid uric qu ln.
Allopurinol lm gim acid uric mu di tan gii hn nn gip ha tan
cc ht tophi v ngn nga s tin trin ca vim khp mn do gt. Ngoi ra, acid
uric mu gim cng lm gim s hnh thnh si, ngn nga tin trin bnh v thn.
C ch:
Allopurinol lm h acid uric mu thng qua c ch enzym XO. Trong qu
trnh c ch enzym, allopurinol c hydro ho thnh alloxanthin (oxypurinol),
chng phi hp cht ch vi cc trung tm Mo b kh to thnh phc hp khng

hot ng [82]. nng thp, allopurinol l c cht v l cht c ch cnh tranh,

nng cao hn, n l cht c ch khng cnh tranh [95].
-

u im
Thuc c dng nhiu hn cc thuc gy tng thi acid uric mu do c th

dng c trn hu ht bnh nhn, dng mt ln mt ngy v t l gp tc dng

khng mong mun t hn [62].
Thuc lm tng bi xut cc tin cht ca acid uric qua nc tiu nn t gy
si thn v cn au qun thn hn [3].
Ngoi iu tr gt, allopurinol cn c s dng phi hp iu tr cc loi
thiu mu cc b, tn thng mch mu v suy tim mn ci thin hot ng bt
thng ca ni m v lm gim cc du hiu stress oxy ho [65], [95].
-

Nhc im
Khung cu trc ca allopurinol v oxypurinol c nhn purin v pyrimidin,

chu trch nhim cho mt s tc dng khng mong mun ca allopurinol [95].
Cc tc dng khng mong mun ph bin nht ca allopurinol l au d dy,
phn ng qu mn v pht ban da [95]. Hi chng qu mn ca allopurinol c
trng bi st, pht ban, vim gan, tng bch cu v suy chc nng thn [69]. Cc
tc dng ny thng xy ra nhng ngi suy gim chc nng thn, cha c
hiu chnh liu allopurinol [95].
1.3.1.2. Febuxostat
Febuxostat ln lt c C quan qun l thuc Chu u - EMEA v
Cc qun l Thc phm v dc phm Hoa K - FDA thng qua iu tr di hn
tng acid uric mn tnh trn bnh nhn gt [18], [23].
-

Tc dng v c ch tc dng:
Febuxostat lm h acid uric mu bng cch c ch chn lc XO. Febuxostat

khng c nhn purin. Khng ging nh oxypurinol ch gn cht vi dng kh XO,

febuxostat gn vi c dng kh v dng oxy ho ca enzym. Febuxostat gn vi
knh di v hp dn n molypden-pterin lm che khut v tr gn ca c cht, t
c ch hot ng ca enzym [47].

u im:
So vi allopurinol, febuxostat t gy d ng hn, hiu lc mnh hn khi la

chn cho bnh nhn khng ung c allopurinol hoc cc thuc iu tr bnh gt
khc. Thuc hu ch vi 5-10% ngi c hin tng qu mn vi allopurinol
cng nh nhng ngi suy thn khng th dng liu allopurinol [107].
Febuxostat khng c purin trong cu trc. Tuy c ch XO ging allopurinol
nhng febuxostat khng c ch cc enzym khc trong con ng chuyn ho purin
v pyrimidin [107], [47]. Do , thuc t gy hin tng qu mn hn allopurinol.
Febuxostat ch yu chuyn ho qua gan. V vy, thuc c th khng cn phi
iu chnh mc liu ngi suy thn nh allopurinol [107].
-

Nhc im
Trong cc nghin cu thc hin, febuxostat an ton hn allopurinol. Tuy

nhin, y l thuc mi, kinh nghim s dng cha nhiu. V vy, cc tng tc
thuc cng nh cc tc dng khng mong mun ca thuc cn phi tip tc c
nghin cu [107].
1.2.2. Cc nghin cu v pht trin dc liu c tim nng c ch XO
Allopurinol v febuxostat l 2 thuc tn dc in hnh trong iu tr gt
mn. Hiu qu iu tr tt l nhng u im ni bt ca hai thuc trn. Tuy nhin,
chng c nhiu tc dng khng mong mun ng lo ngi. V vy, cc dc liu
iu tr gt theo c ch c ch XO vn ang c tip tc pht trin khc phc
nhc im trn.
1.2.2.1. Cc hng nghin cu trn th gii
Nghin cu sng lc in vitro l bc u t c th nghin cu theo
nh hng tc dng sinh hc v nh gi su hn khng nh tc dng trn m
hnh gy bnh trn ng vt thc nghim ca cc dc liu c kh nng c ch XO
mnh nht.
Trn th gii, hng nghin cu sng lc in vitro v dc liu cng nh cc
hot cht t dc liu tm kim ngun thay th vi hiu qu c ch enzym XO
tng t hoc vt tri hn ng thi t gy tc dng ph nghim trng hn

10

allopurinol c quan tm pht trin. Ngy cng c nhiu nghin cu c thc

hin khp cc nc trn th gii nh Australia, Vit Nam, Cng ho Sc,
Paraguay, Panama, Philippin, Trung Quc, n v cho nhng kt qu kh
quan [94], [114], [67], [121], [48], [20], [40], [90].
Lin quan n tc dng c ch XO in vitro, 3 hng tip cn ch yu ca cc
nghin cu c thc hin bi cc nhm nghin cu khc nhau trn th gii v
sng lc dc liu v cc cht/nhm hp cht phn lp t dc liu.
-

Tip cn sng lc da trn kinh nghim s dng dc liu trong dn gian

hoc nn y hc c truyn ca mt s nc.
Hng tip cn th nht kh ph bin v c thc hin nhiu nc trn

th gii. Hng tip cn ny tp trung sng lc cc loi thuc cc h khc nhau da

trn kinh nghim dn gian hoc nn y hc c truyn cc nc t tm ra cc
cy thuc c tim nng nht. Nm 1998, tc dng c ch XO c kho st trn
26 loi thuc 18 h theo truyn thng c s dng iu tr bnh gt v cc triu
chng lin quan ca ngi dn bn a ca vng ng bc Bc M. Khong 88%
cc cy c tm thy c hot tnh c ch 100 g/ml, vi 20% c s c ch ln
hn 50%. Larix laricina c kh nng c ch cao nht l 86,33% [94].
Trong mt nghin cu khc, 28 dch chit t 17 loi thc vt bn a
Australia c kho st, dch chit Stemodia grossa, Eremophila maculata c tc
dng mnh nht vi IC50 < 50 g/ml [114].
Nm 2000, 122 cy thuc dn gian Trung Quc c la chn da trn
hiu qu lm sng v tn s k n iu tr bnh gt, tng acid uric mu v cc
ri lon lin quan khc nh gi tc dng c ch XO. Kong v cng s pht
hin 69 dch chit methanol v 40 dch chit nc c ch trn 50% hot XO
nng 100 g/ml. Kh nng c ch ln nht l dch chit methanol ca cnh l
Cinnamomum cassia (Lauraceae) (IC50 = 18 g/ml), tip n l hoa ca cy
Chrysanthemum indicum (Asteraceae) (IC50 = 22 g/ml) v l Lycopus europaeus
(Lamiatae) (IC50 = 26 g/ml. Vi cc dch chit nc, thn r ca Polygonum
cuspidatum (Polygonaceae) c ch enzym XO mnh nht (IC50 = 38 g/ml). Tuy

11

nhin, trong nghin cu ny, chng vn yu hn nhiu so vi allopurinol (IC50 =

1,06 g/ml) [67].
Nm 2006, tc dng c ch XO c kho st trn 6 dc liu vi 18 dch
chit nc, methanol v hn hp nc-methanol thuc cc h khc nhau ca ngi
dn bn a n . Trong 18 dch chit c 14 dch chit c ch XO 100 g/ml.
Trong c 10 dch chit c ch ln hn 50%. Sau , cc dch chit methanol ca
Coccinia grandis (IC50 = 29,79 g/ml), Datura metel (IC50 = 76,75 g/ml, Strychnos
nux-vomica (IC50 = 6,8 g/ml) v Vitex negundo (IC50 = 78,5 g/ml) c th
nghim in vivo chut [121].
Nm 2010, nghin cu in vitro kh nng c ch XO ca cc cy c s
dng trong dn gian ca Cng ho Sc v khu vc min Trung, ng u c
tin hnh vi 27 cy thuc, gm 84 dch chit ethanol v methylen clorid. Trong
dch chit ethanol - methylen clorid ca Populus nigra v Betula pendula c ch
mnh nht vi IC50 tng ng l 8,3 v 25,9 g/ml. Tip l dch chit 80%
ethanol Caryophyllus aromaticus v Hypericum perforatum c IC50 di 50 g/ml
[48].
Nm 2011, Apaya v cng s nh gi kh nng c ch XO ca dch chit t
15 dc liu c s dng vi tc dng chng vim trong y hc c truyn
Philippin. Kt qu cho thy 5 dc liu c tc dng c ch trn 50% nng 100
g/ml, tuy nhin tim lc yu hn nhiu so vi allopurinol [20].
-

Tip cn sng lc theo h hoc chi thc vt

Hng nghin cu th 2 c mt s tc gi la chn sng lc tc dng

c ch ca cc dc liu thuc mt hoc mt s h in hnh. Hng nghin cu

ny tp trung nghin cu su theo mt h, mt s h hoc chi nht nh xc nh
cy thuc c tim nng nht. Trong dn gian, cc h ny c s dng ph bin vi
tc dng gim au, chng vim.
Nm 1988, mt nghin cu ca Paraguay c tin hnh trn h Sim
(Myrtaceae) trong Hexachlamys edulis l dc liu c ch XO mnh nht vi
IC50 = 5,5 g/ml [118]. Ti Panama, 9 loi ca h Dy gi (Celastraceae) v h Hoa

12

mi (Lamiaceace) c kho st v dch chit ethanol ca Hyptis obtusiflora

(Lamiaceae) v Hyptis lantanaefolia (Lamiaceae) c kh nng c ch cao nht,
khong 40% nng 1 g/ml [44]. Cc dc liu ca Brazil trong chi
Lychnophoras h Cc (Asteraceae) c s dng nh thuc gim au chng vim
trong dn gian c nghin cu. Trong s , Lychnophora trichocarpha,
Lychnophora ericoides, Lychnophoriopsis candelabrumv Lychnophora staavioides
l 4 dc liu c kh nng c ch mnh nht [40].
-

Tip cn sng lc theo nhm hp cht phn lp t dc liu

Hng tip cn th 3 i su vo hp cht trong cc nhm hp cht t nhin

c phn lp t tm ra cht c tc dng c ch mnh nht. Flavonoid,

polyphenol, coumarin l nhng cht c nh gi k lng [75], [31], [26]. in
hnh nht l cc flavonoid vi rt nhiu nghin cu. Trong tng s 2092 flavonoid
t c s d liu, Lim v cc cng s la chn c 2 flavonoid l myricetin v
luteolin c tc dng c ch XO tt vi IC50 tng ng l 62,7 v 3,39 g/ml [72].
Bn cnh , nghin cu v mi lin quan cu trc-tc dng sinh hc ca cc hp
cht flavonoid nh apigenin, quercetin, myricetin, isovitexin, genistein v
naringenin cng c thc hin. Kt qu cho thy, apigenin l cht c ch mnh
nht, tng tc thun li nht vi trung tm hot ng enzym. Tip theo l
quercetin, myricetin, genistein v isovitexin. Naringenin khng cho thy s c ch
vi IC50 > 50 M [75].
1.2.2.2. Cc nghin cu ca cc tc gi Vit Nam
Vi ngun ti nguyn dc liu phong ph v nhng kinh nghim lu i s
dng cy c lm thuc, cc nh khoa hc Vit Nam cng thc hin cc nghin
cu sng lc, bc u cho kt qu kh quan. Nm 2004, Nguyn Th Thanh Mai
v cng s thc hin nghin cu theo nh hng sng lc dc liu c tim
nng c ch XO t ngun dc liu Vit Nam vi 288 dch chit t 96 cy thuc
c s dng trong y hc c truyn. Kt qu cho thy nng 100 g/ml, 46 dch
chit c s c ch ln hn 50% hot tnh enzym. 50 g/ml, 21 dch chit c ch
hn 50%. 25 g/ml, 8 dch chit cho thy c ch hn 50%, trong khi ti nng

13

10 g/ml, 2 dch chit c s c ch ln hn 50%. Cc dch chit methanol ca

Artemisia vulgaris (Ngi cu), Caesalpinia sappan (T mc), Blumea balsamifera
(i bi), Chrysanthemum sinense (Cc hoa) v dch chit methanol-nc ca
Tetracera scandens (Dy chiu) c kh nng c ch XO mnh vi IC50 < 20 g/ml.
Dch chit methanol t hoa Chrysanthemum sinense c kh nng c ch mnh nht
vi gi tr IC50 = 5,1 g/ml [86]. T c s sng lc , cc cy c nghin cu
su hn. T rut g ca cy Caesalpinia sappanphn lp c 17 hp cht t nhin
trong c 9 hp cht c tc dng c ch XO vi IC50 dao ng t 3,9 n 93,2
M. Hp cht c tc dng mnh nht l sappanchalcon vi IC50 = 3,9 M [87].
Nghin cu ho thc vt t cy Chrysanthemum sinense phn lp c mt flavon
glycosid mi l acacetin 7-O-(3-O-acetyl-beta-D-glucopyranoside) v 27 hp cht
bit khc bao gm flavonoid, dn cht ca acid caffeoyl quinic, monoterpenoid
glycosid. Cc hp cht ny c hot tnh c ch XO mnh hn allopurinol (IC50
=2,5 M) vi IC50 dao ng t 0,13-2,31 M [88]. Trong s 8 hp cht c phn
lp t dch chit methanol ca cy Blumea balsamifera c mt dihydroflanovol mi
th hin kh nng c ch mnh nht vi IC50 = 0,23

0,01 M [89]. Gn y nht,

t dc liu Tetracera scandens phn lp c mt hp cht nor-lupan triterpen

mi l 28-O- -D-glucopyranosyl ester ca acid platonic vi kh nng c ch XO
mnh vi IC50 = 1,9 M [90].
Trong khun kh ti ny, hng tip cn th nht s c ng dng
sng lc dc liu c tim nng iu tr gt thng qua c ch XO t ngun dc
liu thu thp t d n Bo tn ngun cy thuc c truyn ca Vin Dc liu. T
, ti hi vng tm ra dc liu c kh nng c ch XO mnh nht v a vo
nghin cu su hn.

14

CHNG 2: I TNG, PHNG TIN V PHNG

PHP NGHIN CU
2.1. Nguyn liu nghin cu
2.1.1. Dc liu nghin cu
Dc liu nghin cu gm 202 cy thuc c thu hi ti Vit Nam theo d
n Bo tn ngun cy thuc c truyn ca Vin Dc liu [4].
2.1.2. Chun b mu nghin cu
Cc mu dc liu sau khi thu hi c ra sch, phi hoc sy kh ri
trong ti polyethylen. Bo qun cc mu trong iu kin kh ro, thong mt,
trnh nh sng, mi mt, mc. Khng s dng ho cht bo qun mu.
Dc liu c xay th, c chit xut thnh dng cn methanol ton phn.
Chun b mu th cho th nghim in vitro: cn c ho tan trong dimethyl
sulfoxid (DMSO), c pha long bng m thnh cc nng 100 g/ml, 50
g/ml, 10 g/ml thc hin cc phn ng enzym.

2.2. Phng tin nghin cu

2.2.1. Ho cht, thuc th
Allopurinol (Sigma Aldrich).
Xanthin

99% (Sigma Aldrich).

Xanthin oxidase (9 n v/mg protein, 8 mg protein/ml, Sigma Aldrich).

Dimethyl sulfoxid (Merck).
Na2HPO4.2 H2O (Vel).
KH2PO4 (Vel).
HCl, NaOH (t tiu chun phn tch).
Cc ha cht v dung mi khc s dng trong nghin cu t tiu chun
dc dng.

15

2.2.2. Dng c v thit b th nghim

My o pH (EUTECH).
My ly tm (HERMLE Z300).
My siu m (Power sonic 405).
H thng my ELISA gm my c khay vi tinh th (Biotek, Hoa K) & my
lc khay (Awareness, Hoa K).
My ct nc 2 ln (Halminton, Hoa K).
Thit b khuy t (Heidolph, c).
Cn phn tch AY 220 (SHIMADZU).
96

y phng Costar 3596 (Corning, Hoa K).

u cn, pipet, micropipet, microtube cc loi.

2.3. Ni dung nghin cu

t c cc mc tiu nghin cu t ra, ti c thit k vi cc
ni dung sau:
Thc hin mc tiu 1: Thu thp c thng tin cc cy thuc a vo nghin
cu.
Xc nh tn khoa hc, b phn dng.
Tra cu thng tin v tc dng ca dc liu da trn cc ti
liu tham kho.
Thc hin mc tiu 2: Sng lc cc dc liu c kh nng c ch XO.
Chun b mu th in vitro.
nh gi kh nng c ch ca cc mu th 3 nng 100
g/ml, 50 g/ml v 10 g/ml.
Thc hin mc tiu 3: Xc nh c IC50 ca cc cy thuc c tim nng c
ch XO.
Kho st kh nng c ch ca cc mu th tim nng cc
nng khc nhau xc nh IC50.

16

2.4. Phng php nghin cu

2.4.1. Phng php thu thp mu v thng tin cc cy thuc a vo sng lc
Thng tin v tn cy, b phn dng, tn khoa hc c xc nh bi nhm
thc hin D n Bo tn cy thuc c truyn. Vic nh danh tn khoa hc ca
mu dc liu do c nhn Ng Vn Tri, Vin Dc liu v mt s chuyn gia v
thc vt xc nh. Mu tiu bn hoc mu cy c lu gi ti Vin Dc liu.
Thng tin v tc dng ca cc cy thuc c thu thp t cc ngun d liu:
sch Nhng cy thuc v v thuc Vit Nam ca Tt Li [10], sch Dc liu
hc tp 2 ca nh xut bn Y hc [2] v cc tp ch khoa hc t ngun c s d liu
Pubmed, Science direct v Wiley.
Mt s dc liu c s dng trong dn gian v y hc c truyn cha c
nghin cu v thnh phn ho hc cng nh hot tnh sinh hc. Trong cng vic
thc hin D n Bo tn ngun cy thuc c truyn, nhm thc hin thu hi mu
cy, lm tiu bn, chp nh, v nh m s dc liu xc nh tn khoa hc. Cc
mu dc liu thu hi c th xc nh ngay c tn khoa hc, cng c th cha
xc nh ngay c tn khoa hc m cn c thi gian tra cu, tham kho ti liu
v cc chuyn gia v thc vt hc. Sau khi xc nh c tn khoa hc th nhm
nghin cu s xem xt c th c bin php bo tn hay pht trin cy thuc.
thi im hin ti, a s cc cy thuc c tn a phng, tn khoa hc, b phn
dng, tc dng, nhng cng c nhng cy thuc cha c nhng thng tin ny.
2.4.2. Phng php chit xut dc liu
Cn 100 g mu dc liu sy kh cho vo bnh cu 1 lt, thm m vi
MeOH ri tip tc MeOH ngp dc liu khong 3 cm. Chit (2 ln) hi lu
cch thu trong 2 gi, lc qua giy lc ly dch chit. C dch chit c cn
MeOH ton phn.

17

Mt s dc liu c 2 mu th l cn methanol t cc b phn khc nhau ca

cng mt cy. Nhm nghin cu cng thu hi mt s mu dc liu cng mt loi
(cng tn khoa hc) nhng c thu hi ti cc a phng khc nhau kho st
s khc nhau ca cc dc liu theo vng min.
2.4.3. Phng php nh gi nh hng ca cc mu th ln hot XO
2.4.3.1. Nguyn tc
XO xc tc cho qu trnh oxy ha xanthin thnh acid uric.
Xanthin + O2 + H2O

Uric acid + H2O2

Hot XO t l vi lng acid uric c nh lng bng phng php o

quang bc sng 290nm.
2.4.3.2. Chun b ho cht v cht th
m phosphat 70 mM, pH = 7,5.
Xanthin oxidase 0,04 U/ml: pha t XO 7 U/ml bng m phosphat.
C cht xanthin 260 M.
Dung dch HCl 1M dng phn ng.
Dung dch allopurinol 0,5 g/ml.
Mu th: Cn methanol ca cc mu cy c ho tan trong dimethyl
sulfoxid (DMSO). Sau cc mu th c pha trong m cc nng
100 g/ml, 50 g/ml, 10 g/ml.
2.4.3.3. Tin hnh
Th nghim c thc hin trn a 96 ging.
B tr trong mt a gm c: mu chng, mu i chiu (allopurinol) v mu
th.
B tr hn hp th trong tng ging c th hin bng 2.1.

18

Bng 2.1. B tr th nghim trong tng ging

Mu chng

Mu i chiu

Mu th

Dung dch th

20l

Allopurinol

20l

85l

65l

65l

Xanthin

60l

60l

60l

XO

30l

30l (sau HCl)

30l

HCl

25l

25l

25l

Tng th tch

200l

200l

200l

Dung dch m
phosphat

Hn hp th bao gm:
20l dung dch th.
65l dung dch m phosphat 70mM, pH = 7,5.
30l dung dch enzym (0,04 U/ml trong dung dch m phoshat
70mM pH = 7,5) c chun b ngay trc khi s dng.
Sau khi 25oC trong 15 pht, thm 60l xanthin 260M ri
25oC trong 30 pht.
Phn ng ngng li bng cch cho thm 25l HCl 1N v o hp
th 290nm.
Mu trng ca chng, i chiu v cc mu th cng c chun b
song song nh trn nhng enzym c thm vo sau HCl 1N.
Mi mu c th 3 nng 100 g/ml, 50 g/ml, 10 g/ml (trong ging).
Mi nng c tin hnh th 3 ln, ng vi mi nng tnh c 3 gi tr
OD v tnh trung bnh % c ch t 3 gi tr o c.

19

Tc dng c ch enzymXO c tnh theo cng thc [6], [104].

OD chng = ACT-AC ; OD th = AST-AS
Phn trm c ch XO = I% = [1 OD th / OD chng] x 100
Trong : AC l hp th ca mu chng
ACT l hp th ca mu trng ca chng
AS l hp th ca mu th
AST l hp th ca mu trng ca th.
2.4.3.4. Xc nh IC50 ca cc cy c tim nng c ch XO
Tc dng c ch XO ca cc cy c la chn c nh gi theo tr s
IC50, l gi tr nng (tnh ton theo l thuyt) ti mu th c ch 50% s to
thnh acid uric trong hn hp phn ng so vi mu chng [5].
Vi tng mu th c la chn, thit lp dy nng kho st t xc
nh IC50 v gii hn khong tin cy.
2.4.4. Phng php x l s liu
S liu c d tr bng phn mm Microsoft Excel Mac 2011 v tnh trung
bnh I% cc nng 100 g/ml, 50 g/ml, 10 g/ml bng Microsoft Excel Mac
2011. Gi tr OD dc biu din di dng M

SD (M: gi tr OD trung bnh

tng mu, SD: lch chun), so snh gi tr trung bnh ca cc mu bng one-way
ANOVA, dng hu kim Dunnett test so snh gi tr trung bnh ca OD mu
th so vi mu chng. Tnh IC50 v khong tin cy 95% ca IC50 bng phng php
phn tch hi quy probit trn phn mm SPSS 16.0.

20

CHNG 3. KT QU V BN LUN
3.1. Kt qu
3.1.1. Kt qu thu thp d liu v cc dc liu a vo sng lc
Thu hi c 202 mu cy ti Vit Nam theo d n Bo tn ngun cy
thuc c truyn ca Vin Dc liu.
Thng tin v tn a phng, tn khoa hc, h, b phn dng v tc dng
dc l c th hin trong bng 3.1.
Nhn xt:
202 mu cy thuc 74 h v 187 loi, trong :
-

27 mu cy cha c thng tin v h.

81 mu cy cha c thng tin v tc dng.

13 mu cy cha c thng tin v b phn dng.

20 mu cy t 10 loi thu hi cc ni khc nhau (ph lc 3.1).

10 mu cy khc b phn dng t 5 loi (ph lc 3.2).

Mt s mu ch c m s, cha c thng tin v tn cy, h, b phn dng v

tc dng.

21

Bng 3.1. Thng tin v cc dc liu c sng lc

STT

Tn ph thng

Tn khoa hc

H KH

B phn
dng

Tc dng

Cc cy thuc thu hi ti vn quc gia Bch M, tnh Tha Thin Hu

1

Tm gi nghin

Vaccinium bullatum

Ericaceae

Thn

CCTT

Mng lng

CCTT

CCTT

CCTT

CCTT

Thanh long

Hylocerus andatus

Castaceae

thn

Xuyn

Cudrania tricuspidata Carr. Bur

Moraceae

cnh, l

Vng cch

Premna corymbosa Rottl. ex Willd

Verbenaceae

cnh l

CT42

Cu ng

Uncaria acida Hunt. Roxb.

Rubiaceae

CT22

Me rng

Phyllanthus emblica L.

Euphorbiaceae

CT40

Gai

Bohmeria nivea L. Gaud.

Urticaceae

cnh c
mc
trn mt
t
l

chng oxh
[19]
chng vim,
oxh [129]
thp khp
[61]
CCTT
h HA[10]
r:chng
vim [74]

22

B phn

STT

Tn ph thng

Tn khoa hc

H KH

CT26

Kim cang qu to

Smilax megacarpa A.DC.

Smilacaceae

thn r

Bo sm

Hibiscus sagistifolius

Malvaceae

thn r

Myrtaceae

10

11

CT29

12

Sn thuyn

Syzygium resinosum (Garnep.) Merr.

et Perry

dng

Tc dng
CCTT
bo v d
dy [13]
khng khun
[7]

T bi bin

Vitex trifolia L f.

Verbenaceae

cnh, l

gim au[10]

13

CT03

An in lng

Hedyotis hirsuta Spreng.

Rubiaceae

c cy

CCTT

14

CT34

V hng dai

Stemonurus coriaceus Miers

Icacinaceae

cnh, l

CCTT

15

CT33

Thi t

Paederia consimilis Pierre ex Pitard

Rubiaceae

cnh l

CCTT

16

CT35

Kinh gii r

Elsholtzia penduliflora W.W. Smith

Lamiaceae

17

CT01

Cu tri, Can tri

Phyllanthus chamaepeuce Ridl.

Euphorbiaceae

ton cy

CCTT

18

CT43

Bch b

Stemona tuberosa Lour.

Stemonaceae

r, l, thn

ho [10]

19

CT18

C 2 l

Solanum diphyllum L.

Solanaceae

ton cy

20

CT21

CCTT

Sp

Acanthaceae

CCTT

trn mt
t

CCTT

chng oxh
[51]
CCTT

23

B phn

STT

Tn ph thng

Tn khoa hc

H KH

21

CT25

Bt ch lng

Glochidion eriocarpum Champ.

Euphorbiaceae

cnh, l

ph thn [10]

22

CT04

CCTT

CCTT

CCTT

CCTT

CCTT

23

CT41

Lng mc ng

Wrightia dubia Spreng.

Apocynaceae

24

CT16

L men

Mosla dianthera Maxim.

Lamiaceae

ton cy

25

CT15

Tm phong

Cardiospermum halicacabum L.

Sapindaceae

ton cy

26

CT34

V hng dai

Stemonurus coriaceus Miers

Icacinaceae

cnh, l

CCTT

27

CT13

Pht d mnh

Dracaena gracilis Wall. ex Hook.f.

Dracaenaceae

CCTT

28

CT17

B my

Clerodendrum cyrtophyllum Tucz

Verbenaceae

trn mt

st pht ban

[10]

29

CT44

Cu ng l hnh tim

30

CT10

Kim cang poilane

Smilax poilanei Gagnep.

Smilacaceae

thn r

31

CT06

Lin ng hoa nh

Illigera parviflora Dunn

Hernandiaceae

CCTT

Uncaria cordata
Lour. Merr.

Rubiaceae

dng

on thn
c mc

Tc dng

c tb ung
th [91]
RLTH [10]
chng vim,
oxh [52]

CCTT
CCTT
cy tm Dao
[12]

24

STT

Tn ph thng

Tn khoa hc

H KH

32

CT19

Bng bong

Lygodium scandens

Lygodiaceae

33

CT36

Mua b

34

CT07

Kim cang

35

CT08

H th trng

36

CT11

L khi

37

CT32

Xun hoa

38

CT51

Bm bc campuchia

39

CT02

An in

Melastoma sanguineum
Sims
Smilax corbularia Kunth
Streptocaulon juventas
Lour. Merr.
Ardisia gigantifolia Stapf
Pseuderanthemum
bracteatum
Mussaenda cambodiana Pierre ex
Pitard
Hedyotis hedyotidea
DC. Merr.

B phn
dng
dy thn
mang l

Tc dng

CCTT
qu: chng

Melastomataceae

cnh, l

Smilacaceae

thn, r

Asclepidaceae

thn r

Myrsinaceae

Acanthaceae

CCTT

cnh, l,

hoa: li tiu

hoa

[10]

Rubiaceae

Rubiaceae

ton cy

oxh [41]
chng vim
[105]
c tb ung
th [120]
c tb ung
th [76]

chng vim
[99]

25

B phn

STT

Tn ph thng

Tn khoa hc

H KH

40

CT38

Lu

Psychotria rubra Poir.

Rubiaecae

thn, l

41

CT31

Bm bc hoa dy

Mussaenda densiflora Li

Rubiaceae

cnh l

42

CT09

Chng chng

Smilax perfoliata Lour.

Smilacaceae

thn r

43

CT28

Cch lng vng

Premna fulva Craib

Verbenaceae

cnh, l

dng

Tc dng
c tb ung
th [49]
CCTT
cha thp
khp [97]
cha vim
khp [92]
chng thiu

44

CT52

Quyn b

Selaginella uncinata Desv. Spring

Selaginellaceae

ton cy

oxy huyt
[138]

45

CT48

An in trng

Hedyotis ovata Thwaites ex Hook.f.

Rubiaceae

cnh, l

46

CT30

Thng sn

Dichroa febrifuga Lour.

Hydrangeaceae

47

CT27

Song giam

Leptonichia acuminatus Mast.

Sterculiaceae

CCTT

48

CT37

Mua lng

Melastoma saigonense Merr.

Melastomataceae

cnh, l

CCTT

CCTT
r: chng
vim [30]

26

STT

Tn ph thng

Tn khoa hc

H KH

B phn
dng

Tc dng

Cc cy thuc thu hi ti Sapa, tnh Lo Cai

49

CT14

Hng bi

Dianella ensifolia L. DC.

Liliaceae

CCTT

50

CT49

Kim cang l bc to

Smilax bracteata Presl

Smilacaceae

thn , r

51

MC8

Kim tuyn Sapa

Anoectochilus roxburghii Wall.

Orchidaceae

ton cy

52

CT47

D cm

53

MC1

Cy xuyn l

Cancora petelotii

54

MC4

Tai th

55

CT50

56

c [10]
chng oxh
[136]
chng oxh
[32]

phn trn

chng oxh

mt t

[16]

Gentiliaceae

ton cy

CCTT

Dipterocarpus sp.

Dipterocarpaceae

qu

Mc chm

Wrightia laevis Hook.f.

Appocynaceae

cnh, l

CCTT

MC2

Trm tu

CCTT

CCTT

CCTT

CCTT

57

CT23

M t t hoa

Rhynchodia pauciflora Pitard

Apocynaceae

cnh, l

CCTT

58

CT20

Gi hc bng

Leea aequata L.

Leeaceae

CCTT

59

MC7

Thch sng hoa tm

Cynoglossum zeylanicum

Boraginaceae

c cy

CCTT

Hedyotis capitellata var. mollis

T.N.Ninh

Rubiaceae

chng vim
[130]

27

STT

Tn ph thng

Tn khoa hc

H KH

60

MC3

Mi chng

Chinacanthus nutans

Acanthaceae

61

S19

H kh tho

Prunella vulgaris L.

Lamiaceae

62

CT58

Ch ch

Parashorea chinensis Wang Hsie.

63

CT53

Thi thanh

B phn
dng
cnh, l

Tc dng
chng oxh
[132]

cm hoa

h huyt p

qu gi

[10]

Dipterocarpaceae

qu

CCTT

Alangium kurzii Craib.

Alangiaceae

thn, l

CCTT

Cc cy thuc thu hi ti Ba V, thnh ph H Ni

64

CT55

Tiu ba v

Piper bavinum C.DC.

Piperaceae

65

MC6

M mt

Isotoma longiflora

Lobeliaceae

66

CT54

Chi mi gn lm

Antidesma montanum Bl.

Euphorbiaceae

67

CT56

Vt vng nht

Viburnum lutescens Bl.

Caprifoliaceae

phn trn
mt t
phn trn
mt t
thn, l
phn trn
mt t

CCTT

CCTT
chng vim
[73]

CCTT

28

STT

Tn ph thng

Tn khoa hc

H KH

68

CT60

L ni

Hydnocarpus kurzii Warb.

Flacourtiaceae

69

CT57

C di hoa trng

Solanum torvum Swartz.

Solanaceae

70

CT61

Cm chy

Sambucus javanica Reinw. Ex Blume

Caprifoliaceae

Lc lc l i

Crotalaria assamica Benth

Fabaceae

71

B phn
dng
phn trn
mt t
thn, l
cnh mang
l, hoa

Tc dng

CCTT
tr nt n[10]
li tiu [10]

cnh mang

chng vim

[66]

phn trn

72

S12

Thc lp l ln

Desmodium sequax Wall.

Fabaceae

73

CT64

Afzelia xylocarpa Craib.

Fabaceae

ht

CCTT

74

CT65

T chu lng d hnh

Callicarpa heterotricha Merr.

Verbenaceae

cnh, l

CCTT

75

CT62

Mi liu

Miliusa balansae Fin. & Gagnep.

Annonaceae

cnh, l

76

CT59

Mu ch l nh

Knema globularia Warb.

Myristicaceae

ht

CCTT

77

S 6

CCTT

CCTT

CCTT

CCTT

CCTT

mt t

CCTT

c tb ung
th [55]

29

B phn

STT

Tn ph thng

Tn khoa hc

H KH

78

CT79

T kn ci

Helicteres hirsuta Lour.

Sterculiaceae

79

S17

Ban l nh

Hypericum uralum Thumb.

Hypericaceae.

80

CT84

T chu

Callicarpa rubella

Verbenaceae

ton cy

81

S 8

Duc c

Derris pachyloba

Fabaceae

thn, l

CCTT

82

S 2

Gic mc t gn

Cornus oligophlebia Merr.

Cornaceae

cnh, l

CCTT

83

CT85

Trm thon

Sterculia lanceolata Cav.

Sterculiaceae

thn

84

S 13

Cu ng cnh leo

Uncaria scandens Smith Hutch.

CCTT

85

S 3

Hoa khoe cy nho gc

Sabia parviflora Wall. ex Rox

Sabiaceae

86

S 14

Tiu rng

Piper sp.

Piperaceae

dng
l
cnh non,
l

on cnh
c mc
r

Tc dng
c tb ung
th [29]
CCTT
chng ung
th [46]

tr sng
ty[10]
CCTT
chng
vim[112]

trn mt

chng oxh

[15]

30

B phn

STT

Tn ph thng

Tn khoa hc

H KH

87

S 5

Cm

Itoa orientalis

Flacourtiaceae

88

S 4

Cm chy

Sambucus javanica Reinw.

Caprifoliaceae

cnh, l

Cam ni

Toddalia asiatica L. Lamk.

Rutaceae

cnh, l

89

dng

90

CT46

Cu ng l ln

Uncaria macrophylla Wall. ex Roxb.

Rubiaceae

cnh, l

91

S 1

Nc nc

Oroxylum indicum

Bignoniaceae

v thn

92

S 20

Lc lc l i di

Fabaceae

cnh, l

93

CT87

Thn linh l to

Apocynaceae

thn, l

94

S 15

Dm dng hoc

Berbridaceae

c cy

95

S 11

Mt gu

Lamiaceae

CCTT

Crotalaria assamica
Benth
Kibatalia macrophylla Woodson
Epimedium macranthun Mooren et
Decne.
Rhabdosia sp.

Tc dng
chng nm
[117]
li tiu [10]
chng oxh
[110]
gin mch
[125]
chng d ng
[10]
r: chng
vim [66]
CCTT
chng vim
[133],[10]
CCTT

31

B phn

STT

Tn ph thng

Tn khoa hc

H KH

96

S 18

Ban trn

Hypericum patulum Ham. Ex. D.Don

Hypericaceae

97

CT80

Rung

Mallotus apelta Muell.-Arg.

Euphorbiaceae

r, thn

98

CT68

Chn

Microdesmis casearifolia

Pandaceae

thn, l

CCTT

99

CT86

Cm lng

Eleaocarpus nitidus Jack

Eleaocarpaceae

CCTT

100

CT75

Mung trung

Zanthoxylum avicennae DC.

Rutaceae

thn, l

101

CT81

Si trng

Sapium sebiferum Roxb.

Euphorbiaceae

cnh, l

102

BV55

Ma d

Costus speciosus Koenig Sm.

Costaceae

thn r

103

BV56

V ch

Ficus heterophyllus L

Moraceae

thn, l

104

CT72

ngn

Cratoxylum pruniflorum Kurz

Hypericaceae

cnh, l

dng

Tc dng

cnh mang

c tb ung

l, hoa

th [123]
bo v gan
[128]

chng vim
[27]
chng vim,
oxh [42]
chng oxh
[37]
cha phong
thp [10]
CCTT

32

STT

Tn ph thng

Tn khoa hc

H KH

B phn
dng

Tc dng
r: khng vi

105

CT88

a bp b

Ficus nervosa

Moraceae

thn, l

khun lao
[28]
c tb ung

106

CT67

Mu ch thanh

Knema elegans Warb.

Myristicaceae

ht

107

CT83

Gi hc

Leea rubra Blunne

Leeaceae

108

CT66

Chi mi m

Antidesma ambiguum Pax & Hoffm

Euphorbiaceae

109

CT76

V san l di

Osmanthus pedulculatus Gagnep.

Oleaceae

cnh, l

CCTT

110

CT77

CCTT

sp1

Magnoliaceae

CCTT

CCTT

111

CT82

Ca l bc

Lauraceae

cnh, l

CCTT

112

CT69

Chn

Pandaceae

thn, l

CCTT

113

CT74

Chi mi

Caryodaphnosis tonkinensis AiryShaw

Microdesmis casearifolia Planch. ex
Hook
Antidesma ghaesembilla Gaertn

Euphorbiaceae

cnh non,
l

cnh non,
l

th [34]
h HA [22]
CCTT

CCTT

33

STT

Tn ph thng

114

CT71

Xoan nh

115

CT70

i khoai

116

BV52

117

Tn khoa hc
Choerospondias axillaris Burtt. &

H KH

B phn
dng

Anacardiaceae

v thn

Aidia oxyodontaYamazaki

Rubiaecae

CCTT

Thin nin kin

Homalomena occulta Lour schott

Araceae

thn r

BV49

Long no

Cinnamomum camphora

Lauraceae

118

CT78

Kim cang l mng

Smilax riparia A.DC.

Smilacaceae

thn r

119

BV34

Con kh

Acanthaceae

120

BV60

Ba kch

Rubiaceae

thn r

Hill

Pseuderanthemum palatiferum Nees

Radlk
Morinda officinalis How

Tc dng
qu: chng
oxh [124]
CCTT
chng oxh
[139]
chng vim,
oxh [71]
gin ph
qun [113]
CCTT
chng oxh
[134]
chng vim,

121

T12-11

C the

Centipeda minima L A.Br.et Aschers

Asteraceae

ton cy

oxh [54],
[73]

34

B phn

STT

Tn ph thng

Tn khoa hc

H KH

122

BV48

C gi t

Balanophora sp.

Balanophoraceae

cnh, l

123

BV47

Hong lin

Coptis teeta Wall

Ranumculaceae

thn r

124

BV51

C gi t

Balanophora sp.

Balanophoraceae

125

BV42

Rt

Hex godajam Colebr. ex. Well.

Hicaceae

126

BV44

Liu l (hng ch)

Ocimum basilicum L.

Lamiaceae

127

BV40

Rt

Hex godajam Colebr. ex. Well.

128

BV0

Cm rng

129

BV58

130

Bv30

dng

phn trn
mt t

Tc dng
chng oxh
[126]
khng khun
[10]
chng oxh
[126]

CCTT

l, cnh

chng vim

mang hoa

ty [10]

Hicaceae.

CCTT

Ehretia acuminata

Boraginaceae

cnh, l

CCTT

Chui ht

Musra barjoo Sieb.

Musaceae

qu xanh

CCTT

Thc lp

Tadehagi triquetrum L.

Fabaceae

phn trn

tr giun

mt t

[127]

35

B phn

STT

Tn ph thng

Tn khoa hc

H KH

131

BV24

L khi

Ardisia silvestris

Myrtaceae

132

CT73

Thu mt Balansa

Bridelia balansae

Euphorbiaceae

thn, l

133

BV59

C ry

Houltuynia cocdata Thumb

Saururaceae

thn r

CCTT

134

CT24

Hng b di

Clausena excavata Burm.f.

Rutaceae

cnh, l

HMD [81]

135

BV36

Khm p

Ixora spp.

CCTT

cnh, l

136

BV35

C dm

Stephania dielsiana Y.C.Wu

Menispermaceae

CCTT

137

BV39

Chc mu

Salacia cochinchinensis

Celastraceae

thn r

tr T [8]

138

T12_12

Mi ch nhiu

Ammania baccifera L.

Lytraceae

CCTT

CCTT

139

T12_02

T xanh

Cassytha filiformis L.

Lauraceae

ton cy

140

BV57

V ch

Ficus heterophyllus L.

Moraceae

dng

Tc dng
cha au d
dy [45]
long m
[111]

khng khun
[70]

chng oxh
[103]

phn trn

cha phong

mt t.

thp [10]

36

B phn

STT

Tn ph thng

Tn khoa hc

H KH

141

T12_09

Lc tin

Passiflora foetida L.

Pasihoraceae

142

T12_03

Sm lng

Eurya ciliata Merr L.

Theaceae

143

BV33

Ventilago leiocarpa Benth.

Rhamnaceae.

144

T12_01

Ci xay

Abutilon indicum L Sweet

Malvaceae

145

T12_07

N qu trng

Flueggea virosa Roxb ex Willd Voigt

Euphorbiaceae

146

BV28

C bit toan

CCTT

CCTT

CCTT

147

BV26

Cm ru(qu xanh)

Glycosmis pentaphylla Correa

Rutaceae

cnh, l

148

T12_05

Chm rng

Strobilanthes cystolithigera Lindau

CCTT

cnh, l

149

BV17

Xui lim(r r)

Homonoia riparia Lour.

Euphorbiaceae

r , g, l

Tm kho my
(dy ct kh)

dng
phn trn
mt t
Cnh, l

Tc dng
an thn [2]
chng long
xng [116]

phn trn

chng vim

mt t

[25]

cnh l

chng vim

mang qu

[119]
chng co git
[98]
CCTT
bo v gan
[85]
CCTT
chng st rt
[91]

37

STT

Tn ph thng

Tn khoa hc

H KH

B phn
dng

Tc dng
thuc tm

150

T12_04

Dy sm ni cao

Cyclea fansipanensis Gagnep

Menispermaceae

thn r

ngi Dao
[12]

151

BV37

Ma d

Costus speciosus Koenig Sm.

Costaceae

thn

chng oxh
[37]

Cc cy thuc thu hi ti Vn quc gia Phong Nha - K Bng, tnh Qung Bnh
152

BV01

Sm cau

Curculigo orchioides Gaertr

Hypoxidaceae

thn r

153

BV02

a lin

Kaempferia galanga L.

Zingiberaceae

thn r

154

BV04

Cm rng l nhn

Ehretia acuminata

Boraginaceae

cnh, l

155

BV05

Thc lp

Desmodium rodgeri Schindl. Ohashi

CCTT

cnh, l

156

BV10

m m hoa trng

Rubus leucanthus Hance

CCTT

cnh, l

chng vim
[68]
chng vim
[122]
CCTT
tr giun
[127]
CCTT

38

B phn

STT

Tn ph thng

Tn khoa hc

H KH

157

BV11

Gai

Boehmeria nivea L. Gaud.

Urticaceae

158

BV12

Chn chim

Scheffena octophylla Lour

Araliaceae

159

BV13

Si rng

Sarcandra glabra Thunb. Nakai

CCTT

160

BV15

Ngy hng

Rubus cochinchinensis Tratt.

CCTT

thn

161

BV16

Ry leo

Pothos scandens L.

Araceae

thn, l

162

BV19

X en

Ehretia asperrula Zoll & Mor

Boraginaceae

163

BV20

Li tin

Stephania longa Lour.

CCTT

dng
l

Tc dng
r: chng
vim [74]

thn, r v

h ng

huyt [10]

phn trn

chng oxh

mt t

[57]

thn, cnh,
l

vim gan
[10]
chng oxh
[106]
chng oxh
[77]

dy thn,

chng vim

[135]

39

B phn

STT

Tn ph thng

Tn khoa hc

H KH

164

BV21

Ngng chan hy( kh rng)

Rourea minor Gaertn Leenh.

Connaraceae

165

BV22

Ng v Nam

Kadsura heteroclita Roxb. Craib

CCTT

thn

166

BV06

Kng ba (Dy m g)

Limacia scandes Lour.

Menispermaceae

thn, l

dng

Tc dng

cnh non,

chng st rt

[50]
chng oxh
[131]
chng trm
cm [64]
cha au

167

BV25

Khi ta

Ardisia silvestris Pitard

CCTT

bng,d dy
[45]

168

BV29

Ba gc

169

BV31

Bm bc

170

BV38

Thin nin kin

171

BV41

Mu ngi

Rauvolfia verticilata Lour. Bail

Apocynaceae

CCTT

thn, l

Homalomena occulta Lour schott

Araceae

thn r

Mucuna pruriens L. DC.

CCTT

thn

Mussaenda cambodiana Pierre ex

Pitard

H HA [2]
hoa: li tiu
[10]
chng oxh
[139]
chng oxh
[14]

40

B phn

STT

Tn ph thng

Tn khoa hc

H KH

172

BV43

C hi

Solanum erianthum D.Don

Solanaceae

173

BV50

T tn

Asarum balansae Franch.

CCTT

174

BV53

B khai

Erythropalum scandens Blume

CCTT

c cy

175

BV61

Sm cau l rng

Curculigo latifolia Dryand. ex Ait.

CCTT

thn r

dng

Tc dng

cnh l

khng khun,

mang hoa

c tb [38]

phn trn
mt t

CCTT
chng vim
[83]
i tho
ng [59]
cy tm

176

T12_08

Cao cng

Ophiopogon reptans Hook

Convallariaceae

thn r

ngi Dao
[12]

177

T12_10

Sn kim

178

CT92

Sang trng

Anisomeles indica
L. Kuntze
Ardisia gracillima

Lamiaceae

Myrsinaceae

trn mt

chng vim

[104]

trn mt
t

CCTT

41

STT

Tn ph thng

Tn khoa hc

H KH

B phn
dng

Tc dng
chng vim

179

CT97

Mung trung

180

CT97

Mc chim

181

T12_13

Xo tam phn

182

BV03

183

Zanthoxylum avicennae DC.

Rutaceae

CCTT

[27]

Apocynaceae

cnh l

CCTT

Paramygnya petebotii Guill.

Rutaceae

cnh, l

CCTT

Si nht

Chloranthus japonicas Sieb

Chloranthaceae

BV07

C thp bt

Equisetum diffusum D.Don

CCTT

184

BV08

a sn

Ilex glomerata King

CCTT

cnh, l

CCTT

185

BV09

Xun hoa

CCTT

cnh, l

h HA [63]

186

BV14

C ry xanh

phn trn

chng vim

mt t

[43]

Amalocalyse miceolobus
Pierre ex Spire

Pseuderanthemum palatiferum Nees.

Radlk.
Iodes cirrhosa Turcz.

Menispermaceae

phn trn
mt t

c tb ung
th [39]
li tiu [60]

42

B phn

STT

Tn ph thng

Tn khoa hc

H KH

187

BV18

Chm ta

Strobilanthes mucronatoproductus

CCTT

cnh, l

CCTT

188

BV23

M hoa

Clerodendrum paniculatum L.

CCTT

cnh, l

CCTT

189

BV27

R qut

Belamcanda chinensis L. DC.

CCTT

thn r

190

BV32

Bng bong do

Lygodium flexuosum L. Sw.

CCTT

c cy

191

BV46

Da di

Pandanus tinctorius

Pandaceae

thn

192

CT90

Ba chc

Euodia lepta

Rutaceae

cnh l

193

CT91

Li tru choi

Tabernaemontana buffalina

Apocynaceae

194

CT93

C sa to l

Euphorbia hirta L.

Apocynaceae

195

CT94

Xng m nguyn

Carallia brachiata

Rhizophoraceae

dng

phn trn
mt t
phn trn
mt t
cnh, l

Tc dng

chng oxh
[58]
chng
vim[33]
gim m
mu [137]
CCTT
CCTT

CCTT
c ch XO
[100]

43

STT

Tn ph thng

Tn khoa hc

H KH

B phn
dng

Tc dng
chng oxh

196

CT95

Thu kn l hp

Helicteres angustifolia

Sterculiaceae

cnh, l

[53], chng
vim [73]

197

CT96

Dy au xng

Tinospora sinensis

Menispermaceae

cnh l

HMD[80]

198

CT99

Tu hoa

Genianthus laurifolius

Asclepidaceae

cnh, l

CCTT

199

CT101

Chy hoa sng

Strobilanthes apricus

Acanthaceae

cnh, l

CCTT

200

CT102

Alseodaphne sp.

Lauraceae

v thn

CCTT

201

CT103

Bi bung

Acronychia pedunculata

Rutaceae

cnh, l

202

CT104

Bp lng

Actinodaphne pilosa Merr.

Lauraceae

cnh, l

Ch thch: CCTT: Cha c thng tin, oxh: oxy ha, tb: t bo, HMD: iu ha min dch

c ch COX2 [96]
CCTT

44

3.1.2. Kt qu sng lc tc dng c ch XO in vitro ca cc dc liu

p dng m hnh vi 3 mc nng : 100 g/ml, 50 g/ml, 10 g/ml
cho tt c dch chit ca cc mu th nh gi nh hng ln hot XO in
vitro.
Kt qu c th hin trong bng 3.2.
Nhn xt:
-

Ti nng 100 g/ml c 20 mu c tc dng c ch XO, c 1 mu c

ch > 50%.

Ti nng 50 g/ml c 13 mu c tc dng c ch XO, khng c mu

no c ch > 50%.

Ti nng 10 g/ml c 3 mu c tc dng c ch XO, khng c mu

no c ch > 50%.

45

Bng 3.2. nh hng ca cc dch chit ln hot XO in vitro ti cc nng 100 g/ml, 50 g/ml, 10 g/ml hn hp phn ng
% c ch XO
STT

Tn khoa hc

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

Vaccinium bullatum

0,544

0,038

6,10

0,393 0,024

6,87

0,406 0,071

6,88

CCTT

0,546

0,01

5,69

0,429 0,026

0,446 0,081

Hylocerus andatus

0,489

0,029

15,5

0,396 0,026

6,10

0,434 0,085

0,458

Cudrania tricuspidata (Carr.) Bur

0,577

0,023

0,35

0,410 0,033

2,38

0,381 0,098

12,6

0,513

0,029

11,4

0,372 0,048

11,43

0,377 0,092

13,5

Premna corymbosa Rottl.Ex

Willd

Uncaria acida Hunt. Roxb.

0,507

0,032

12,4

0,410 0,035

2,38

0,411 0,103

5,73

Phyllanthus emblica L.

0,53

0,04

8,46

0,413 0,026

1,67

0,447 0,085

Bohmeria nivea L. Gaud.

0,454

0,054

21,6

0,400 0,028

4,76

0,41 0,08*

5,96

Smilax megacarpa A.DC.

0,494

0,026

14,7

0,414 0,027

1,43

0,393 0,076

9,86

10

Hibiscus sagistifolius

0,446

0,058

22,9

0,419 0,033

0,238

0,434 0,079

0,458

46

% c ch XO
STT

11

Tn khoa hc

Syzygium resinosum Garnep.

Merr. et Perry

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

0,54

0,007

6,73

0,426 0,036

0,447 0,096

12

Vitex trifolia L f.

0,564

0,028

2,59

0,433 0,030

0,41 0,089

5,96

13

Hedyotis hirsuta Spreng.

0,589

0,023

0,440 0,027

0,438 0,085

14

Stemonurus coriaceus Miers

0,485

0,089

16,2

0,411 0,030

2,14

0,447 0,094

0,518

0,011

10,5

0,451 0,051

0,394 0,096

9,63

0,544

0,022

6,04

0,438 0,018

0,398 0,098

8,71

15

16

Paederia consimilis Pierre ex

Pitard
Elsholtzia penduliflora W.W.
Smith

17

Phyllanthus chamaepeuce Ridl.

0,563

0,021

2,76

0,428 0,024

0,43 0,083

1,37

18

Stemona tuberosa Lour.

0,533

0,054

7,94

0,409 0,016

2,62

0,422 0,085

3,21

19

Solanum diphyllum L.

0,543

0,023

6,21

0,424 0,023

0,462 0,089

20

Sp

0,544

0,002

6,03

0,412 0,022

1,90

0,445 0,079

21

Glochidion eriocarpum Champ.

0,493

0,027

14,8

0,414 0,032

1,43

0,405 0,081

7,11

47

% c ch XO
STT

Tn khoa hc

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

22

CCTT

0,556

0,029

3,97

0,414 0,060

1,43

0,454 0,096

23

Wrightia dubia Spreng.

0,512

0,031

11,6

0,421 0,029

0,435 0,056

0,229

24

Mosla dianthera Maxim.

0,556

0,029

3,97

0,421 0,015

0,434 0,08

0,458

25

Cardiospermum halicacabum L.

0,563

0,016

2,76

0,393 0,023

6,43

0,453 0,083

26

Stemonurus coriaceus Miers

0,558

0,014

3,62

0,390 0,024

7,14

0,444 0,074

0,529

0,041

3,86

0,462 0,033

0,468 0,088

27

Dracaena gracilis Wall. ex

Hook.f.

28

Clerodendrum cyrtophyllum Tucz

0,591

0.036

0,393 0,063

7,14

0,468 0,058

29

Uncaria cordata Lour. Merr.

0,612

0,002

0,411 0,031

2,14

0,492 0,079

30

Smilax poilanei Gagnep.

0,503 0,052*

13,1

0,404 0,022

3,81

0,448 0,071

31

Illigera parviflora Dunn

0,608

0,031

0,411 0,025

2,14

0,464 0,074

32

Lygodium scandens

0,589

0,086*

0,417 0,027

0,714

0,465 0,067

33

Melastoma sanguineum Sims

0,549

0,058*

5,18

0,450 0,031

0,425 0,071

2,52

48

% c ch XO
STT

34
35

Tn khoa hc

Smilax corbularia Kunth

Streptocaulon juventas Lour.
Merr.

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

0,458

0,168

20,9

0,403 0,274

4,04

0,436 0,076

0,521

0,038

10,0

0,411 0,034

2,14

0,463 0,068

36

Ardisia gigantifolia Stapf

0,564

0,020

2,60

0,440 0,032

0,424 0,057

2,75

37

Pseuderanthemum bracteatum

0,546

0,002

5,70

0,407 0,030

3,09

0,455 0,075

0,517

0,026

10,7

0,444 0,019

0,452 0,084

38

Mussaenda cambodiana Pierre ex

Pitard

39

Hedyotis hedyotidea DC. Merr.

0,559

0,038

3,45

0,442 0,036

0,454 0,064

40

Psychotria rubra Poir.

0,605

0,006

0,468 0,038

0,48 0,068

41

Mussaenda densiflora Li

0,602

0,036*

0,467 0,021

0,465 0,074

42

Smilax perfoliata Lour.

0,476

0,002*

19,3

0,383 0,018

8,81

0,46 0,093

43

Premna fulva Craib

0,557

0,012

3,79

0,442 0,022

0,47 0,059

44

Selaginella uncinata Desv.Spring

0,582

0,038

0,353 0,013

15,9

0,465 0,082

49

% c ch XO
STT

45

Tn khoa hc

Hedyotis ovata Thwaites ex

Hook.f.

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

0,503

0,020

13,1

0,410 0,014

2,38

0,435 0,088

0,229

46

Dichroa febrifuga Lour.

0,548

0,036

5,35

0,421 0,017

0,451 0,071

47

Leptonichia acuminatus Mast.

0,475

0,101

17,9

0,390 0,021

7,14

0,433 0,073

0,688

48

Melastoma saigonense Merr.

0,413

0,155

28,7

0,416 0,030

0,952

0,436 0,076

49

Dianella ensifolia (L.) DC.

0,506

0,037

12,6

0,401 0,034

4,52

0,416 0,084

4,58

50

Smilax bracteata Presl

0,530

0,015

8,46

0,403 0,011

4,04

0,407 0,064

6,65

51

Anoectochilus roxburghii (Wall.)

0,492

0,027

15,0

0,371 0,027*

11,6

0,431 0,089

1,14

0,517

0,069

10,7

0,431 0,019

0,424 0,052

2,76

52

Hedyotis capitellata var. mollis

T.N.Ninh

53

Cancora petelotii

0,519

0,021

10,3

0,406 0,308

3,33

0,452 0,071

54

Dipterocarpus sp.

0,528

0,009

8,80

0,421 0,017

0,449 0,057

55

Wrightia laevis Hook.f.

0,466

0,006

19,5

0,406 0,022

3,59

0,451 0,066

56

CCTT

0,543

0,035

6,21

0,375 0,028*

10,7

0,441 0,084

50

% c ch XO
STT

Tn khoa hc

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

57

Rhynchodia pauciflora Pitard

0,548

0,082

5,35

0,440 0,013

0,459 0,072

58

Leea aequata L.

0,497

0,084

14,2

0,447 0,003

0,447 0,067

59

Cynoglossum zeylanicum

0,519

0,036

10,4

0.427 0,041

0,446 0,077

60

Chinacanthus nutans

0,466

0,176

19,5

0,472 0,022

0,464 0,064

61

Prunella vulgaris L.

0,509

0.082

12,1

0,449 0,019

0,45 0,063

62

Parashorea chinensis Wang Hsie.

0,509

0,055

12,1

0,431 0,030

0,461 0,077

63

Alangium kurzii Craib.

0,550

0,024

5,00

0,421 0,034

0,456 0,081

64

Piper bavinum C.DC.

0,500

0,012

13,6

0,419 0,020

0,23

0,439 0,064

65

Isotoma longiflora

0,567 0,057

2,07

0,381 0,126

9,28

0,469 0,074

66

Antidesma montanum Bl.

0,532

0,073

8,12

0,453 0,030

0,43 0,086

1,37

67

Viburnum lutescens Bl.

0,481

0,034

16,9

0,437 0,018

0,446 0,092

68

Hydnocarpus kurzii Warb.

0,547

0,071

5,52

0,404 0,037

3,81

0,462 0,071

69

Solanum torvum Swartz.

0,508

0,153

12,3

0,434 0,020

0,436 0,064

51

% c ch XO
STT

70

Tn khoa hc

Sambucus javanica Reinw. Ex

Blume

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

0,535

0,069

7,60

0,488 0,060

0,453 0,058

71

Crotalaria assamica Benth

0,593

0,055

0,439 0,035

0,454 0,068

72

Desmodium sequax Wall.

0,539

0,025

6,91

0,439 0,038

0,423 0,078

2,98

73

Afzelia xylocarpa Craib.

0,526

0,053

9,15

0,449 0,031

0,465 0,077

74

Callicarpa heterotricha Merr.

0,585

0,17

0,458 0,029

0,481 0,25

75

Miliusa balansae Fin. & Gagnep.

0,517

0,039

10,7

0,394 0,013

6,19

0,423 0,061

2,98

76

Knema globularia Warb.

0,554

0,081

4,31

0,372 0,010*

11,4

0,43 0,063

1,38

77

CCTT

0,557

0,025

3,79

0,408 0,019

2,85

0,439 0,073

78

Helicteres hirsuta Lour.

0,568

0,008

1.90

0,458 0,017

0,443 0,074

79

Hypericum uralum Thumb.

0,534

0,020

7,77

0,406 0,015

3,33

0,461 0,076

80

Callicarpa rubella

0,558

0,060

3,63

0,426 0,024

0,44 0,073

81

Derris pachyloba

0,529

0,037

8,63

0,405 0,028

3,57

0,456 0,074

52

% c ch XO
STT

Tn khoa hc

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

82

Cornus oligophlebia Merr.

0,547

0,012

5,52

0,453 0,053

0,438 0,065

83

Sterculia lanceolata Cav.

0,557

0,041

3,79

0,394 0,014

6,19

0,444 0,079

84

Uncaria scandens (Smith) Hutch.

0,528

0,038

8,80

0,445 0,022

0,474 0,083

85

Sabia parviflora Wall.ex Rox

0,524

0,030

9,49

0,462 0,028

0,455 0,071

86

Piper sp

0,636

0,024

0,562 0,026*

0,518 0,061

87

Itoa orientalis

0,603

0,035

0,469 0,009

0,5 0,071

88

Sambucus javanica Reinw.

0,555

0,055

6,92

0,471 0,028

0,46 0,051

89

Toddalia asiatica (L.) Lamk.

0,575

0,019

0,691

0,450 0,025

0,479 0,074

0,547

0,029

5,52

0,437 0,023

0,461 0,077

0,521

0,025

10,0

0,389 0,016

7,38

0,434 0,058

0,458

0,567

0,038

2,07

0,433 0,045

0,451 0,053

0,504

0,031*

12,9

0,361 0,058*

14,04

0,429 0,078

1,60

90
91
92
93

Uncaria macrophylla Wall. ex

Roxb.
Oroxylum indicum
Crotalaria assamica
Benth
Kibatalia macrophylla Woodson

53

% c ch XO
STT

94
95
96
97
98

Tn khoa hc

Epimedium macranthun Mooren

et Decne.
Rhabdosia sp.
Hypericum patulum Ham. Ex.
D.Don
Mallotus apelta Muell.-Arg.
Microdesmis casearifolia Planch.
ex Hook

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

0,542

0,027

6,39

0,422 0,034

0,44 0,068

0,492

0,047

15,0

0,404 0,023

3,81

0,424 0,061

2,75

0,441

0,099

23,8

0,347 0,031*

17,4

0,39 0,055

10,5

0,555

0,049

4,14

0,356 0,031*

15,2

0,37 0,066

15,1

0,494

0,038

14,7

0,382 0,022

9,04

0,418 0,086

4,13

99

Eleaocarpus nitidus Jack

0,525

0,089

9,32

0,404 0,034

3,81

0,441 0,057

100

Zanthoxylum avicennae DC.

0,482

0,089

16,75

0,407 0,022

3,09

0,42 0,071

3,67

101

Sapium sebiferum Roxb.

0,480

0,014

17,1

0,371 0,007*

11,6

0,388 0,061

11,0

102

Costus speciosus (Koenig) Sm.

0,510

0,031

11,9

0,408 0,017

2,86

0,444 0,07

103

Ficus heterophyllus L

0,541

0,036*

11,2

0,396 0,038

5,70

0,399 0,121

8,48

104

Cratoxylum pruniflorum Kurz

0,534

0,019

7,77

0,373 0,020

11,2

0,422 0,048

3,21

54

% c ch XO
STT

Tn khoa hc

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

105

Ficus nervosa

0,554

0,021

4,31

0,421 0,043

0,463 0,054

106

Knema elegans Warb.

0,557

0,013

3,79

0,442 0,049

0,431 0,064

1,15

107

Leea rubra Blunne

0,496

0,039

14,3

0,389 0,029

7,38

0,451 0,064

108

Antidesma ambigumPax & Hoffm

0,354

0,043*

38,8

0,326 0,046*

22,4

0,389 0,06

10,7

109

Osmanthus pedulculatus Gagnep.

0,539

0,032

6,90

0,393 0,032

6,43

0,434 0,064

110

sp1

0,555

0,065

4,14

0,388 0,017

7,61

0,425 0,054

2,52

0,472

0,051*

18,5

0,358 0,040*

14,7

0,429 0,073

1,60

0,499

0,061

13,8

0,395 0,030

5,95

0,451 0,08

0,522

0,030

9,84

0,394 0,053

6,19

0,416 0,05

4,58

0,548

0,014

5,35

0,428 0,015

0,457 0,07

0,478

0,017

17,4

0,368 0,019*

12,4

0,438 0,061

111

112
113
114
115

Caryodaphnosis tonkinensis
Airy-Shaw
Microdesmis casearifolia Planch.
ex Hook
Antidesma ghaesembilla Gaertn
Choerospondias axillaris Burtt.
& Hill
Aidia oxyodontaYamazaki

55

% c ch XO
STT

116

Tn khoa hc

Homalomena occulta (Lour)

schott

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

0,505

0,043

12,8

0,384 0,017

8,57

0,433 0,047

0,688

117

Cinnamomum camphora

0,562

0,008

2,93

0,409 0,017

2,62

0,436 0,072

118

Smilax riparia A.DC.

0,477

0,081

17,6

0,385 0,031

8,33

0,426 0,071

2,29

0,509

0,028

12,1

0,445 0,023

0,455 0,063

0,561

0,021

3,11

0,449 0,043

0,461 0,06

0,503

0,008

14,1

0,422 0,029

0,48 0,064

119
120
121

Pseuderanthemum palatiferum
(Nees) Radlk
Morinda officinalis How
Centipeda minima (L) A.Br.et
Aschers

122

Balanophora sp

0,557

0,055

13,1

0,382 0,017

9,05

0,451 0,055

123

Coptis teeta wall

0,487

0,031

15,9

0,391 0,032

6,90

0,447 0,068

124

Balanophora sp

0,539

0,011*

6,91

0,417 0,036

0,714

0,444 0,059

125

Hex godajam Colebr. ex. Well.

0,532

0,017

8,12

0,406 0,045

3,33

0,425 0,052

2,52

126

Oximum basilicum L.

0,577

0,019*

0,34

0,455 0,026

0,455 0,064

56

% c ch XO
STT

Tn khoa hc

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

127

Hex godajam Colebr. ex. Well.

0,539

0,041*

6,91

0,390 0,052

7,14

0,464 0,074

128

Ehretia Acuminata

0,525

0,041

9,32

0,354 0,019

15,7

0,419 0,057

3,90

129

Musra barjoo sieb.

0,515

0,046

11,0

0,382 0,041

9,04

0,444 0,059

130

Tadehagi Triquetrum L

0,561

0,018

3,11

0,434 0,023

0,44 0,063

131

Ardisia silvestris

0.483

0,021

16,6

0,412 0,049

1,90

0,446 0,062

132

Bridelia balansae

0,647

0,052*

0,465 0,035

0,465 0,059

133

Houltuynia cordata Thumb

0,545

0,064*

5,87

0,419 0,016

0,238

0,476 0,072

134

Clausena excavata Burm.f.

0,564

0,035

2,59

0,391 0,024

6,90

0,466 0,083

135

Ixora spp.

0,536

0,015

7,42

0,434 0,028

0,468 0,072

136

Stephania dielsiana Y.C.Wu

0,537

0,026

7,25

0,429 0,024

0,449 0,074

137

Salacia cochinchinensis

0,423

0,039*

26,9

0,420 0,064

0,425 0,041

2,52

138

Ammania baccifera L.

0,525

0,002

9,32

0,416 0,065

0,952

0,443 0,046

139

Cassytha filiformis L.

0,532

0,031

8,12

0,398 0,018

5,24

0,408 0,06

6,42

57

% c ch XO
STT

Tn khoa hc

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

140

Ficus heterophyllus L

0,540

0,007

6,73

0,418 0,018

0,476

0,443 0,048

141

Passiflora foetida L.

0,550

0,032

5,00

0,433 0,023

0,425 0,048

2,52

142

Eurya ciliata Merr L

0,530

0,031

8,46

0,376 0,045

10,5

0,387 0,051

11,23

143

Ventilago leiocarpa Benth.

0,512

0,073

11,57

0,353 0,015

15,9

0,383 0,075

12,15

144

Abutilon indicum L sweet

0,571

0,136

1,38

0,429 0,023

0,424 0,053

2,75

0,539

0,046

6,91

0,384 0,031

8,57

0,437 0,048

145

Flueggea virosa Roxbex willd

Voigt

146

CCTT

0,552

0,051*

4,66

0,401 0,018

4,50

0,423 0,071

2,98

147

Glycosmis pentaphylla Correa

0,523

0,037

9,67

0,392 0,036

6,66

0,389 0,064

10,8

0,539

0,024

6,91

0,407 0,024

3,09

0,438 0,075

148

Strobilanthes cystolithigera
Lindau

149

Homonoia riparia Lour.

0,575

0,023

0,691

0,398 0,006

5,24

0,449 0,044

150

Cyclea fansipanensis Gagnep

0,574

0,006*

0,863

0,429 0,038

0,359 0,176

17,6

58

% c ch XO
STT

Tn khoa hc

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

151

Costus speciosus Koenig Sm.

0,545

0,005

5,87

0,405 0,013

3,57

0,462 0,086

152

Curculigo orchioides Gaertr

0,515

0,027

11,0

0,413 0,033

4,86

0,454 0,047

153

Kaempferia galanga. L

0,497

0,063

14,2

0,425 0,041

1,67

0,413 0,059

5,27

154

Ehretia acuminata

0,518

0,048

10,5

0,417 0,038

0,714

0,41 0,1

5,96

0,528

0,015

8,80

0,382 0,026

9,04

0,431 0,092

1,14

155

Desmodium rodgeri Schindl.

Ohashi

156

Rubus leucanthus Hance

0,527

0,014

10,0

0,390 0,048

7,14

0,427 0,063

2,06

157

Boehmeria nivea L. Gaud.

0,584

0,009*

0,443 0,013

0,437 0,058

158

Scheffena octophylla Lour

0,564

0,018

2,59

0,445 0,042

0,448 0,071

159

Sarcandra glabra Thunb. Nakai

0,540

0,028

6,73

0,414 0,044

1,43

0,414 0,087

5,04

160

Rubus cochinchinensis Tratt.

0,557

0,021

3,79

0,429 0,024

0,455 0,051

161

Pothos scandens L.

0,556

0,002

5,04

0,382 0,098

9,04

0,437 0,097

162

Ehretia asperrula Zoll & Mor

0,485

0,018

16,2

0,413 0,059

1,67

0,453 0,068

163

Stephania longa Lour.

0,564

0,018

2,59

0,382 0,033

9,04

0,451 0,052

59

% c ch XO
STT

164
165

Tn khoa hc

Rourea minor Gaertn Leenh.

Kadsura heteroclita Roxb.
Craib

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

0,542

0,022

6,39

0,411 0,062

2,14

0,435 0,075

0,229

0,455

0,009

21,4

0,388 0,064

7,61

0,443 0,033

166

Limacia scandes Lour.

0,569

0,012

1,72

0,417 0,042

0,714

0,47 0,07

167

Ardisia silvestris Pitard

0,542

0,006

6,39

0,404 0,040

3,81

0,471 0,054

168

Rauvolfia verticilata Lour. Bail

0,555

0,017

4,14

0,430 0,043

0,454 0,065

0,524

0,005

9,49

0,376 0,052

10,47

0,457 0,061

0,555

0,019

4,14

0,415 0,087

1,19

0,449 0,057

169

170

Mussaenda cambodiana Pierre ex

Pitard
Homalomena occulta Lour
schott

171

Mucuna pruriens L. DC.

0,543

0,016

6,21

0,450 0.029

0,411 0,03

5,73

172

Solanum erianthum D.Don

0,555

0,011

4,14

0,398 0,041

5,23

0,456 0,059

173

Asarum balansae Franch.

0,440

0,006*

24,0

0,400 0,035

4,76

0,451 0,059

60

% c ch XO
STT

174
175
176
177

Tn khoa hc

Erythropalum scandens Blume

Curculigo latifolia Dryand. ex
Ait.
Ophiopogon reptans Hook
Anisomeles indica
L Kuntze

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

0,494

0,036

14,6

0,419 0,043

0,238

0,428 0,067

1,83

0,509

0,038

12,1

0,455 0,027

0,453 0,069

0,514

0,011

11,2

0,455 0,056

0,434 0,058

0,458

0,533

0,039

7,94

0,467 0,033

0,457 0,062

178

Ardisia gracillima

0,565

0,052

2,42

0,455 0,027

0,466 0,075

179

Zanthoxylum avicennae DC.

0,575

0,045

0,691

0,455 0,056

0,473 0,071

0,571

0,047

1,38

0,467 0,033

0,45 0,054

180

Amalocalyse miceolobus
Pierre ex Spire

181

Paramygnya petebotii Guill.

0,563

0,032

2,76

0,473 0,026

0,494 0,085

182

Chloranthus japonicusSieb

0,539

0,013

6,91

0,459 0,022

0,456 0,052

183

Equisetum diffusum D.Don

0,547

0,025

5,52

0,442 0,038

0,429 0,066

1,60

184

Ilex glomerata King

0,547

0,036

5,52

0,439 0,034

0,452 0,09

61

% c ch XO
STT

185
186
187

Tn khoa hc

Pseuderanthemum palatiferum
Nees Radlk.
Iodes cirrhosa Turcz.
Strobilanthes
mucronatoproductus Lindau

Nng 100 g/ml

Nng 50 g/ml

Nng 10 g/ml

OD

I%

OD

I%

OD

I%

0,043

11,6

0,424 0,054

0,434 0,052

0,458

0,482 0,046

16,7

0,389 0,038

7,38

0,426 0,086

2,29

0,515

0,025

11,0

0,403 0,025

4,04

0,407 0,061

6,65

0,512

188

Clerodendrum paniculatum L.

0,511

0,056

11,7

0,389 0,046

7,38

0,42 0,063

3,66

189

Belamcanda chinensis L. DC.

0,423

0,167

26,9

0,348 0,036*

17,1

0,377 0,04*

13,5

190

Lygodium flexuosum L. Sw.

0,204

0,032*

64,8

0,254 0,031*

39,5

191

Pandanus tinctorius

0,576

0,061

0,52

0,435 0,027

0,429 0,079

1,60

192

Euodia lepta

0,525

0,041

9,32

0,434 0,045

0,426 0,064

2,29

193

Tabernaemontana buffalina

0,517

0,028

10,7

0,380 0,042

9,52

0,42 0,035

3,67

194

Euphorbia hirta L.

0,548

0,034

5,35

0,433 0,021

0,444 0,057

195

Carallia brachiata

0,483

0,112

16,6

0,423 0,031

0,454 0,069

0,333
0,108*

23,6

62

% c ch XO
STT

Tn khoa hc

Nng 100 g/ml

Nng 100 g/ml

Nng 100 g/ml

OD

I%

OD

I%

OD

I%

196

Helicteres angustifolia

0,528

0,052

8,88

0,470 0,016

0,41 0,073

5,96

197

Tinospora sinensis

0,563

0,040

2,76

0,449 0,043

0,44 0,057

198

Genianthus laurifolius

0,531

0,038

8,29

0,440 0,036

0,457 0,07

199

Strobilanthes apricus

0,521

0,016

10,0

0,428 0,028

0,419 0,054

3,89

200

Alseodaphne sp.

0,527

0,042

8,98

0,456 0,046

0,451 0,049

201

Acronychia pedunculata

0,498

0,012

14,0

0,422 0,037

0,424 0,054

2,75

202

Actinodaphne pilosa Merr.

0,537

0,059

7,25

0,425 0,019

0,417 0,074

4,35

Mu chng

0,579

0,014

0,420

0,018

*, p < 0,05 khi so snh vi mu chng.

0,436

0,07

63

3.1.3. Xc nh IC50 ca cc dc liu tim nng c ch XO

5 mu th t 5 dc liu c tc dng c ch XO mnh nht ti nng 100
g/ml l : Antidesma ambigum, Caryodaphnosis tonkinensis, Asarum balansae
Salacia cochinchinensis v Lygodium flexuosum c thit lp dy nng kho
st v tin hnh xc nh IC50.
Allopurinol - mt thuc c ch mnh XO, c tc dng lm h acid uric huyt
thanh trong lm sng c s dng lm cht i chiu trong th nghim.
Kt qu c trnh by bng 3.5.
Bng 3.3. IC50 ca cc dc liu tim nng c ch XO
Mu th

c ch hot XO
IC50 ( g/ml)

108. Antidesma ambigum

105,3 (53,4 - 180,8)

111. Microdesmis casearifolia

311,8 (239,1 - 399,3)

137. Salacia cochinchinensis

295,4 (191,9 - 396,1)

173. Asarum balansae

508,9 (420,7 - 703,3)

190. Lygodium flexuosum

83,6 (47,2 - 129,6)

Allopurinol

0,456 (0,416 0,497)

Nhn xt:
Lygodium flexuosum l cy c kh nng c ch XO mnh nht trong s 5 cy
thuc chn, c xc nh IC50 = 83,6 (47,2 - 129,6) g/ml.

3.2. Bn lun
3.2.1. V thng tin ca cc cy thuc a vo nghin cu
Trong 187 cy thuc c a vo nghin cu, 111 cy thuc c s dng
ph bin trong dn gian Vit Nam. Trn th gii, cng c nhiu nghin cu c th
v tc dng, thnh phn ho hc v cc hot tnh sinh hc ca chng. Th d cc cy
thuc nh thin nin kin (Homalomera occulta), dm dng hoc (Epimedium
macranthun), long no (Cinnamomum camphora) c s dng nhiu trong dn
gian cha cc bnh vim khp, au gn ct ...[10] v trn th gii cc cy ny

64

cng cng c nghin cu nhiu v tc dng chng vim, chng oxy ho. Bn
cnh , 76 cy thuc nh v hng giai (Stemonurus coriaceus Miers), bng bong
(Lygodium scandens), mua lng (Melastoma saigonense), cm lng (Eleaocarpus
nitidus) ... mi ch c s dng trong dn gian Vit Nam, trn th gii cha c cc
nghin cu v tc dng ca cc cy thuc ny.
Mt s cy thuc nh thng sn (Dichroa febrifuga Lour), bm bc
campuchia (Mussaenda cambodiana), mua b (Melastoma sanguineum), ... cha c
cc nghin cu v tc dng ca b phn dng nh trong cc mu c sng lc.
Ngoi ra, mt s mu cng loi nhng c thu hi cc vng khc nhau (ph lc
1) v mt s mu cng loi nhng khc b phn dng (ph lc 2) c a vo
sng lc so snh kh nng c ch XO gia cc mu , t tm ra mu c tc
dng c ch XO tt hn.
3.2.2. V kt qu sng lc tc dng c ch XO in vitro trn a Costar 96 ging
Trong giai on sng lc, tm ra cc cy thuc c kh nng iu tr gt,
nghin cu sng lc in vitro nh gi tc dng c ch XO ca cc cy thuc thng
c ngh n u tin v nghin cu ny hon ton c th trin khai c v n
kh hp l vi iu kin nghin cu trong nc. Tc gi Nguyn Th Thanh Mai v
cng s thc hin cng trnh nghin cu ca mnh vi cc dc liu c thu hi
cc tnh pha Nam [86]. Trong khi , khu vc min Trung v min Bc cha c
nghin cu sng lc no. V vy, ti "Sng lc cc cy thuc c tim nng iu
tr bnh gt thng qua c ch XO in vitro" l cng trnh nghin cu u tin c
tin hnh vi cc cy thuc c thu hi ti cc tnh min Trung v min Bc.
sng lc tc dng c ch XO ca dc liu, kho lun tin hnh th
nghim in vitro trn a 96 ging theo phng php ca Noro [93] v c sa i
ph hp vi iu kin phng th nghim. i vi cc dch chit dc liu, cc tc
gi thng thc hin nghin cu in vitro vi ba nng ca mu th l 100 g/ml,
50 g/ml v 10 g/ml [121], [86]. sng lc 202 mu dc liu trong nghin cu
ny, kho lun cng p dng 3 mc nng 100 g/ml, 50 g/ml v 10 g/ml cho
tt c mu th nh gi nh hng ca dch chit ln hot XO in vitro. Qu

65

trnh sng lc c lm trn a 96 ging nn c th tin hnh vi nhiu nng ,

lp li th nghim vi nhiu a trong khong thi gian ngn v c th tin hnh
php o nhanh chng, lin tip, t ng. Tuy nhin, a Costar 96 ging s dng
lm thc nghim thng c dng o vi bc sng ln hn 340nm. Khi p
dng trong nghin cu ny, bc sng s dng l 290nm, do sai s mc phi kh
ln.
Trong 202 mu dc liu c sng lc, ti nng 100 g/ml c 20 mu
c tc dng c ch XO, 13 mu c tc dng c ch XO nng 50 g/ml v c 3
mu c tc dng c XO nng 10 g/ml. Bn cnh , cc dc liu c tc
dng c ch XO khng tp trung m phn b ri rc cc h khc nhau. Nh vy,
so vi cc nghin cu sng lc khc, t l cc cy thuc c tc dng c ch XO
khng cao v cc nghin cu trc tp trung sng lc cc dc liu c s dng
theo kinh nghim dn gian hoc theo y hc c truyn vi tc dng chng vim,
chng oxy ho. Trong khi , cc dc liu c sng lc trong ti ny l nhng
cy thuc thuc d n "Bo tn ngun cy thuc c truyn", trong dn gian v y
hc c truyn khng ch c cc cy thuc c dng vi tc dng chng vim,
chng oxy ho m cn c nhiu cy thuc c dng vi cc tc dng khc nh
chng ung th, an thn, h huyt p ...
3.2.3. V kh nng c ch XO ca cy thuc tim nng
Kho lun tin hnh xc nh IC50 trn 5 mu th c kh nng c ch XO
mnh nht nng 100 g/ml l: Antidesma ambigum, Microdesmis casearifolia,
Salacia cochinchinensis, Asarum balansae v Lygodium flexuosum.
Antidesma ambigum v Asarum balansae hin cha c bo co v thnh
phn ho hc, hot tnh sinh hc. Tuy nhin trong chi Antidesma c cy Antidesma
madagascariense v Antidesma thwaitesianum c nghin cc c kh nng
chng oxy ho [78], [101] v trong chi Asarum c cy Asarum sieboldii c
nghin cu v tc dng chng vim vi nhiu hot cht c phn lp [102].
Vi IC50 > 500 g/ml, Asarum balansae l cy c tc dng c ch XO yu
nht trong 5 cy, yu hn rt nhiu so vi allopurinol. Antidesma ambigum l cy

66

c kh nng c ch XO mnh th 2 vi IC50 = 105,3 g/ml (53,4 - 180,8). Mc d

cha c bo co khoa hc no v Antidesma ambigum nhng cc hp cht
polyphenolic c bo co cng hot ng chng oxy ho trong chi Antidesma
l cy Antidesma thwaitesianum [101]. Nh vy hot ng c ch XO ca
Antidesma ambigum cng c th do s hin din ca cc thnh phn phenolic tng
t nh cc cy khc trong chi.
Microdesmis casearifolia cha thy c bo co v thnh phn ho hc cng
nh cc tc dng ca n. Cy Salacia cochinchinensis mi ch c cc bo co v tc
dng h ng huyt [8].
Dch chit methanol ca Lygodium flexuosum c kh nng c ch XO mnh
nht trong 5 dch chit t 5 cy c tim nng iu tr gt c xc nh IC50 vi
IC50 = 83,6 g/ml, tuy nhin vn thp hn nhiu so vi allopurinol vi IC50 = 0,456
g/ml c th v dch chit c dng trong nghin cu l dch chit ca c cy,
khng phi cht tinh khit nn kh nng c ch km hn nhiu so vi allopurinol.
Lygodium flexuosum l cy thuc kh ni bt vi nhiu nghin cu v tc dng
chng vim, chng oxy ho [33]. Trong mt nghin cu v cc thuc s dng trong
dn gian ca n , r ca cy Lygodium flexuosum c dng iu tr thp
khp v cc bnh vim nhim [108]. Kh nng chng oxy ho ca cy thuc ny
c nghin cu rng ri nht vi cc hp cht phenolic c phn lp. Bn
cnh cc phn on methanol cng cho thy s hin din ca quercetin-1
flavonoid in hnh c ch XO [56]. Nh vy, kh nng c ch XO ca Lygodium
flexuosum c th do cc phenolic v quercetin c mt trong thnh phn dch chit
methanol ca cy.
Qu trnh sng lc cc dc liu c kh nng c ch XO pht hin ra
Lygodium flexuosum - cy thuc c tim nng nht. cng l c s nghin cu
su hn v tc dng dc l cng nh cc thnh phn ho hc ca Lygodium
flexuosum, t c th ng dng trong iu tr.

67

KT LUN
1. Kt lun
Trong khun kh ti: Sng lc cc cy thuc c tim nng iu tr bnh
gt thng qua c ch XO in vitro, kho lun thu c nhng kt qu sau:
1. thu thp c c bn thng tin khoa hc ca 202 mu cy thuc, thuc
74 h a vo nghin cu.
2. Trong s 202 dch chit methanol t 187 loi:
- Ti nng 100g/ml c 20 mu c tc dng c ch XO, ch c 1 mu c tc
dng c ch > 50%.
- Ti nng 50g/ml c 13 mu c tc dng c ch XO, khng c mu no
c tc dng c ch > 50%.
- Ti nng 10g/ml c 3 mu c tc dng c ch XO, khng c mu no c
tc dng c ch >50%
3. xc nh c IC50 ca 5 dc liu tim nng nht. Dch chit
methanol ca Lygodium flexuosum c IC50 = 83,6 (47,2 - 129,6) g/ml th hin kh
nng c ch XO mnh nht trong cc mu nghin cu.

2. xut
Vi nghin cu in vitro trn a 96 ging, kho lun mi ch c th sng lc
v xc nh IC50 ca cc cy thuc c tim nng c ch XO. Do cn c nhng
nghin cu dc l thc nghim tip theo khng nh tc dng ca cc cy thuc
ny. Kho lun xin a ra hai xut trin khai nghin cu tip nh sau:
-

M rng thm i tng dc liu a vo sng lc.

nh gi tc dng h acid uric huyt thanh ca cy Lygodium flexuosum

in vivo nh hng cho nghin cu su tip theo v dc liu ny.

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PH LC
Ph lc 1. Cc mu cy cng tn loi

STT

Mu

Cy thuc

Mu 8 v 157

Gai (Bohmeria nivea L. Gaud.)

Mu 14 v 26

V hung giai (Stemonurus coriaceus Miers)

Mu 71 v 92

Lc lc l i (Crotalaria assamica Benth)

Mu 98 v 112

Chn (Microdesmis casearifolia)

Mu 100 v 179

Mung trung (Zanthoxylum avicennae)

Mu 102 v 151

Ma d (Costus speciosus Koenig Sm.)

Mu 116 v 170

Thin nin kin (Homalomena occulta )

Mu 119 v 185

Con kh (Pseuderanthemum palatiferum)

Mu 128 v 154

Cm rng (Ehretia acuminata)

10

Mu 131 v 167

L khi (Ardisia silvestris)

Ph lc 2. Cc mu cy cng loi nhng khc b phn dng

STT

Mu

Cy thuc

Mu 38 v 169

Mu 70 v 88

Cm chy (Sambucus javanica Reinw.)

Mu 103 v 140

V ch (Ficus heterophyllus L)

Mu 122 v 124

C gi t (Balanophora sp.)

Mu 125 v 127

Rt (Hex godajam Colebr. ex. Well.)

Bm bc campuchia (Mussaenda cambodiana

Pierre ex Pitard)