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    Dipsticks & Biosensor

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    Jsquare
    Glucose dipsticks and biosensors operate on the principles of enzymatic reactions. Glucose dipsticks use immobilized enzymes that react with glucose molecules to produce a colored product, allowing for visual measurement. Glucose biosensors have five main parts: a selectively permeable membrane, biological recognition layer containing enzymes like glucose oxidase, a transducer to convert the enzymatic reaction into an electrical signal, an amplifier, and a signal processing system to provide a digital readout of glucose levels. Biosensors offer advantages like high sensitivity, rapid response, and ability to detect specific molecules in mixtures.

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    Dipsticks & Biosensor

    Uploaded by

    Jsquare
    91 views15 pages
    Glucose dipsticks and biosensors operate on the principles of enzymatic reactions. Glucose dipsticks use immobilized enzymes that react with glucose molecules to produce a colored product, allowing for visual measurement. Glucose biosensors have five main parts: a selectively permeable membrane, biological recognition layer containing enzymes like glucose oxidase, a transducer to convert the enzymatic reaction into an electrical signal, an amplifier, and a signal processing system to provide a digital readout of glucose levels. Biosensors offer advantages like high sensitivity, rapid response, and ability to detect specific molecules in mixtures.

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    Glucose dipsticks and biosensors operate on the principles of enzymatic reactions. Glucose dipsticks use immobilized enzymes that react with glucose molecules to produce a colored product, allowing for visual measurement. Glucose biosensors have five main parts: a selectively permeable membrane, biological recognition layer containing enzymes like glucose oxidase, a transducer to convert the enzymatic reaction into an electrical signal, an amplifier, and a signal processing system to provide a digital readout of glucose levels. Biosensors offer advantages like high sensitivity, rapid response, and ability to detect specific molecules in mixtures.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as ppt, pdf, or txt
    91 views15 pages

    Dipsticks & Biosensor

    Uploaded by

    Jsquare
    Glucose dipsticks and biosensors operate on the principles of enzymatic reactions. Glucose dipsticks use immobilized enzymes that react with glucose molecules to produce a colored product, allowing for visual measurement. Glucose biosensors have five main parts: a selectively permeable membrane, biological recognition layer containing enzymes like glucose oxidase, a transducer to convert the enzymatic reaction into an electrical signal, an amplifier, and a signal processing system to provide a digital readout of glucose levels. Biosensors offer advantages like high sensitivity, rapid response, and ability to detect specific molecules in mixtures.

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    © All Rights Reserved
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    of 15

    Glucose Dipstick &

    Biosensor

    S(f) Explain the principles of operation

    Quantitative measurement of
    glucose
    A diabetic patient:

    blood glucose level > 4.7 mM


    Urine glucose level > 11 mM

    Need a rapid test to monitor

    when & how much insulin


    needs to be injected.

    Glucose Dipstick

    Glucose Dipstick - principles

    Glucose Biosensor

    Biosensor principles
    Five major parts:
    1.
    2.
    3.
    4.
    5.

    Selectively permeable membrane


    Biological recognition layer
    Transducer
    Amplifier
    Signal processing system

    Glucose Biosensor
    principles
    At biological recognition

    layer:
    Glucose + O2 + H2O
    glucose oxidase

    gluconic acid + H2O2


    At negative electrode:

    O2 +4e- +4H+
    electric signal
    digital readout

    2H2O

    Biosensor - Advantages
    Can detect specific molecule in a mixture
    Very sensitive,

    can detect small sample and [low]

    Rapid in response
    Safe to use
    Accurate
    Small
    Can be mass produced.

    Biosensor - Disadvantages
    need careful handling
    not very stable
    not sterilisable

    Figure- Cyanide biosensor

    Biosensor Applications
    Medical

    Cholesterol & alcohol measurement

    Food industry

    Microbial detection in meat,


    dairy products & water

    Agriculture

    Pesticides, crop disease, ripeness, nutrients

    Environment

    Air and water pollutants

    Food Safety Biosensor

    Biosensors in Agriculture

    Water Toxicity Biosensor

    Principles of Glucose Dipstick


    & Biosensor (recap)
    Glucose dipstick
    Immobilised enzymes
    react with glucose molecules,
    product is coloured.
    Glucose biosensor
    1.
    2.
    3.
    4.
    5.

    Selectively permeable membrane


    Biological recognition layer
    Transducer
    Amplifier
    Signal processing system

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