GROUP 5

2015
BMLS 2B BIOCHEMISTRY LAB 3-5 TTHS R602

October 8,

EXPERIMENT NO. 15: PROPERTIES OF LIPIDS

I. Objectives:
- To know what are lipids and their functions
- To know their occurrence and structure
- To learn how lipids and fatty acids are classified
- To learn their physical and chemical properties

II. Procedure:
A. SOLUBILITY
2 drops coconut oil

1 ml
H20

1 ml
dilute
HCL

1 ml
dilute
NaOH

1 ml
cold
alcoho

1 ml
hot
alcoho

1 ml
ether

B. SPOT TEST
1 drop
coconut
oil

Drop on a piece
of paper

C. REACTION WITH LITMUS PAPER

Litmus paper (previously moistened

Rancid
coconut
oil

Fresh
coconut
oil
D. EMULSIFICATION
1

Few drops of
coconut oil in
1ml H20

1ml H2O
+ 0.5%
Sodium
Carbonate
soln +
coconut

Shake

1 ml
chlorofor
m

1 ml
CCl4

E. ACROLEIN TEST
1 ml
coconut
oil

+ pinch
Potassium
Bisulfate

Heat
gradually

F. TEST FOR UNSATURATION

1.

3ml
Chlorofor
m

Dissolve 5
drops
linseed oil

+
Hubls
soln

Shake until
permanent brownish
orange tinge

Record # of drops
needed to get the
color

REPEAT STEPS BUT REPLACE LINSEED OIL WITH COTTON SEED OIL

G. FAT CRYSTALS
Heat a
small
piece of

Get 1ml of
extract

Dissolv
e in 2ml
ether

Stopper
loosely
with filter
paper

REPEAT STEPS BUT REPLACE PORK FAT WITH BEEF FAT

III. DATA

Allow to
evaporate until
crystals begin to
separate

Examine

A.
In what solvents is the oil soluble? __________________________________
What are these solvents called? _____________________________________
B.
Does it appear? __________
C.
Results: ________________________________________________________________________________
How do you account for the difference in reaction between the fresh and rancid oil?
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D.
1. Result: _________________________________________________________________________________
2. Result: _________________________________________________________________________________
3. What is the effect of sodium carbonate?
_________________________________________________________________________________
E.
To what is it due?
_________________________________________________________________________________
Equation:

F.
3. Number of drops used: _________
4. Number of drops used: _________
What conclusion can you make based on the number of drops of Hubls solution used for each
oil?
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______________________________
What is meant by iodine number? Give its significance.
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______________________
SOURCES:

DRAWINGS: