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    V. Data Pengamatan A. Data Absorbansi Baku Sulfametoksazol Konsentrasi (G/ML) Absorbansi

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    Diana Sandra D
    The document presents data from an observation study containing: 1) Standard curves for sulfamethoxazole absorbance and concentration; 2) Absorbance data from samples administered 1000 mg, 1300 mg, and 1500 mg doses of sulfamethoxazole; 3) Calculations of sample concentrations, elimination rate constants, and half-lives for each dose. Regression equations are determined for the standard curves and sample concentration curves. Half-lives are estimated to be around 30-32 minutes for the 1300 mg and 1500 mg doses.

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    V. Data Pengamatan A. Data Absorbansi Baku Sulfametoksazol Konsentrasi (G/ML) Absorbansi

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    Diana Sandra D
    22 views6 pages
    The document presents data from an observation study containing: 1) Standard curves for sulfamethoxazole absorbance and concentration; 2) Absorbance data from samples administered 1000 mg, 1300 mg, and 1500 mg doses of sulfamethoxazole; 3) Calculations of sample concentrations, elimination rate constants, and half-lives for each dose. Regression equations are determined for the standard curves and sample concentration curves. Half-lives are estimated to be around 30-32 minutes for the 1300 mg and 1500 mg doses.

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    The document presents data from an observation study containing: 1) Standard curves for sulfamethoxazole absorbance and concentration; 2) Absorbance data from samples administered 1000 mg, 1300 mg, and 1500 mg doses of sulfamethoxazole; 3) Calculations of sample concentrations, elimination rate constants, and half-lives for each dose. Regression equations are determined for the standard curves and sample concentration curves. Half-lives are estimated to be around 30-32 minutes for the 1300 mg and 1500 mg doses.

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    22 views6 pages

    V. Data Pengamatan A. Data Absorbansi Baku Sulfametoksazol Konsentrasi (G/ML) Absorbansi

    Uploaded by

    Diana Sandra D
    The document presents data from an observation study containing: 1) Standard curves for sulfamethoxazole absorbance and concentration; 2) Absorbance data from samples administered 1000 mg, 1300 mg, and 1500 mg doses of sulfamethoxazole; 3) Calculations of sample concentrations, elimination rate constants, and half-lives for each dose. Regression equations are determined for the standard curves and sample concentration curves. Half-lives are estimated to be around 30-32 minutes for the 1300 mg and 1500 mg doses.

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    of 6

    V.

    DATA PENGAMATAN
    A. Data Absorbansi Baku Sulfametoksazol
    Absorbansi
    0
    0,080
    0,122
    0,141
    0,189
    0,398

    Konsentrasi (g/ml)
    0
    10,84
    21,68
    43,36
    86,72
    119,28
    a

    : 0,0434

    Persamaan Regresi Linier :

    : 2,5299 . 10-3

    y=bx+a

    : 0,9247

    y = 2,5299 . 10-3 x + 0,0434

    Kurva Baku

    Kurva (g/ml) C vs Absorbansi


    1.2
    1
    0.8
    0.6

    y = 1,5034 . 10-3 x + (0,2021)

    0.4

    Linear (y = 1,5034 . 10-3 x +


    (- 0,2021))

    0.2
    0
    0

    100

    200

    300

    400

    500

    600

    700

    800

    -0.2

    B. Data Absorbansi Sampel


    Sampel SMZ
    t (menit ke-)

    Absorbansi

    Dosis 1000 mg
    0,092
    0,110
    0,239
    0,240
    0,310
    0,194
    0,070
    0,062
    0,048

    10
    20
    30
    45
    60
    90
    120
    180
    210

    Dosis 1300 mg
    0,053
    0,056
    0,058
    0,070
    0,073
    0,070
    0,047
    0,046
    0,044

    Dosis 1500 mg
    0,057
    0,193
    0,357
    0,432
    0,141
    0,078
    0,070
    0,020
    0,013

    VI. PERHITUNGAN
    a. Pembuatan Larutan Stok SMZ 1 mg/ml atau 1000 g/ml

    Penimbangan SMZ
    Berat kertas + zat = 0,3525 g
    Berat kertas + sisa = 0,2582 g
    Berat zat

    = 0,0943 g = 94,3 mg

    Konsentrasi larutan stok SMZ sebenarnya

    102,5 mg
    Kadar =

    100 ml

    = 0,943 mg/ml = 943 g/ml

    Deret Baku SMZ


    No
    1.

    Kadar
    0 g/ml ( blangko )
    V1. C1 = V2 . C2

    Koreksi Kadar
    V1. C1 = V2 . C2

    250 l . 0 g/ml = V2 . 1000 g/ml

    250 l . C1 = 0 l . 1025 g/ml

    V2 = 0 l

    C1 = 0 g/ml

    Darah = 250 l 0 l = 250 l


    2.

    100 g/ml
    V1. C1 = V2 . C2
    250 l . 100 g/ml = V2 . 1000 g/ml

    V1. C1 = V2 . C2
    250 l . C1 = 25 l . 1025 g/ml

    V2

    = 25 l

    C1 = 102,5 g/ml

    Darah = 250 l - 25 l = 225 l


    3.

    200 g/ml
    V1. C1 = V2 . C2
    250 l . 200 g/ml = V2 . 1000 g/ml
    V2

    4.

    = 50 l

    V1. C1 = V2 . C2
    250 l . C1 = 50 l . 1025 g/ml
    C1 = 205 g/ml

    Darah = 250 l 50 l = 200 l


    300 g/ml
    V1. C1

    = V2 . C2

    250 l . 300 g/ml = V2 . 1000 g/ml


    V2 = 75 l

    V1. C1 = V2 . C2
    250 l . C1 = 75 l . 1025 g/ml
    C1 = 307,5 g/ml

    Darah = 250 l 75 l = 175 l


    5.

    400 g/ml
    V1. C1

    = V2 . C2

    250 l . 400 g/ml = V2 . 1000 g/ml


    V2 = 100 l

    V1. C1 = V2 . C2
    250 l . C1 = 100 l . 1025 g/ml
    C1 = 410 g/ml

    Darah = 250 l 100 l = 150 l


    6.

    500 g/ml
    V1. C1

    = V2 . C2

    250 l . 500 g/ml = V2 . 1000 g/ml


    V2 = 125 l

    V1. C1 = V2 . C2
    250 l . C1 = 125 l . 1025 g/ml
    C1 = 512,5 g/ml

    Darah = 250 l 125 l = 125 l


    7.

    600 g/ml
    V1. C1 = V2 . C2
    250 l .600 g/ml = V2 . 1000 g/ml
    V2 = 150 l

    V1. C1 = V2 . C2
    250 l . C1 = 150 l . 1025 g/ml
    C1 = 615 g/ml

    Darah = 250 l 150 l = 100 l


    8.

    700 g/ml
    V1. C1 = V2 . C2

    V1. C1 = V2 . C2

    250 l .700 g/ml = V2 . 1000 g/ml

    250 l . C1 = 175 l . 1025 g/ml

    V2 = 175 l

    C1 = 717,5 g/ml

    Darah = 250 l 175 l = 150 l


    Data Konsentrasi Sampel dengan SMZ 1000 mg
    t

    Absorbansi

    (menit ke-)
    10
    20
    30
    45
    60
    90
    120
    180
    210

    0,092
    0,110
    0,239
    0,240
    0,310
    0,194
    0,070
    0,062
    0,048

    = 5,9220

    = - 0,0244

    = - 0,9332

    =-b
    = 0,0244

    t eliminasi = 0,693/ 0,0244


    = 28,40 menit
    = 0,47 jam

    Konsentrasi
    (g/ml)
    19,2200
    26,3350
    77,3257
    77,7209
    105,3903
    59,5382
    10,5239
    7,3617
    1,8278

    Ln Cp
    2.956
    3.271
    4.3480
    4.3531
    4.6577
    4.0866
    2.3536
    1.9963
    0,6031

    Kurva Dosis SMZ 1000 mg


    Data Konsentrasi Sampel dengan SMZ dosis 1300 mg
    t (menit ke-)

    Absorbansi

    Konsentrasi (g/ml)

    Ln Cp

    10
    20
    30
    45
    60
    90
    120
    180
    210

    0,053
    0,056
    0,058
    0,070
    0,073
    0,070
    0,047
    0,046
    0,044

    3,804
    4,990
    5,781
    10,524
    11,710
    10,524
    1,4326
    1,0373
    0,2467

    1,3360
    1,6074
    1,7545
    2,3536
    2,4604
    2,3536
    0,3594
    0,0366
    -1,3996

    A= 3,8851
    B= - 0,0228
    r= -0,8338
    k= -b
    = 0,0228
    T eliminasi = 0,693/k
    = 0,693/ 0,0228
    = 30,3948 menit
    = 0,50658 jam

    Data Konsentrasi Sampel dengan SMZ dosis 1500 mg


    t (menit ke-)
    10
    20
    30
    45
    60
    90
    120
    180
    210

    Absorbansi

    Konsentrasi (g/ml)

    0,057
    0,193
    0,357
    0,432
    0,141
    0,078
    0,070
    0,020
    0,013

    A= 4,8233
    B= - 0,02166
    k = -B
    = 0,02166
    T1/2 eliminasi = 0.693/0.02166
    = 31,994 menit = 0.53 jam

    5,3853
    59,1429
    123,9682
    153,6140
    38,5885
    13,6861
    10,5239
    -9,2398
    -12,000

    Ln Cp
    1,6836
    4,0799
    4,8200
    5,0344
    3,6530
    2,6163
    2,3536
    -

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