The 4th Annual PCR Technology Workshop:

HLA Typing by Molecular Methods

The Human Leucocyte Antigen (HLA) typing is the most important histocompatibility test with
particular relevance to organ/ tissue transplants. The clinical applications of HLA typing include 1)
typing for renal transplantation, 2) typing for haematopoeitic stem cell transplant, and 3) typing as an
aid to HLA-associated diseases.
HLA typing has transited from serologic typing to DNA based typing in most histocompatibility testing laboratories.
This workshop focuses on DNA based typing methods including PCR-SSP, PCR-SSOP, PCR-RFLP
and Sequence-based typing (SBT). It is a hands-on intensive course that will enable participants
acquire skills for setting up and conducting HLA typing.
Course Content

Target Audience

Introduction to HLAs

Medical Laboratory Scientists

HLA Genes, alleles, proteins and antigens

Medical Doctors, Pathologists, Haematologists,

Immunologists

Structure-function of HLA molecules

Genetics of the Major Histocompatibility Complex
HLA Nomenclature/ HLA Dictionary
Serologic HLA typing
Molecular Methods for HLA typing
PCR-SSP/ PCR-SSOP methods
PCR-RFLP method
Sequence-Based typing method (SBT)
Practical sessions
Clinical Impact of Molecular typing
Establishing HLA laboratory

Academics, Research Fellows and Scientists

Pharmacists
PhD Students and Post-Doctorates
Venue: :

Safety Molecular Pathology Laboratory

Faculty of Health Sciences & Technology, University of Nigeria,

Enugu Campus, Enugu, Nigeria
Date : 10-14, September, 2012
for 20 participants only
Registration fee :: 60,000 Naira

Sourcing reagents for HLA typing

Accreditation, Certification of HLA lab
Proficiency testing
The Nigerian Bone Marrow Registry
The IMGT/HLA Database
HLA Laboratory software: the Orpheus, etc

QC and QA in HLA lab

All fees are payable to :

Account Name: Safety Molecular Path Lab Services Ltd
Bank: Zenith bank plc, Account Number: 1012967770
Please email your Payment Teller Number to e.nna@safetybiomedical.org
Further inquires: contact 07063415385 or 07034959316 or 08037435616
Or email: e.nna@safetybiomedical.org

Inauguration of the Nigerian Society for Histocompatibility and Immunogenetics (NSHI).

Workshop Co-ordinator: E. Nna, PhD (Cranfield University, UK). Dr Nna is the Head of Safety Molecular Pathology Laboratory in Nigeria,
which provides HLA typing, paternity testing, human identity and cancer diagnosis .

www.safetybiomedical.org