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    Noradrenalina Concentración (M) Contracción (G) Log C Ecto Emax-E

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    Christian Camacho
    The document contains data from experiments measuring the concentration-dependent contraction of arteries in response to noradrenaline and the relaxation of arteries and airways in response to carbacol and isoproterenol. The data is presented in tables and graphs showing the relationship between agonist concentration and tissue response, and the curves are fitted to linear, Hill equation and semilog models. Pharmacological parameters like EC50 and Emax are calculated from the curve fits.

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    Noradrenalina Concentración (M) Contracción (G) Log C Ecto Emax-E

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    Christian Camacho
    9 views21 pages
    The document contains data from experiments measuring the concentration-dependent contraction of arteries in response to noradrenaline and the relaxation of arteries and airways in response to carbacol and isoproterenol. The data is presented in tables and graphs showing the relationship between agonist concentration and tissue response, and the curves are fitted to linear, Hill equation and semilog models. Pharmacological parameters like EC50 and Emax are calculated from the curve fits.

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    The document contains data from experiments measuring the concentration-dependent contraction of arteries in response to noradrenaline and the relaxation of arteries and airways in response to carbacol and isoproterenol. The data is presented in tables and graphs showing the relationship between agonist concentration and tissue response, and the curves are fitted to linear, Hill equation and semilog models. Pharmacological parameters like EC50 and Emax are calculated from the curve fits.

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    9 views21 pages

    Noradrenalina Concentración (M) Contracción (G) Log C Ecto Emax-E

    Uploaded by

    Christian Camacho
    The document contains data from experiments measuring the concentration-dependent contraction of arteries in response to noradrenaline and the relaxation of arteries and airways in response to carbacol and isoproterenol. The data is presented in tables and graphs showing the relationship between agonist concentration and tissue response, and the curves are fitted to linear, Hill equation and semilog models. Pharmacological parameters like EC50 and Emax are calculated from the curve fits.

    Copyright:

    © All Rights Reserved
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    of 21

    NORADRENALINA

    Concentracin (M)

    Contraccin (g)

    Log C

    %efecto

    Emax-E

    1.00E-09
    3.16E-09
    1.00E-08
    3.16E-08
    1.00E-07
    3.16E-07
    1.00E-06
    3.16E-06

    0.0497
    0.219
    0.4681
    0.7065
    1.0144
    1.2025
    1.3806
    1.47

    -9.00
    -8.50
    -8.00
    -7.50
    -7.00
    -6.50
    -6.00
    -5.50

    3.38
    14.90
    31.84
    48.06
    69.01
    81.80
    93.92
    100.00

    96.62
    85.10
    68.16
    51.94
    30.99
    18.20
    6.08
    0.00

    Contraccin aorta (lineal)

    100.00
    90.00
    80.00

    % Efecto

    70.00
    60.00
    50.00
    40.00
    30.00
    20.00
    10.00
    0.00
    0.00E+00

    5.00E-07

    1.00E-06

    1.50E-06

    2.00E-06

    2.50E-06

    3.00E-06

    3.50E-06

    Concentracin noradrenalina(M)

    Contraccin aorta (Hill)


    1.50E+00
    1.00E+00

    Log[E/(Emax -E)]

    f(x) = 0.8165360204x + 6.0687150383


    R = 0.9842807748

    5.00E-01
    -9.50

    -9.00

    -8.50

    -8.00

    -7.50

    -7.00

    -6.50

    0.00E+00
    -6.00
    -5.50
    -5.00E-01
    -1.00E+00
    -1.50E+00
    -2.00E+00

    LogC noradrenalina(M)

    Lo

    -1.50E+00
    -2.00E+00

    LogC noradrenalina(M)

    E/(Emax-E)

    Log[E/(Emax-E)]

    CE50(slog)

    0.0349926072
    0.175059952
    0.4672122966
    0.9253438114
    2.2265144864
    4.4953271028
    15.4429530201
    -----------

    -1.46E+00
    -7.57E-01
    -3.30E-01
    -3.37E-02
    3.48E-01
    6.53E-01
    1.19E+00
    ----------

    3.70E-08

    Contraccin aorta (semilog)


    100.00

    f(x) = 29.5786512872x + 269.8137952472


    R = 0.9866429522

    90.00

    % Efecto

    80.00
    70.00
    60.00
    50.00
    40.00
    30.00
    20.00
    10.00

    00E-06

    3.50E-06
    -9.50

    -9.00

    -8.50

    -8.00

    -7.50

    Log C noradrenalina(M)

    1.50E+00
    1.00E+00
    5.00E-01
    0.00E+00
    -6.00
    -5.50
    -5.00E-01
    -1.00E+00
    -1.50E+00
    -2.00E+00

    -7.00

    -6.50

    -6.00

    -5.50

    0.00
    -5.00

    -1.50E+00
    -2.00E+00

    -5.50

    100.00
    90.00
    80.00
    70.00
    60.00
    50.00
    40.00
    30.00
    20.00
    10.00
    0.00
    -5.00

    CARBACOL
    Concentracin Contraccin
    (M)
    (g)
    1.00E-09
    3.16E-09
    1.00E-08
    3.16E-08
    1.00E-07
    3.16E-07
    1.00E-06
    3.16E-06
    1.00E-05

    1.5888
    1.5426
    1.4502
    1.2177
    0.9558
    0.4064
    0.2586
    0.1939
    0.157

    Contraccion

    1.6072

    Log C

    %No Efecto

    %efecto

    Emax-E

    -9.00
    -8.50
    -8.00
    -7.50
    -7.00
    -6.50
    -6.00
    -5.50
    -5.00

    98.86
    95.98
    90.23
    75.77
    59.47
    25.29
    16.09
    12.06
    9.77

    1.14
    4.02
    9.77
    24.23
    40.53
    74.71
    83.91
    87.94
    90.23

    98.86
    95.98
    90.23
    75.77
    59.47
    25.29
    16.09
    12.06
    9.77

    Relajacin aorta (lineal)

    0.00
    10.00

    % Efecto

    20.00
    30.00
    40.00
    50.00
    60.00
    70.00
    80.00
    90.00
    100.00
    0.00E+00

    2.00E-06

    4.00E-06

    6.00E-06

    8.00E-06

    1.00E-05

    1.20E-05

    Concentracin carbacol(M)

    Relajacin aorta (Hill)

    Log[E/(Emax-E)]

    1.50E+00
    f(x) = 0.7553356098x + 5.0735214087
    R = 0.9666320841

    1.00E+00

    5.00E-01
    -9.50

    -9.00

    -8.50

    -8.00

    -7.50

    -7.00

    -6.50

    -6.00

    -5.50

    -5.00

    0.00E+00
    -4.50
    -5.00E-01

    -1.00E+0

    -1.50E+0

    -2.00E+0

    -2.50E+0

    Log C carbacol

    Lo

    -1.00E+0

    -1.50E+0

    -2.00E+0

    -2.50E+0

    Log C carbacol

    E-05

    .50

    E/(Emax-E)

    Log[E/(EmaxE)]

    0.0115810675
    0.0418773499
    0.1082609295
    0.3198653199
    0.6815233312
    2.9547244094
    5.215003867
    7.2888086643
    9.2369426752

    -1.94E+00
    -1.38E+00
    -9.66E-01
    -4.95E-01
    -1.67E-01
    4.71E-01
    7.17E-01
    8.63E-01
    9.66E-01

    CE50(Hill)
    1.92E-07

    Relajacin aorta (semilog)


    0.00

    f(x) = 26.8951001701x + 234.545919681


    R = 0.9380556596

    10.00

    % Efecto

    20.00
    30.00
    40.00
    50.00
    60.00
    70.00
    80.00
    90.00

    1.20E-05

    -9.50

    -9.00

    -8.50

    -8.00

    -7.50

    -7.00

    Log C carbacol

    1.50E+00
    1.00E+00
    5.00E-01
    -5.00

    0.00E+00
    -4.50
    -5.00E-01
    -1.00E+00
    -1.50E+00
    -2.00E+00
    -2.50E+00

    -6.50

    -6.00

    -5.50

    -5.00

    100.00
    -4.50

    -1.00E+00
    -1.50E+00
    -2.00E+00
    -2.50E+00

    0.00
    10.00
    20.00
    30.00
    40.00
    50.00
    60.00
    70.00
    80.00
    90.00
    -5.50

    -5.00

    100.00
    -4.50

    CARBACOL
    Concentracin (M)

    Contraccin (g)

    Log C

    %efecto

    Emax-E

    1.00E-06
    3.16E-06
    1.00E-05
    3.16E-05
    1.00E-04

    0.107
    0.316
    0.646
    0.875
    1.015

    -6.00
    -5.50
    -5.00
    -4.50
    -4.00

    10.54
    31.13
    63.65
    86.21
    100.00

    89.46
    68.87
    36.35
    13.79
    0.00

    100.00

    Contraccin trquea (lineal)

    90.00
    80.00

    % Efecto

    70.00
    60.00
    50.00
    40.00
    30.00
    20.00
    10.00
    -2.00E-05

    0.00
    1.69E-20

    2.00E-05

    4.00E-05

    6.00E-05

    8.00E-05

    1.00E-04

    Concentracin carbacol(M)

    Contraccin trquea (Hill)


    1.00E+00

    Log[E/(Emax -E)]

    f(x) = 1.1524946571x + 5.992175769


    R = 0.9998096509

    -6.20

    -6.00

    -5.80

    -5.60

    -5.40

    -5.20

    5.00E-01

    -5.00

    -4.80

    0.00E+00
    -4.60
    -4.40
    -5.00E-01
    -1.00E+00
    -1.50E+00

    LogC carbacol(M)

    Lo

    -1.00E+00
    -1.50E+00

    LogC carbacol(M)

    E/(Emax-E)

    Log[E/(Emax-E)]

    CE50(Hill)

    0.1178414097
    0.452074392
    1.7506775068
    6.25
    -----

    -9.29E-01
    -3.45E-01
    2.43E-01
    7.96E-01
    -----

    6.32E-06

    Contraccin trquea(semilog)

    f(x) = 46.7979361024x + 292.3009567863


    R = 0.983823542

    % Efecto

    E-05

    1.00E-04
    -6.50

    -6.00

    -5.50

    -5.00

    Log C carbacol(M)

    1.00E+00
    5.00E-01
    0.00E+00
    -4.60
    -4.40
    -5.00E-01
    -1.00E+00
    -1.50E+00

    -4.50

    -4.00

    0
    -3.50

    -1.00E+00
    -1.50E+00

    ilog)
    100.00

    7863

    90.00
    80.00
    70.00
    60.00
    50.00
    40.00
    30.00
    20.00
    10.00
    -4.00

    0.00
    -3.50

    ISOPROTERENOL
    Concentracin Contraccin
    (M)
    (g)
    1.00E-06
    3.16E-06
    1.00E-05
    3.16E-05
    1.00E-04

    0.987
    0.756
    0.564
    0.228
    0.095

    Contraccin (g)

    1.015

    Log C

    %No Efecto

    %efecto

    Emax-E

    -6.00
    -5.50
    -5.00
    -4.50
    -4.00

    97.24
    74.48
    55.57
    22.46
    9.36

    2.76
    25.52
    44.43
    77.54
    90.64

    97.24
    74.48
    55.57
    22.46
    9.36

    Relajacin trquea (lineal)

    0.00
    10.00

    % Efecto

    20.00
    30.00
    40.00
    50.00
    60.00
    70.00
    80.00
    90.00
    100.00
    0.00E+00

    2.00E-05

    4.00E-05

    6.00E-05

    8.00E-05

    1.00E-04

    1.20E-04

    Concentracin carbacol(M)

    Relajacin trquea (Hill)

    Log[E/(Emax-E)]

    1.50E+00

    1.00E+00

    f(x) = 1.2139032412x + 5.9525922251


    R = 0.9661840733

    -6.50

    -6.00

    -5.50

    -5.00

    5.00E-01

    -4.50

    -4.00

    0.00E+00
    -3.50

    -5.00E-01

    -1.00E+00

    -1.50E+00

    -2.00E+00

    Log C carbacol

    E/(Emax-E)

    Log[E/(EmaxE)]

    0.0283687943
    0.3425925926
    0.7996453901
    3.451754386
    9.6842105263

    -1.55E+00
    -4.65E-01
    -9.71E-02
    5.38E-01
    9.86E-01

    CE50(slog)
    1.10E-05

    Relajacin trquea (semilog)


    0.00
    f(x) = 45.5558095482x + 275.9620897246
    R = 0.9872277059

    10.00
    20.00

    % Efecto

    30.00
    40.00
    50.00
    60.00
    70.00
    80.00
    90.00

    E-04

    -6.50

    1.20E-04

    -6.00

    -5.50

    -5.00

    Log C carbacol

    1.50E+00
    1.00E+00
    5.00E-01

    -4.00

    0.00E+00
    -3.50
    -5.00E-01
    -1.00E+00
    -1.50E+00
    -2.00E+00

    -4.50

    -4.00

    100.00
    -3.50

    a (semilog)
    0.00

    7246

    4.50

    10.00
    20.00
    30.00
    40.00
    50.00
    60.00
    70.00
    80.00
    90.00
    -4.00

    100.00
    -3.50

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