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MTT Assay Mosmann
MTT Assay Mosmann
MTT Assay Mosmann
2004] modiefied
-
5/10/2005
add MTT solution [5 mg/mL] in an amount equal to 10% of the cell culture
volume
incubate at appropriate temperature for the used cell-type for 1-4 hours
MTT-solution:
-
Acetic Isopropanol:
0.1 N HCl in isopropanol
References
La Fontaine S, Firth SD, Lockhart PJ, Brooks H, Parton RG, Camakaris J, Mercer JF
(1998), Functional analysis and intracellular localization of the human menkes protein
(MNK) stably expressed from a cDNA construct in Chinese hamster ovary cells
(CHO-K1), Hum.Mol.Genet. 7: 1293-1300
Mosmann T (1983), Rapid colorimetric assay for cellular growth and survival:
application to proliferation and cytotoxicity assays, J.Immunol.Methods 65: 55-63
Southon A, Burke R, Norgate M, Batterham P, Camakaris J (2004), Copper
homoeostasis in Drosophila melanogaster S2 cells, Biochem.J. 383: 303-309