Gen HKT 1.

3
>Genome
CAATCATAGAATAGCTCAAATATGTTCAGTAGATCTTGTCTTTAACTTCTTCAATACACC
AAGCTTATTGGGCTAATTTTGTGTTCCAGAACAAAATGTCAGACTTTACCTTCAAGAAAA
AGGGAAAGAAAACACTGTTTGTAGGGAGACAAATATTAGCATATTATTTAGTAACCAAAG
ACGTGATTCTAGTAGTTTCTAATACTACTTCCGTCCTTTAATATATGACATTGATTATTT
CAAAATTGAACTAACCAACGTCATACATAAAAGAACAGAGGGAGTATCAATTAGGATAGC
AAAAATAGGCTTTCAAACCCATATTTCATAAGAGTAACCGCTTGCAACTGTATATGCATA
GTTACAGATATTAAGATGGAATAAATTACTCTGAGTAAAATGGAACAGGTAAACCTCTTC
TCGCATATAGCATTGTATCTCTCTAAAACAGACTTGCAGTACATTATGAGTTTATGACCA
AATCATCTCACCGATTCAATTTTGTAAAATTTAATGCTCTAATTGGATCTAACGCCAATA
AAACTTATGCAAAAAATATTTCATGTATTGACTGTTCAGTTCTAGAATTCCCCTATTTCC
CTCCTATCAAAAACAAGTCAATTTTTTTCAATTCAAAGTCAACAAATGCCGCTTTGACCA
CTGAAATTGTTTACTTGAAAAGAGATTTTAAAATGTAGTTTGATTGAAATTTTTACTCAT
ATCTTTTCATTTCATGCTTCGAAACCATATATTCAAGACAATATATTAAAGTAGAGGCTT
AACAACAATAATGCCCACATCCTGAAATGGCATGAGGTGCAATATTCAAGGTACAATTTT
AGGGTAGTCAAGCAAAAGTACATCACTAGTGATCAGTTGCTGAGAACATATTAGCTTTCT
AACTGAATTCGAACTCTTGAAAGCAGTGCATGTCCAACATGCTGAAACTCACAGATCAAA
GAAAGCATAAGTGTGCGTGCGTTGTGAGTGTCAGCATTGTACTGTGTAATTCTCAAAAAA
AAAAGCATATATTTCATCAGAAGGCAGCCTAATGAAAAGCACATACTGAAATATCATTGC
ATTCGGGGTGCTAATTCTTTGTTCTGCATTTTTCACTAACCTGTATAATCTTTTCAGAAC
AGACTGTTATTTGCCACAATCGCAGTAGAGAATAAATCCTTCAGCACTTCATTTACATTC
ACATAAGCTATATGGAATCCAAAGCACTCTTTGATTCTTCTGTCATTGTTGTCCAGTAAG
TTCACCACTAACTCTTTGATGCTGATTGCTTTTGTTCTACAGCAGAGATTATATGTACGA
ACATCACAAGTATAAGTTGGAGAGGAGAATATTTTTTTCCCTATATAAATGGACAAACAT
CAGTACATTGATGCAAATAAGGAAGCACCCAATACATGCTGTTTAGCTCCACACAAATAG
TTAGAAAATGAATCATTGTCTTGTAGTATCCCACAAAAAACTCCAAACTTTCCGCACATT
TGCAGCTAGCAAGTTCTCTTCTTTTACCAAATCTGCACAGAAGTCTATAAAATACTCCTT
CCAGTTCATCTACCAAAACAATCCACTCTTTGTCCATGTAGCTTACTTTGCCCTGATCTC
CTTTGCTGGATATGGATCTCTAAAGGTCCTCAAGCCACGAGACAAGTCAAATACTCTGAA
AGACTTGGACGTGCTATTTACTTCCGTATCTGCATCAACTGTTTCAAGCATGGCTACTGT
TGAAATGGAGGATTTCTCAAGCGCTCAACTCTGGGTTTTGACTATTTTAATGCTGATTGG
TGGTGAGGTATTCACTTCAATGCTTGGCATTCACTTTATGAGAGCCGAATTTGGTACAAA
AGAGTCAGTCAGCACAAGGGATCACTCACCTTGCATTGATATTGAGTCTATTACTTCCAC
AAAATTTGGTCCCAGCACCCAGGGCACAAAAGTTACAGTTTCATTTTCTGAACTCCGCAT
GGAAAATGGAGGACATGTAGAGCCCAAGACGATTAAATTTTTAGGTTTTGTAGTGATGGG
ATATCTTCTAATAACAAACTTAGGCGGCTCCCTACTTATTTACCTCTACCTTAACCTGGT
ACCAAGTGCACATAAAATTCTAAAGAGAAAAGGCATTGGGATCATCGTATTCTCAGTATT
TACAGCCATCTCCTCAGTTGGAAATTGTGGCTTCACTCCAGTAAATGAGAATATGATTAT
CTTTCAGAAGAACTCCATTCTTCTATTGCTAATTCTTCCTCAGATACTAGCAGGAAATAC
ATTATTTGCACCATGCTTGAGATTAATGGTGTGGTCACTTGAGAAGATTACCGGAAAAAA
GGATTGTCGTTACATTCTTGAATATCCAAAGGCCATTGGATATAAACATCTTATGAGTAC
CAGGGAAAGTGTTTATTTGACTTTAACAGTTGTGAGCTTGATCATTCTGCAAACCGTATT
GTTCCTCTCTTTGGAGTGGAGCTCGGTAGCTTTGGATGGAATGAGCAACTATCAAAAGAT
AGTATCCGCTCTATTTCAGTCGGTCAATGCTAGGCATGCAGGTGAATCTGTTACAGATCT
GTCAAACCTCTCTTCAGCAATCCTAGTCCTATACACCATCATGATGTAAGTCCCTTCTCA
AACCTTCTCCCCAAAATAAGAATGATCATAAGTAAGAAAACTTCCTGTATTTATTGCTCT
AGTCAGTAAACTATATAATTTAACTACCTTATCATGGACATCACATTTGCCTTCCTTCAC
TTATAATAGCCCTATAGTGGTTCTCTCGAATATGTGCAGCAACGTTCAAATATATTGTAC
TTCACTAATAGGTAGGTTGCATATAGTAGAATGGAACATAAGGACATCAGATAATCTTTA
AGAATACACAGGAAAGCTTCCTACCATAGTACTCCTACCTATTAAGAAAGGAATTTTTTA
ATCACACAATCCCACAAGCCAGACTCAGTGCTAGATTTGGTTGAGGACTATAGTAGTTCA
AGAACCACTTACAGGCTCAACAGTATATTACATACAAAGCAGTTAGTCCAGTATATGTTA
TCATTGAGAGGAATTGTTCTTAATATGTAATCTTAACACATACTTTGACTAATTTTTATG
TAGGTATCTCCCTGGTTACACTTCGTTTTTACCCAGACATGATGGTGAGGATTCTAAGAC

CGAGAAGATAAACAAAAGAAAAGGGCTATTGGAGAACTGGATCTTCTCACATATGTCTTA
TTTGGCTATCTTTGTAATGCTAATTTGCATCACAGAACGGGACTCGATGGCTACAGATCC
ACTTAATTTCAATGTTTTCAGCATATTGTTTGAAGTCGTCAGGCAAGCCACTGAAATTCA
CTGCATTCTGCAGAAAGCTCATGATTATTTTTTAACATGAGTATCATCACTGAACATCTG
GCTCTGATTGTTGCAGTGCATATGGAAATGTGGGGTTCTCGGTTGGCTACAGCTGCAAGA
GGCTACTGAACCATGATGCACGCTGCAAGGATGCCTCGTACGGGTTTGCGGGGAAATGGA
GCGACAATGGGAAAGCGATCCTGATCATCGTCATGCTTTTCGGGAGGCTTAAAACGTTTA
ACATGAAGGGTGGAAGAGCCTGGAAGCTTAGATAACATCTAACCAATTTCAGAATAAAAG
TAGCTAAATAATGCTGAACAGCTTCAGGTGTACACAACAATGTGCTAGCGACAGATTCGA
GCTTAGCTTAGAATAACAAGGGTGATTAGGTGAAAGATGCTCGTCAGCATTATGTGGCCT
TTCATTCACCAAGAAGCCATGCATCTTCCACCTGTGCTAAAATGAACTACAAAAAAAAAA
TTGTATTCCAGTTTCTTTTTGTGATAGCCTCCATAGTTCTCTACACCAATTCCAACTTCC
AATTCATCCAGCAAAATATTGGGGGTTTTTTTTTTCGAACAACAGCTTCACGCATTCAGA
GAAGAGGAGAGAATTCGAAACGACTCGCACAAATCCCCAACATGACTGGATGCAACGAAT
CGAACGCCAAATGCACATGGACCTAGCTTTTCAGATCTGAAGCAACGGCTAAGGATGCAG
CAGAGCAATCGAGTAGGGGAATGCATTAGTGAAGGAGGATGGGTTCACGGAGCGTACATA
CATCTCGTCGGGCGGCGAAGGGAAGCTCTCGCATGGCGGCGGCGGCGCGAGCCGGCGACC
TCGATAGCGAGCGGGCGGTCGCCGACGACGGCGATGATGGTTCAGGCAGCCTACTGCTAG
TGCTACGGTTGGGCACGGCCCAATCAATAGGGCCCAATTGGCCCGGTTTACTTTAGCCCT
CATTAGGATAGTGAGTCTTTTACTGTGGGTTTTGGAATTAATCCTTCCTACACGTAAAAC
GAGACATGGATTAATGTGTGATTAATTAAGTACTTCCTCCGTAAAAAAAACTAACCTAAT
TGGTGATTTGACACCACCTAAAACAATGAATCTGGACATGGACATATGGTTGTCCAAGTT
CGTAGTCCTAGGTAATGTTAAACCCTCATTTAGGTTGCTTTTTTTTACGGATAGAGTATG
AGTTAAAAAACTTAAAAATAAATTAATATAATTTTTAAAATATAGTATTTTTAATATAAA
CTTTCTAGAAAACACATTGTTTAGCCGTTTAAAAACATGTGCCATCGCCCACCACCTTAT
TTGATTGCAATACTGTAGTTTTCCACTCGTTGATGGAGGTTCTATCTTTGTAGATGCCAT
ACGGGCAGTGGGGAACTTGACGGGTGCCCCCGCTTCCCGTACCCGCATGGGGAGGATGAT
GGGGAAATTTGTCCTTTATTTTTAACATAAACTGTTGTTTTGCTCTCTCTTATTAGGGTT
TGTATTTTTGCCTTTATTTTTTAAAAGGAATCAGTCGTTTGCCTCCACCTTTGATGTCTG
GTTATATGGGCCCCATGGATTTTAGTTAAATCTATATATATATATATATATATATAGCTA
ATTAAAAAATACGTAAGGTT

>CDS(3 on:1446>2628;3426>3343;3436>3636)
ATGAATCATTGTCTTGTAGTATCCCACAAAAAACTCCAAACTTTCCGCACATTTGCAGCT
AGCAAGTTCTCTTCTTTTACCAAATCTGCACAGAAGTCTATAAAATACTCCTTCCAGTTC
ATCTACCAAAACAATCCACTCTTTGTCCATGTAGCTTACTTTGCCCTGATCTCCTTTGCT
GGATATGGATCTCTAAAGGTCCTCAAGCCACGAGACAAGTCAAATACTCTGAAAGACTTG
GACGTGCTATTTACTTCCGTATCTGCATCAACTGTTTCAAGCATGGCTACTGTTGAAATG
GAGGATTTCTCAAGCGCTCAACTCTGGGTTTTGACTATTTTAATGCTGATTGGTGGTGAG
GTATTCACTTCAATGCTTGGCATTCACTTTATGAGAGCCGAATTTGGTACAAAAGAGTCA
GTCAGCACAAGGGATCACTCACCTTGCATTGATATTGAGTCTATTACTTCCACAAAATTT
GGTCCCAGCACCCAGGGCACAAAAGTTACAGTTTCATTTTCTGAACTCCGCATGGAAAAT
GGAGGACATGTAGAGCCCAAGACGATTAAATTTTTAGGTTTTGTAGTGATGGGATATCTT
CTAATAACAAACTTAGGCGGCTCCCTACTTATTTACCTCTACCTTAACCTGGTACCAAGT
GCACATAAAATTCTAAAGAGAAAAGGCATTGGGATCATCGTATTCTCAGTATTTACAGCC
ATCTCCTCAGTTGGAAATTGTGGCTTCACTCCAGTAAATGAGAATATGATTATCTTTCAG
AAGAACTCCATTCTTCTATTGCTAATTCTTCCTCAGATACTAGCAGGAAATACATTATTT
GCACCATGCTTGAGATTAATGGTGTGGTCACTTGAGAAGATTACCGGAAAAAAGGATTGT
CGTTACATTCTTGAATATCCAAAGGCCATTGGATATAAACATCTTATGAGTACCAGGGAA
AGTGTTTATTTGACTTTAACAGTTGTGAGCTTGATCATTCTGCAAACCGTATTGTTCCTC
TCTTTGGAGTGGAGCTCGGTAGCTTTGGATGGAATGAGCAACTATCAAAAGATAGTATCC
GCTCTATTTCAGTCGGTCAATGCTAGGCATGCAGGTGAATCTGTTACAGATCTGTCAAAC
CTCTCTTCAGCAATCCTAGTCCTATACACCATCATGATGTATCTCCCTGGTTACACTTCG
TTTTTACCCAGACATGATGGTGAGGATTCTAAGACCGAGAAGATAAACAAAAGAAAAGGG
CTATTGGAGAACTGGATCTTCTCACATATGTCTTATTTGGCTATCTTTGTAATGCTAATT
TGCATCACAGAACGGGACTCGATGGCTACAGATCCACTTAATTTCAATGTTTTCAGCATA

TTGTTTGAAGTCGTCAGTGCATATGGAAATGTGGGGTTCTCGGTTGGCTACAGCTGCAAG
AGGCTACTGAACCATGATGCACGCTGCAAGGATGCCTCGTACGGGTTTGCGGGGAAATGG
AGCGACAATGGGAAAGCGATCCTGATCATCGTCATGCTTTTCGGGAGGCTTAAAACGTTT
AACATGAAGGGTGGAAGAGCCTGGAAGCTTAGATAA

Mi 1
Template
Length Start Stop Tm
strand

Sequence (5'->3')

Forward
GTCCAGTAAGTTCACCACTAAC Plus
primer
Reverse
AGAGGTAAATAAGTAGGGAGCC Minus
primer
Product
837
length
Products on intended target

GC%

Self
Self
complementarity com

22

1251 1272 56.40 45.45

4.00

3.00

22

2087 2066 56.41 45.45

2.00

2.00

>NC_008395.2 Oryza sativa Japonica Group DNA, chromosome 2, complete sequence, cultivar:
Nipponbare

product length = 837

Features associated with this product:
hypothetical protein
Forward primer
Template

1
4103242

GTCCAGTAAGTTCACCACTAAC
......................

22
4103263

Reverse primer
Template

1
4104078

AGAGGTAAATAAGTAGGGAGCC
......................

22
4104057

Mi 2

A CAGGTCATTC
Template
Length Start Stop Tm
strand

Sequence (5'->3')
Forward
AAAAGAGTCAGTCAGCACAAGG
primer
Reverse
GCATTGACCGACTGAAATAGAG
primer
Product
693
length
Products on intended target

GC%

Self
Self 3'
complementarity complementarity

Plus

22

1858 1879 58.79 45.45 3.00

0.00

Minus

22

2550 2529 57.21 45.45 3.00

1.00

>NC_008395.2 Oryza sativa Japonica Group DNA, chromosome 2, complete sequence, cultivar:
Nipponbare

product length = 693

Features associated with this product:
hypothetical protein
Forward primer

AAAAGAGTCAGTCAGCACAAGG

22

Template

4103849

......................

4103870

Reverse primer
Template

1
4104541

GCATTGACCGACTGAAATAGAG
..................

22

Mi 3(trng 1 on mi R2)
Template
Length Start Stop Tm
strand

Sequence (5'->3')
Forward
GCGGCTCCCTACTTATTTAC
primer
Reverse
CTGCACATATTCGAGAGAACC
primer
Product
736
length
Products on intended target

GC%

Self
Self 3' complemen
complementarity

Plus

20

2064 2083 55.72 50.00 3.00

0.00

Minus

21

2799 2779 56.52 47.62 4.00

0.00

>NC_008395.2 Oryza sativa Japonica Group DNA, chromosome 2, complete sequence, cultivar:
Nipponbare

product length = 736

Features associated with this product:
hypothetical protein
Forward primer
Template

1
4104055

GCGGCTCCCTACTTATTTAC
....................

Reverse primer
Template

1
4104790

CTGCACATATTCGAGAGAACC
.....................

20
4104074
21
4104770

Mi 4
Template
Length Start Stop Tm
strand

Sequence (5'->3')
Forward
AACCACTTACAGGCTCAACAG
primer
Reverse
CTGACGAGCATCTTTCACCTA
primer
Product
765
length
Products on intended target

GC%

Self
Self 3' complemen
complementarity

Plus

21

3003 3023 58.15 47.62 3.00

1.00

Minus

21

3767 3747 57.49 47.62 3.00

2.00

>NC_008395.2 Oryza sativa Japonica Group DNA, chromosome 2, complete sequence, cultivar:
Nipponbare

product length = 765

Features associated with this product:
hypothetical protein
Forward primer

AACCACTTACAGGCTCAACAG

21

Template

4104994

.....................

4105014

Reverse primer
Template

1
4105758

CTGACGAGCATCTTTCACCTA
.....................

21
4105738